Alignment viewer

BLASTN 2.6.0+ Reference: Stephen F. Altschul, Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Database: Raffaelea_20quercivora_20[genbank_20GCA_5F001662465.1_202016-06-08] 26 sequences; 25,862,907 total letters Query= NC_007921.1 Length=147544 Score E Sequences producing significant alignments: (Bits) Value seq2 295 6e-75 seq1 134 1e-26 seq3 116 3e-21 seq10 111 1e-19 >seq2 Length=4288194 Score = 295 bits (326), Expect = 6e-75 Identities = 969/1474 (66%), Gaps = 43/1474 (3%) Strand=Plus/Minus Query 144914 ATTTCCCAAGCGGTCTTGTACAAACGTTTAATGTGGTCCGGAATGCCGTCAATCGCCTGA 144973 || ||||| | |||||||||| || |||| | |||| ||| | ||| ||| Sbjct 3317489 ATCTCCCACACCGTCTTGTACAGGGCCTTGATGTCGGCCGGGATGTTGGGAATGTTCTGG 3317430 Query 144974 ATCGACCCCTTGTGCGCAAATATGGCGTTCCGCATTTCGGACGTGTACAGTTTTAACTTG 145033 ||||| || || | ||||| ||| |||| ||| | | || |||| | | | Sbjct 3317429 ATCGAACCGTTCTCCGCAATGATGCGGTTCTTCATGTTGTCCGACCACAGACCCATGTCG 3317370 Query 145034 ATCAAGTCGTCAATTAAGTACTGATTGACGACCTGAAACTCGCCGGCCAACACACGACGC 145093 | || ||| | | | || | ||||| ||||| ||||||||||||| |||||| ||| Sbjct 3317369 ACCAGGTCCTTGAGCAGCCACGGGTTGACCACCTGGAACTCGCCGGCCAGCACACGCCGC 3317310 Query 145094 AAATACACGTTAGACGTGAAGGGTTCAAAAGACTCGTTATTGCCCAGGATTTGAGCGGTC 145153 | || | ||| |||||| | || || || ||||||| |||||||| || || | ||| Sbjct 3317309 GAGTAGATGTTGGACGTGTATGGCTCGAAGCACTCGTTGTTGCCCAGAATCTGCGACGTC 3317250 Query 145154 GTTGCGGTGGGCATGTACGCCACCA--ATAATGAATTGCGCAGGCCGTGCTTCTTGATGT 145211 | || || ||||| |||||||| | | || ||||||| ||||||| |||||| Sbjct 3317249 GACGCCGTCGGCATCGGCGCCACCAGCAGACTG--TTGCGCACACCGTGCTCCTTGAT-- 3317194 Query 145212 CTTC--CTTTAGCGTACCCCAATCCCATAGCGAAGTCGGCCTCACGTCCCACATGTCGTA 145269 |||| | |||| |||| ||||| || | ||| ||||||||||||||||| Sbjct 3317193 CTTCAGCCGCAGCGAGTCCCAGTCCCACAGGTCCGAGGGCGTCACGTCCCACATGTCGGG 3317134 Query 145270 CTGCAAAATGCCCTCGCTGGCGGGGCTGCCTTGAAAGGAACTGTAACTGCCCCAAGTTTT 145329 ||||| |||||||| | || ||| | | | | ||| | ||| | || Sbjct 3317133 CTGCAGAATGCCCTGCGACACAGGAGAGCCCTCATATGTTTGGTAGGTACCCTCGATCTT 3317074 Query 145330 GGCGAGCTCGCAACTCGCCTCCAGAGCGCCGAAATAAATTGTCTCGGCAATTTGCCTGTT 145389 ||| ||| |||| | || ||| || || || || |||||| || ||| |||| Sbjct 3317073 GGCCAGCGCGCACGACTCCGTCAGCGCCGCGTGGTAGATGGTCTCGAAGATCTGCTTGTT 3317014 Query 145390 GAGCAGCATCGCTTCATCGCTCTCGTACGGCATGCGCAGCAGTACAAAAGCATCGGCGAG 145449 ||||| || || ||| | |||||||||||| | || || || || || Sbjct 3317013 CAGCAGGCGTGCCTCGGGCGACTCAAAGGGCATGCGCAGCGCCAGGAAGGCGTCTGCCAG 3316954 Query 145450 CCCCTGTATTCCCACGCCGATGGGCCGATGTTTCATGTTGCTCATCCTCGCCGACTCG-- 145507 ||||| | |||| || |||||||| || ||||||||| | ||| | |||| Sbjct 3316953 ACCCTGCACACCCAGACCAATGGGCCGGTGGCGCATGTTGCTGTT--TCG-TGCCTCGGG 3316897 Query 145508 -ATAGGATA-TCGATTACGGTCGATAATTTTATTCAAGTTTCGCACTACTACCTTTGTAA 145565 | || || | | | || ||| || || || | ||| || || || | || || | Sbjct 3316896 CACGGGGTAGTAGTTCAC-GTCAATGATGCGGTTGAGGTTGCGGACAACCAACTGCGTGA 3316838 Query 145566 TGTGCCGCAACTTTTCAAAGTCAAACCGAGCCTCGTCAACGACCACACAGCTATTGACG- 145624 || ||| | | |||||| || | | || | | | || | | | || | Sbjct 3316837 CGTCGTGCAGCCGCTGGAAGTCATACGACTCGTTGTTATAGTCGAC-GAACGACGGAAGA 3316779 Query 145625 GCGATCGACGCCAAATTACAAACTGCCGTCTCCT-CGGCGTCGCTGTATTGTACGATCTC 145683 || | ||| ||||| || || || ||| ||| | ||||| || ||| | || ||||| Sbjct 3316778 GCCAGCGAGGCCAAGTTGCACACGGCCACCTCGTCCGGCGCCG-AGTACTCCACAATCTC 3316720 Query 145684 GGCGCACAAATT---GCTACATTTGATGGTTCCCAGATTTTGCTGATTACTTTTCCTGTT 145740 | ||||| || ||| | |||||| ||||| || | ||| || || || | ||| Sbjct 3316719 CGTGCACAGGTTCGAGCTGC---GGATGGTGCCCAGGTTCTTCTGGTTGCTCTTGCGGTT 3316663 Query 145741 GCACGCGTCCTTGTAGAGCATGTACGGTCCGCCAGTCTCCACCTGCGTCTCCACGATAAA 145800 ||| |||||||||||||||| |||| ||| |||||| ||||| ||||| || | Sbjct 3316662 GCAGTGGTCCTTGTAGAGCATGAACGGGTTGCCCGTCTCCGTCTGCGCCTCCA-GA-ATG 3316605 Query 145801 ACGGTGCA-AG-TCCTGCGCACGCACCTGCTGCTGGTAGCGGCCCTCTTCTTCGTATTTC 145858 || || || | ||| || |||| || | |||||||| ||||| ||| Sbjct 3316604 GCGTACCACAGCTTCTGGGCCTTGACCTTCTTACGACCACGGCCCTCGCTCTCGTACTTC 3316545 Query 145859 CTGTAGAGCTCCTCAAACGCGGAACCGTAGCAATCGGACAGGCCGGGACACTTGTTGGGA 145918 ||| ||| |||||||| | ||||||||||| | |||||||| |||| ||||| Sbjct 3316544 TCGTACAGCGCCTCAAACTCCTCGCCGTAGCAATCCGCCAGGCCGGCGCACTCATTGGGG 3316485 Query 145919 CACATCAGCGACCATACGCCGTTGGCGTTGACGCGCTCCATAAACAAATCTGGCACCCAC 145978 |||||||||| ||| ||||||||| | || |||| ||| |||| ||| || | ||| Sbjct 3316484 CACATCAGCGTCCACTCGCCGTTGGTCTCCACACGCTTCATGAACAGATCGGGGATCCAG 3316425 Query 145979 AGCGCGTACATCAAGTCGCGCGCTTTGCAATCCTCAGCACCCATGTTTCGCTTCAAATTA 146038 || ||| | | || || || | ||||| |||| ||| | || | Sbjct 3316424 AGAGCGAGGAAAAGATCACGGGCACGGACCTCCTCCTTACCCTGGTTCTTCCGCAGGTCC 3316365 Query 146039 AGCACACT-ATAGATGTCCGCGTGCCACGGTTCAATATAGACGGCGAGGGCTCCTTTTCG 