Alignment viewer

BLASTN 2.6.0+ Reference: Stephen F. Altschul, Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Database: Saccharomyces_20cerevisiae_20[genbank_20GCA_5F000766495.2_202014-10- 12] 389 sequences; 11,590,380 total letters Query= NC_003389.1 Length=146454 Score E Sequences producing significant alignments: (Bits) Value seq269 194 2e-44 seq136 176 4e-39 seq162 138 1e-27 >seq269 Length=22145 Score = 194 bits (214), Expect = 2e-44 Identities = 422/629 (67%), Gaps = 2/629 (0%) Strand=Plus/Minus Query 38006 CATCTTTTTCTACGCGTTCCATAAATAAATCAGGTACCCATAATGCAGTAAATAAATCTT 38065 ||| || ||| || || | ||| || | |||||| | ||||| |||| || |||||| Sbjct 10147 CATTTTCTTCGACACGCTTCATGAAAAGATCAGGAATCCATAGAGCAGGGAACAAATCTC 10088 Query 38066 TGGTTCTATATTCTTCGTTCCCTACATTCTTTCTTAAGCTTAGAAAATCGAATATATCAG 38125 | | | | ||| || || || ||||| || | | |||||| || ||||||| Sbjct 10087 TTGCACGAATTTCCTCTTTACCGTGGTTCTTCCTAATATCAATAAAATCAAAGATATCAG 10028 Query 38126 CGTGCCATGGTTCTATATAAATCGACATTGCTCCTGGTCTTTTATCACCTCCCTGATTAA 38185 | ||||||||||| | |||| | | || || |||||||||| ||| ||||| | || Sbjct 10027 CATGCCATGGTTCCAGGTAAAGGGCAAACGCACCAGGTCTTTTATTACCACCCTGGTCAA 9968 Query 38186 TATATCTAACTGTGTTATTATATATTCTTAACATAGGTACTATACCATTAGATTTGCCGT 38245 ||| | | |||||||| | | | | |||||| | || ||| ||||| | |||| Sbjct 9967 CATAACGGGCGGTGTTATTGAAAACACGAATCATAGGAATTAAACCGTTAGAAGTACCGT 9908 Query 38246 TTATACCGGATATATAAGTACCCTTAGCTCGTATATTACTTATCGACAATCCTATTCCAC 38305 || |||| | || |||| ||| | | || || ||| || | || || |||| Sbjct 9907 TTGTACCAGCAATGTAAGAACCAGTTGAACGAATGTTATGGATATGTAGACCAATACCAC 9848 Query 38306 CTGCATGTTTTGATATTAAAGCGCATTGTTTTAACGTATCAAATATACCTTCTATACTGT 38365 | ||| ||| || || ||||| |||| || || || || | || || || ||| || Sbjct 9847 CAGCAGTTTTGGAAATCAAAGCACATTCCTTCAAGGTGTCGTAAATCCCCTCGATAGAGT 9788 Query 38366 CATCCTTTATTGGTAGTATAAAACAACTAGACATTTGAGGCAATGGAGTTCCGGCGTTAA 38425 | ||||| || | | | |||||| ||||||||||| |||||| ||||| || | Sbjct 9787 CCTCCTTCATGGCAACCAAGAAACAAGAGGACATTTGAGGTTTTGGAGTACCGGCATTGA 9728 Query 38426 ATAATGTAGGAGATGCATGTATGAACCATTTTTCCGACAAATAATTATATGTTTCTATTA 38485 | || || ||||| || || | || ||||| |||| | ||||| ||||||| Sbjct 9727 ACAACGTTGGAGAGGCGTGAGTAAAATATTTTAGAGACATCAAGTTATACGTTTCTAAAG 9668 Query 38486 CATCATCTATATCACCGCCGTGAATACCTAGAGATACCCTCATTAGCATATGTTGTGGTC 38545 || | || ||||| | ||||| || ||||| | || ||||| | | ||||||||| | Sbjct 9667 CAGCCTCGATATCTCTACCGTGGATGCCTAGTGCGACTCTCATAATTAAATGTTGTGGAC 9608 Query 38546 TTTCTATTATTTTATTATTCTGTTTAAGTAGATACGATTTTTCTAATGTTTTTATTCCAA 38605 ||| | || | || | | |||| ||| || ||| || ||||| | |||| Sbjct 9607 GTTCGGCCACTTGACCGTTGATTCTTAGTAAATAAGAACGTTCCAAAGTTTTAAAACCAA 9548 Query 38606 AATAACTATA-TGAATAATCTCTATCGTA 38633 ||||||| || |||| ||||||| || || Sbjct 9547 AATAACTGTACTGAA-AATCTCTGTCATA 9520 Score = 183 bits (202), Expect = 3e-41 Identities = 549/839 (65%), Gaps = 15/839 (2%) Strand=Plus/Minus Query 36790 TCAACTGTGAATTTTATAGGATTTGATGCCGATTTTGTTCTCATATAATACATTCCAGTT 36849 |||| ||||||| || | | ||||| | || ||||||| ||| ||||| || || Sbjct 11381 TCAATAGTGAATTGAATTGCAGCAGATGCAGCTTGGGTTCTCAAATAGTACATACCGGTC 11322 Query 36850 TTTAGCCCCAATCTCCATCCGTAAAAATGCATACTGGTCAGTTTTGCGTATGTAGGATTA 36909 || | ||| |||||||||||||||||||||| || ||||| | || || | Sbjct 11321 TTCAATCCCTTCTTCCATCCGTAAAAATGCATACTTGTTAGTTTACCCATAGTTGGGGCA 11262 Query 36910 TCTATATATATTGTCATTGATTGACTTTGATCTATAAACGCACCTCTGTCGGCTGCCATT 36969 || ||| ||| || ||| |||||| ||| | || | ||||||||||| Sbjct 11261 CGTAAGAATAAATTCAAAGAATGAGATTGATCAATATAGACAGAACGATCGGCTGCCATG 11202 Query 36970 TCTAAAATACATTTTTGAGATATCTCCCAAACCGTTTTATATATTTGTTTTATATTATCT 37029 | | ||| ||||| || || |||||||| || || |||| | || | | | | Sbjct 11201 TTAATGATAGTCTTTTGTGAAATTTCCCAAACAGTCTTGTATAAGTCCTTCAATTCTTGT 11142 Query 37030 GGTAAATCT---AAATGTTGAATAGAACCATTATGT---AACACAATTGTATTCTTT-AT 37082 || | || | || |||||| || ||||| ||| | || | || | ||| || Sbjct 11141 GGAACATTTGGTAAGCCTTGAATGGAGCCATTTTGTGTAATCAGA----TACTGTTTCAT 11086 Query 37083 GTCATTATTCCACATATTTCTACTTATTAGTTCTCTCAATAGGTGTGGATTTACAACCTG 37142 | | || ||| ||| | | | | || | || || || || || |||||||| Sbjct 11085 ACCCTCATCCCAAATACCTAAATCAACCAAGTCACGCAGTAAGTAAGGGTTCACAACCTG 11026 Query 37143 AAAATCTCCAGATAATACTCTTCTTGTATATATATTGCTAGTATATGGTTCAATGGACTC 37202 || || || ||||| || | | | || |||||| ||| |||||| | | || Sbjct 11025 GAATTCACCGGATAAGACACGACGGGAGTACATATTGGAAGTGACTGGTTCGAAACATTC 10966 Query 37203 ATTATTACTTAATATTTGAGATGTAGATGCAGTAGGCATTGGTGCTATTAGAAGGCTATT 37262 ||||| || |||||||| ||||| |||||||||||||||||||| || | | ||| Sbjct 10965 ATTATAACCCAATATTTGGGATGTGGATGCAGTAGGCATTGGTGCCATGGTCAAGGAATT 10906 Query 37263 TCTCAATCCATG-TGTATTAATTCTCTTTTTTAGTTCATTCCAATCCCATAAATCTGTTG 