146097 || | ||| | ||| || ||||||||||| || | |||| | || ||| || || Sbjct 3316364 AGGA-ACTCAAAGACATCGGCGTGCCACGGCTCCAAGTAGATGCAGAAGGCACCGGGGCG 3316306 Query 146098 ---TTTGCCACCCTGGTCCACGTGACGTACCATGTTGTTAAAGACGCGCACCATGGGCTC 146154 ||||||||||||||||||| || || ||||||||||||| || | ||| || Sbjct 3316305 CTTGTTGCCACCCTGGTCCACGTAGCGGGCCGTGTTGTTAAAGACACGAAGCATAGGGAT 3316246 Query 146155 GACGCCGCTGGCTACGCCGTTCGTAGAGTTTATTCTGGTGCCGCGGGAGCGCACGCAATG 146214 || |||| ||| | |||||| || | || || |||| || ||| | || || Sbjct 3316245 GATGCCGTTGGATGTGCCGTTGGTGCCGGCAATGTAGGAGCCGGTGGCGCGAATGCGGTG 3316186 Query 146215 AACATTCACGCCGAGACCGCC-GCCGTACTTGGATATAGCGGCGCA-GTCGCCGAGCGTC 146272 ||||||| |||| || || ||||| |||||| || ||| || ||| |||||| Sbjct 3316185 GACATTCAGACCGATGCCACCGGCCGT-CTTGGAGATCATGGCACACGTC-TTGAGCGTG 3316128 Query 146273 TCGTAGATGCCGTCAATGCTATCG--CTTCGAATGCCGAGCAGAAAGCACGAGCACATCT 146330 ||||||||||| |||||||| ||| |||| ||| | | || ||||| || ||| || Sbjct 3316127 TCGTAGATGCCCTCAATGCTGTCGTCCTTC--ATGTCCACGAGGAAGCAGGAAGACAGCT 3316070 Query 146331 GCGCCATCGGCGTCCCCGCCGAGAACAACGTCGG 146364 | ||| ||||| || || ||| | |||||| Sbjct 3316069 GAGCCTGGGGCGTGCCGGCGTTGAAGAGCGTCGG 3316036 Score = 129 bits (142), Expect = 6e-25 Identities = 166/229 (72%), Gaps = 0/229 (0%) Strand=Plus/Minus Query 131008 CCACCGCGCAAACGCAGCACTAAATGTATGGTCGACTCCTTTTGAATGTTGTAATCGCTC 131067 ||||| || | ||| || || | || | ||| || ||||| || |||||||| ||| Sbjct 328446 CCACCACGGAGACGAAGGACCAGGTGGAGGGTGGATTCCTTCTGGATGTTGTAGTCGGAG 328387 Query 131068 ATGGTGCGCGAGTCCTCTAGCTGCTTGCCTGCGTAGATTAAACGCTGCTGGTCCGGAGGG 131127 | |||||| |||||| ||||||||||| ||| |||| | |||||||||||| || || Sbjct 328386 AGGGTGCGGCCGTCCTCCAGCTGCTTGCCAGCGAAGATAAGACGCTGCTGGTCGGGGGGA 328327 Query 131128 ATTCCTTCTTTGTCGGTGATTTTCTGCTTGAGCTGCTCCACAGTATCGGTGGACTCGATG 131187 || || || ||||| ||| || ||||| | || | ||||| | ||||||| Sbjct 328326 ATGCCCTCCTTGTCCTGGATCTTGGACTTGACGTTGTCAATGGTATCCGAAGACTCGACC 328267 Query 131188 TCGACGGTAACGGTTTTACCGGTTAGAGTTTTTACGAAAATCTGCATGA 131236 || | || | ||| || ||||| || || || |||||||||||||||| Sbjct 328266 TCAAGCGTGATGGTCTTGCCGGTGAGCGTCTTGACGAAAATCTGCATGA 328218 Score = 105 bits (116), Expect = 6e-18 Identities = 160/228 (70%), Gaps = 0/228 (0%) Strand=Plus/Minus Query 131007 GCCACCGCGCAAACGCAGCACTAAATGTATGGTCGACTCCTTTTGAATGTTGTAATCGCT 131066 |||||| |||| || || || | || | || |||||||| || |||||||| || Sbjct 328675 GCCACCACGCAGGCGGAGAACCAGGTGGAGCGTAGACTCCTTCTGGATGTTGTAGTCCGA 328616 Query 131067 CATGGTGCGCGAGTCCTCTAGCTGCTTGCCTGCGTAGATTAAACGCTGCTGGTCCGGAGG 131126 | |||||| ||||| ||||||||||| || |||| | ||||||||||| || || Sbjct 328615 GAGGGTGCGGCCATCCTCCAGCTGCTTGCCAGCAAAGATCAGGCGCTGCTGGTCTGGCGG 328556 Query 131127 GATTCCTTCTTTGTCGGTGATTTTCTGCTTGAGCTGCTCCACAGTATCGGTGGACTCGAT 131186 || || || ||||| || || ||||| | || | || |||| ||||||| Sbjct 328555 AATGCCCTCCTTGTCCTGAATCTTGCTCTTGACATTGTCGATCGTGTCGGACGACTCGAC 328496 Query 131187 GTCGACGGTAACGGTTTTACCGGTTAGAGTTTTTACGAAAATCTGCAT 131234 |||| || | ||| || ||||| || || || ||||| |||||||| Sbjct 328495 CTCGAGAGTGATGGTCTTGCCGGTAAGCGTCTTGACGAAGATCTGCAT 328448 Score = 100 bits (110), Expect = 3e-16 Identities = 159/228 (70%), Gaps = 0/228 (0%) Strand=Plus/Minus Query 131008 CCACCGCGCAAACGCAGCACTAAATGTATGGTCGACTCCTTTTGAATGTTGTAATCGCTC 131067 ||||| |||| ||||||||| || || | ||||||||||| ||||||||||| ||| | Sbjct 329130 CCACCACGCAGACGCAGCACCAAGTGCAGCGTCGACTCCTTCTGAATGTTGTAGTCGGAC 329071 Query 131068 ATGGTGCGCGAGTCCTCTAGCTGCTTGCCTGCGTAGATTAAACGCTGCTGGTCCGGAGGG 131127 | || || ||| || | ||||||||| || | || | ||||||||||| || ||| Sbjct 329070 AGCGTCCGGCCGTCTTCCAACTGCTTGCCCGCAAAAATCAGGCGCTGCTGGTCTGGCGGG 329011 Query 131128 ATTCCTTCTTTGTCGGTGATTTTCTGCTTGAGCTGCTCCACAGTATCGGTGGACTCGATG 131187 || || || ||||| ||| || ||||| | || | || || | ||||| | Sbjct 329010 ATGCCCTCCTTGTCTTGGATCTTGCTCTTGACGTTGTCAATCGTGTCCGACGACTCAACC 328951 Query 131188 TCGACGGTAACGGTTTTACCGGTTAGAGTTTTTACGAAAATCTGCATG 131235 || | |||| || || || || | || || || || || |||||| Sbjct 328950 TCCAACGTAATCGTCTTGCCCGTCAAGGTCTTGACAAAGATTTGCATG 328903 Score = 100 bits (110), Expect = 3e-16 Identities = 159/228 (70%), Gaps = 0/228 (0%) Strand=Plus/Minus Query 131007 GCCACCGCGCAAACGCAGCACTAAATGTATGGTCGACTCCTTTTGAATGTTGTAATCGCT 131066 ||| || || | ||||||||| | || | ||||||||||| || |||||||| ||| Sbjct 329359 GCCGCCACGAAGACGCAGCACCAGGTGCAGTGTCGACTCCTTCTGGATGTTGTAGTCGGA 329300 Query 131067 CATGGTGCGCGAGTCCTCTAGCTGCTTGCCTGCGTAGATTAAACGCTGCTGGTCCGGAGG 131126 || || || ||| || | ||||||||| || | || | ||||||||||| || || Sbjct 329299 CAGCGTCCGGCCGTCTTCCAACTGCTTGCCCGCAAAAATCAGGCGCTGCTGGTCTGGCGG 329240 Query 131127 GATTCCTTCTTTGTCGGTGATTTTCTGCTTGAGCTGCTCCACAGTATCGGTGGACTCGAT 131186 || || || ||||| ||| ||| ||||| | || | || || | ||| || | Sbjct 329239 