37321 ||| | ||||| | || | ||| || || || | | ||||||||| | | || | Sbjct 10905 TCTAATACCATGCTTCATGATATCTTTTCTTAAGGT-GTCCCAATCCCA-CATGCCGTAG 10848 Query 37322 GGTTCTT-TAACCACATATCATATTGTAGAATACCTTTACTCGCTGGAGAATCGTTATAT 37380 |||| || |||||||||| | ||||| ||||||| | || ||||| | | | Sbjct 10847 GGTTTTTGGTCCCACATATCAAACTGTAGTATACCTTGAGAAGCAGGAGATCCTTGGAAA 10788 Query 37381 GTCTCATAAGGTCCGTAAATTTTAGCTAGTTCGCAACTAGATTCTAGTGCGGCATAATAT 37440 || || || || |||| | | ||||||||| ||| || ||| | | ||||| ||| Sbjct 10787 GTTTCGTATGGACCGTCCTTCTGAGCTAGTTCACAAGAAGCTTCCATGGAGGCATGATAA 10728 Query 37441 ATTGTTTCGAAAATTTGTATATTTAATATTTTTGCTTCTTCGCTATCGAATGCATAGCCC 37500 || ||||| || ||||| ||||||| | ||||| || ||| |||| | | Sbjct 10727 ATAGTTTCAAAGATTTGGATATTTAGCAAACGGGCTTCCTCAGAATCAAATGGCAAACGT 10668 Query 37501 AATAATATAAACACATCAGCCAAACCCTGAACACCGATACCTATGGGTCTATGTTTCTTA 37560 || | || || ||||| | ||| |||||||| | | | || ||||||||| || || Sbjct 10667 AACAGCATGAAAGTGTCAGCGAGACCTTGAACACCCAAAGCAATTGGTCTATGTCTCATA 10608 Query 37561 TTAGATCTAGACGATTCTATCACTGGATAATAATTGATATCTATTATTTTATTTAGATT 37619 || ||| | || |||| || || || ||||| ||| || | | | |||| ||| Sbjct 10607 TTGGATTTTCTCGCTTCTTCAACAGGGTAGTAATTACGATCAATGACTCTGTTTAAATT 10549 >seq136 Length=106972 Score = 176 bits (194), Expect = 4e-39 Identities = 1077/1698 (63%), Gaps = 74/1698 (4%) Strand=Plus/Minus Query 36982 TTTTGAGATATCTCCCAAACCGTTTTATATATTTGTTTTATATTATCTGGTAAATCT--- 37038 |||||||| || ||||| || ||||| |||| || || | | | ||| | || | Sbjct 78865 TTTTGAGAGATTTCCCAGACGGTTTTGTATAATTCCTTCAATTCTTGTGGCACATTTGGT 78806 Query 37039 AAATGTTGAATAGAACCATTATGTAACACAATTG--TATTCTTT-ATGTCATTATTCCAC 37095 || ||||||||||||||| ||| |||| |||| ||| || || || ||| Sbjct 78805 AAGCCTTGAATAGAACCATTTTGTGT---AATTAGATATTGTTTCATACTATCATCCCAA 78749 Query 37096 ATATTTCTACTT--ATTAGTTCTCTCAATAGGTGTGGATTTACAACCTGAAAATCTCCAG 37153 ||| | | | | || || | | || | |||||| ||||| || || || |||| Sbjct 78748 ATAC--CCAGGTCGACTAAATCACGTAGTAAATATGGATTAACAACTTGGAATTCACCAG 78691 Query 37154 ATAATACTCTTCTTGTATATATATTGCTAGTATATGGTTCAATGGACTCATTATTACTTA 37213 | | || | | | || || || || |||||| | | | ||||||| || | Sbjct 78690 ACAGGACACGACGAGAGTACATGTTTGAGGTCACTGGTTCGAAGCATTCATTATAACCAA 78631 Query 37214 ATATTTGAGATGTAGATGCAGTAGGCATTGGTGCTATTAG--AAGGCTATTTCTCAATCC 37271 ||||| || || || || || ||||||||||| || || || | ||||| || || Sbjct 