AATGCCCTCCTTGTCCTGGATCTTCGACTTGACGTTGTCAATGGTGTCCGAGGATTCCAC 329180 Query 131187 GTCGACGGTAACGGTTTTACCGGTTAGAGTTTTTACGAAAATCTGCAT 131234 || | |||| || || || || || || || |||||||||||||| Sbjct 329179 TTCCAGCGTAATCGTCTTGCCCGTCAGGGTCTTGACGAAAATCTGCAT 329132 Score = 84.2 bits (92), Expect = 2e-11 Identities = 156/229 (68%), Gaps = 0/229 (0%) Strand=Plus/Minus Query 131007 GCCACCGCGCAAACGCAGCACTAAATGTATGGTCGACTCCTTTTGAATGTTGTAATCGCT 131066 |||||| |||| || || || | || | || |||||||| || |||||||| || Sbjct 328903 GCCACCACGCAGGCGGAGAACCAGGTGGAGCGTAGACTCCTTCTGGATGTTGTAGTCCGA 328844 Query 131067 CATGGTGCGCGAGTCCTCTAGCTGCTTGCCTGCGTAGATTAAACGCTGCTGGTCCGGAGG 131126 | |||||| ||||| ||||||||||| || |||| | ||||||||||| || || Sbjct 328843 GAGGGTGCGGCCATCCTCCAGCTGCTTGCCAGCAAAGATCAGGCGCTGCTGGTCTGGCGG 328784 Query 131127 GATTCCTTCTTTGTCGGTGATTTTCTGCTTGAGCTGCTCCACAGTATCGGTGGACTCGAT 131186 ||| || || ||||| ||| || ||||| | || | || || | ||||| | Sbjct 328783 GATGCCCTCCTTGTCTTGGATCTTGCTCTTGACGTTGTCAATCGTGTCCGACGACTCAAC 328724 Query 131187 GTCGACGGTAACGGTTTTACCGGTTAGAGTTTTTACGAAAATCTGCATG 131235 || | |||| || || || || | || || || || || |||||| Sbjct 328723 CTCCAACGTAATCGTCTTGCCCGTCAAGGTCTTGACAAAGATTTGCATG 328675 >seq1 Length=5014741 Score = 134 bits (148), Expect = 1e-26 Identities = 256/366 (70%), Gaps = 11/366 (3%) Strand=Plus/Plus Query 46234 GGCATCATGCCCGGCCTGACGTTTAGCAACGAGCTGATATCGCGCGACGAGGGCCTGCAT 46293 ||| | ||||| || ||||| || ||| |||||||| || ||||||||||||||||| Sbjct 1638501 GGCCTGATGCCTGGTCTGACTTTCTCCAATGAGCTGATTTCCCGCGACGAGGGCCTGCAC 1638560 Query 46294 ACGGACTTTGCGTGTCTCTACTATAACACTCGCGTGCACGACAAGCTTGCGCCCG-ACAC 46352 || |||||||| || || || | | | | | || || || || | || | | | Sbjct 1638561 ACCGACTTTGCCTGCCTG--CTGTT-CTCCCACCTGAACAACC-GCCCGAGCAAGCAGAT 1638616 Query 46353 CGTGCTGAGCATGTTCAAGGAGGCGGTGGCCATTGAGAAGGAGTTTTTTACGGCCGCACT 46412 | | | | ||| || || || || |||||| |||||||||| || | || || Sbjct 1638617 CATCCAGGACATTATCGTCGATGCCGTCAAGATTGAGCAGGAGTTTTTGACCGAGGCCCT 1638676 Query 46413 GCCCGCTGCCCTGCTGGGCATGAACGC-ACAGTCAATGTGCGACTACATCGAGTTTGTAG 46471 |||| ||| || |||||||||||||| || ||| || | ||||| ||||| || | Sbjct 1638677 GCCCTGTGCTCTTCTGGGCATGAACGCCACCCTCATGAAGC-AGTACATTGAGTTCGTTG 1638735 Query 46472 CGGACCGACTGCTCGTGCAGCT-TCACCAGCCG-AAGCATTTTCACGCGCGCAATCCATT 46529 | ||||| ||||| || ||| || || | ||| | | | | |||||| || Sbjct 1638736 CCGACCGTCTGCTGGTC--GCTCTCGGCAACGACAAGGTCTACCGCTCCACCAATCCCTT 