78630 GAATTTGGGAAGTTGAGGCGGTTGGCATTGGTGCCAT-AGTCAAAGAG-TTTCTTAACCC 78573 Query 37272 ATGTGTATTAATTCTCTTTTTTA--GTTCATTCCAATCCCATAAATCTGTTGGGTT--CT 37327 |||| || ||| |||| ||| ||| | ||||||||| | | ||| || | Sbjct 78572 ATGTTTAACAATGTCCTTTCTTAAGGTT--TCCCAATCCCACATGCCAAATGGTTTAGCG 78515 Query 37328 TTAACCACATATCATATTGTAGAATACCTTTACTCGCTGGAGAATCGTTATATGTCTCAT 37387 || ||||||||| |||||| |||||| ||| ||||||||| | | | | || || Sbjct 78514 TT--CCACATATCGAATTGTAAAATACCCTTAGAAGCTGGAGAACCTTCAAAAGTAGAAT 78457 Query 37388 AAGGTCCGTAAATTTTAGCTAGTTCGCAACTAGATTCTAGTGCGGCATAATATATTGTTT 37447 | || | ||| || | ||| || | ||| || | |||| || || |||| Sbjct 78456 ATTTACCTTCTTTTTGGGCCAATTCACAGGAGGCTTCAAGAGTAGCATGGTAAATAGTTT 78397 Query 37448 CGAAAATTTGTATATTTAATATTTTTGCTTCTTCGCTATCGAATGCATAGCC-CAATAAT 37506 |||| |||||| | |||| ||| |||||||| | || || | ||| | ||| | Sbjct 78396 CGAAGATTTGTTTGTTTAGAGTTTGAGCTTCTTC-CGATTCAAAGGGTAGACGCAACATC 78338 Query 37507 ATAAACACATCAGCCAAACCCTGAACACCGATACCTATGGGTCTATGTTTCTTATTAGA- 37565 ||| | ||| ||||||||||| ||||| | | | || |||||||| ||| |||| || Sbjct 78337 ATATAAGTATCGGCCAAACCCTGGACACCAAGAGCAATAGGTCTATGCTTCATATTTGAA 78278 Query 37566 --TCTAGACGATTCTATCACTGGATAATAATTGATATCTATTATTTTATTTAGATTTTTA 37623 ||||| | || |||||||| ||||| || || | | | || | | || | Sbjct 78277 TTTCTAGCC---TCGGGAACTGGATAGTAATTACGGTCGATAACTCTGTTCA-AGTTGTG 78222 Query 37624 ACTAGT-ATTTTCGTAAT-TATTCTCAACTTGTCGAACATAAATACGTT--ATTATTAAC 37679 | || | | ||| | ||| | | | | || ||||| || || || | | ||| | Sbjct 78221 AGTAATGACTTTAGCAATCTCGTGTAATCTC-TCGAAATTA--TAGCTTGCAGTTTTACC 78165 Query 37680 AAATTTA-----CTCAACGAA-------------ATAGATCCTAGATTACATACAGCAAC 37721 | || ||||||||| ||||| ||| |||||| || ||| Sbjct 78164 ATCTTCTGAAACCTCAACGAATGCTGGTAGGGCAATAGAAGCTAAATTACAAACTGCAGT 78105 Query 37722 TTCATCTTCGTTACTAAATTGTATAATCTCTGTACAGAGATTACTCGATCTAATTGTTCC 37781 |||||| | | ||| | || || ||| | |||| ||| | || || || Sbjct 78104 TTCATCCGGGGAGGAATATTCGACGATTTCACAACATAAATTAGATGATTTGATAGTACC 78045 Query 37782 CAAATTGCTTTGATTGGATTTTTTATTACAAGAATCTTTATATAGTATATACGGCGTTCC 37841 || || ||| || | || | ||||| | || ||||| | || | || || || Sbjct 78044 TAAGTTCTGTTGGTTTGTCTTCCTGTTACATGCGTCCTTATAAACCATGAAAGGTGTACC 77985 Query 37842 TTGTTCTATTTGAGCCTCGATAATAGATTTCCATAAACTTCTTGCTTTAACGATCGCTTT 37901 | |||| | || || | | ||| | | ||| || || ||||||| | | ||| Sbjct 77984 TGTTTCTGTCTGTGCTTGCAAAATGGCATACCACAACTTTTGGGCTTTAA---TTGTTTT 77928 Query 37902 A--AtttttttCTTTGTTTCGTATTCTATATATAATTTGTTAAAATCATTCCCCCATACA 37959 | | |||| | |||||| |||||| || | ||| |||| ||||| ||| Sbjct 77927 ACCACGACCTTCTCT--TTCGTAACGAGTATATAGTTCTTCAAATTCATCACCCCACACA 77870 Query 37960 TCGCATAATCCTGGA-CATTCGCA-TGGAC-ACATTAGAGACCATTCTTCATCTTTTTCT 38016 || ||| ||||| || ||| ||| | | | | || ||| |||| | || Sbjct 77869 TCATCTAAACCTGGGGCA---GCACTGGGCGAAAACAAAGTCCAAGGCCCATCCTCTTGA 77813 Query 38017 ACGCGTTCCATAAATAAATCAGGTACCCATAATGCAGTAAATAAATCTTTGGTTCTATAT 38076 || |||| ||| || | |||||| | ||||| |||| || |||||| | | | | | Sbjct 77812 ACACGTTTCATGAAAAGATCAGGGATCCATAGAGCAGGGAACAAATCTCTTGCACGAATT 77753 Query 38077 TCTTCGTTCCCTACATTCTTTCTTAAGCTTAGAAAATCGAATATATCAGCGTGCCATGGT 38136 ||||| || || | ||||| | | ||| ||||| ||||| || |||||||| Sbjct 77752 TCTTCCTTACCATGTGTTTTTCTGATATCGACAAAGTCGAAGATATCTGCATGCCATGGC 77693 Query 38137 TCTATATAAATCGACATTGCTCCTGGTCTTTTATCACCTCCCTGATTAATATATCTAACT 38196 || | ||| | | || || ||||| || | ||| ||||| | | ||| | | Sbjct 77692 TCCAAGAAAAGGGCGAAAGCACCAGGTCTCTTGTTACCACCCTGGTCCACATAACGGGCA 77633 Query 38197 GTGTTATTATATATTCTTAACATAGGTACTATACCATTAGATTTGCCGTTTATACCGGAT 38256 || ||||| | | | | |||||| | | || || || | ||||| |||| | Sbjct 77632 GTATTATTGAAAACACGAATCATAGGAATCAACCCGTTTGAAGTACCGTTGGTACCAGCG 77573 Query 38257 ATATAAGTACCCTTAGCTCGTATATTACTTATCGACAATCCTATTCCACCTGCATGTTTT 38316 ||||||| ||| | | || || || | || | || | ||||||||| ||| Sbjct 77572 ATATAAGAACCTGTGGAACGGATGTTGTTGATATGAAGACCAACACCACCTGCAGTTTTG 77513 Query 38317 GATATTAAAGCGCATTGTTTTAACGTATCAAATATACCTTCTATACTGTCATCCTTTATT 38376 || || | ||| |||| ||| || |||||| | |||||||| ||| ||||||||| || Sbjct 77512 GAAATCATAGCACATTCTTTCAAAGTATCATAAATACCTTCGATAGAGTCATCCTTCATG 77453 Query 38377 GGTAGTATAAAACAACTAGACATTTGAGGCAATGGAGTTCCGGCGTTAAATAATGTAGGA 38436 | | || ||||| |||||||||||| ||| || || ||||| ||||| || || Sbjct 77452 GCAATTAAGAAACATGAAGACATTTGAGGATGTGGCGTACCAGCGTTGAATAAAGTTGGG 77393 Query 38437 GATGCATGTATGAACCATTTTTCCGACAAATAATTATATGTTTCTATTACATCATCTATA 38496 || || || |||| || || ||||| ||||||| || | | ||| || ||| Sbjct 77392 GAAGCGTGAGTGAAGTATCTTAACGACATCAAATTATAAGTCTTCAGCACAGATTCGATA 77333 Query 38497 TCACCGCCGTGAATACCTAGAGATACCCTCATTAGCATATGTTGTGGTCTTTCTATTATT 