1638793 Query 46530 TGAGTTTATGAACAACATCTCCCTGGAGGGCAAAACTAATTTTTTCGAAAAGCGCGTCGG 46589 ||| |||||| | ||||||||||||| ||||| || || || ||||| ||||||||||| Sbjct 1638794 TGACTTTATGGAGAACATCTCCCTGGGCGGCAAGACCAACTTCTTCGAGAAGCGCGTCGG 1638853 Query 46590 CGAGTA 46595 ||| || Sbjct 1638854 CGACTA 1638859 >seq3 Length=3655701 Score = 116 bits (128), Expect = 3e-21 Identities = 162/226 (72%), Gaps = 2/226 (1%) Strand=Plus/Plus Query 131008 CCACCGCGCAAACGCAGCACTAAATGTATGGTCGACTCCTTTTGAATGTTGTAATCGCTC 131067 ||||| |||| || ||||| | || | |||||||||||| || |||||||| ||| | Sbjct 2673564 CCACCACGCAGGCGGAGCACCAGGTGGAGGGTCGACTCCTTCTGGATGTTGTAGTCGGAC 2673623 Query 131068 ATGGTGCGCGAGTCCTCTAGCTGCTTGCCTGCGTAGATTAAACGCTGCTGGTCCGGAGGG 131127 | || || ||||| ||||||||||| || |||| | ||||||||||| || ||| Sbjct 2673624 AGCGTCCGGCCATCCTCGAGCTGCTTGCCGGCAAAGATCAGGCGCTGCTGGTCAGGCGGG 2673683 Query 131128 ATTCCTTCTTTGTCGGTGATTTTCTGCTTGAGCTGCTCCACAGTATCGGTGGACTCGATG 131187 || || || ||||| ||| || ||||| | || | || || | |||||||| Sbjct 2673684 ATGCCCTCCTTGTCCTGGATCTTGCTCTTGACGTTGTCGATGGTGTCCGAGGACTCGACC 2673743 Query 131188 TCGACGGTAACGGTTTTACCGGTTAGAGTTTTTACGAAAATCTGCA 131233 |||| ||| | ||| || || || || || || |||||| ||||| Sbjct 2673744 TCGAGGGTGATGGTCTTGCCCGTGAGGGTCTTCACGAAA--CTGCA 2673787 >seq10 Length=919426 Score = 111 bits (122), Expect = 1e-19 Identities = 162/229 (71%), Gaps = 0/229 (0%) Strand=Plus/Minus Query 131007 GCCACCGCGCAAACGCAGCACTAAATGTATGGTCGACTCCTTTTGAATGTTGTAATCGCT 131066 |||||| || | |||||||| | || | || |||||| || |||||||| ||| Sbjct 645315 GCCACCACGGAGCCGCAGCACCAGGTGGAGCGTGCTCTCCTTCTGGATGTTGTAGTCGGA 645256 Query 131067 CATGGTGCGCGAGTCCTCTAGCTGCTTGCCTGCGTAGATTAAACGCTGCTGGTCCGGAGG 131126 || ||| || |||||| ||||||||||||||| |||| | ||||||||||| || || Sbjct 645255 CAGGGTCCGGCCGTCCTCCAGCTGCTTGCCTGCGAAGATCAGGCGCTGCTGGTCTGGGGG 645196 Query 131127 GATTCCTTCTTTGTCGGTGATTTTCTGCTTGAGCTGCTCCACAGTATCGGTGGACTCGAT 131186 || || || ||||| ||| || ||||| | || | || |||| ||||| | Sbjct 645195 AATGCCCTCCTTGTCCTGGATCTTGGACTTGACATTGTCGATGGTGTCGGACGACTCCAC 645136 Query 131187 GTCGACGGTAACGGTTTTACCGGTTAGAGTTTTTACGAAAATCTGCATG 131235 || | ||| | || || || || || || || ||||| ||||||||| Sbjct 645135 CTCAAGGGTGATAGTCTTGCCCGTGAGGGTCTTGACGAAGATCTGCATG 645087 Lambda K H 0.634 0.408 0.912 Gapped Lambda K H 0.625 0.410 0.780 Effective search space used: 472012646595840