38556 || | || || |||||||| | || | ||||| || ||| || || | |||| | | Sbjct 77332 TCGCTACCATGGATACCTAGCGCCACACGCATTACCAAATGCTGAGGACGTTCTGCCACT 77273 Query 38557 TTATTATTCTGTTTAAGTAGATACGATTTTTCTAATGTTTTTATTCCAAAATAACTATA- 38615 | | ||| || | || | ||||| || | ||| || | ||| ||||| |||| Sbjct 77272 TCACCGTTCAGTCTTAGCAAGTACGAACGCTCCAGTGTCTTGAATCCGAAATACGTATAC 77213 Query 38616 TGAATAATCTCTATCGTA 38633 || | |||| |||||||| Sbjct 77212 TGGA-AATCCCTATCGTA 77196 >seq162 Length=69633 Score = 138 bits (152), Expect = 1e-27 Identities = 398/602 (66%), Gaps = 24/602 (4%) Strand=Plus/Plus Query 11621 AACATTAAACACGCAAAATCACAATGTAAACCTTCGTCTCTACTTATTAGTTCATTAGAA 11680 |||| || || ||||| || || |||||||||||||||| || | ||| || ||| Sbjct 31601 AACAACAAGCATGCAAAGTCGGTGTGCAAACCTTCGTCTCTACAGATCAATTCGTTGGAA 31660 Query 11681 AATGTTAATCCGGGCATCAATCCTCGtttttttATCCAAAATATAGCAGCAAATGAACCA 11740 || ||||| |||||||||| |||| ||||| | ||| || || | ||||| ||||| Sbjct 31661 AAGGTTAAACCGGGCATCATACCTCTCTTTTTCAACCAGAAAATGGAGGCAAAGGAACCG 31720 Query 11741 GAAAAGAATATTCCCTCCACAGCTGCAAATGCTACTACTCTTTCTCCATATA---CCT-T 11796 || || || | || || | | ||||| || |||| |||||| ||| | | | | Sbjct 31721 GAGAAAAAGACACCTTCAATGGAGGCAAAGGCAACTAGTCTTTCACCAAACAAGGCGTCA 31780 Query 11797 GTTGCTAGATATCCATTTTCTGGCCCAATCAGCTTTCTTTTTTACGCATTCC----ATTG 11852 | || || |||||| || ||||| || || ||| | || ||| | || | Sbjct 31781 GCGTCTTGA-ATCCATCTTAAAGCCCATTCGGCCTTC----TCACCGATTTCTGGGATGG 31835 Query 11853 TTTCTATAGCGTTAAATA--AATGCATTTTTTCTATATTATCTCTTACATATGTATCTAT 11910 | | || || || || | ||| || ||||| || || | | || ||||| || Sbjct 31836 TGTGAATGGCATTGAACAAGAATTCA--CTTTCTTTAGGGTCCTTGATGTAAGTATCGAT 31893 Query 11911 TAATAAACTATACATTTCTGAATGAATATTTTCCATAGCTATTTGAAATCCATAGAAACA 11970 || || || |||| || ||||||||||| || ||||| || || ||||||| Sbjct 31894 CAACAAGGAGTAAGTTTCAGAGTGAATATTTTCAATCATGATTTGGAAACCGTAGAAACT 31953 Query 11971 TCGTGCCTCTGAACACTGTACATCCACATAAAATCTTTCCGCTAAATTCTCATTTACAAT 12030 |||||||| | || || || | || |||| | || || ||||| ||||| Sbjct 31954 CTTTGCCTCTGGAATTTGGACTTCGGTGGAGAAGTTTTCAACCAAGTTTTCATTAACAAT 32013 Query 12031 ACCATCACTAGATGCAAAAAATGCTAGTA-TATGTTTTATAAAGTATTTTTCGTCTTTAG 12089 ||| ||| || || ||||| || || | | || || || || | |||| ||| | Sbjct 32014 ACCGTCAGAAGCGGCGAAAAAGGCAAGAACTCTG-GAAATGAAAAATCTCTCGT-TTTCG 32071 Query 12090 TTAAT----TTATCCCAATCATCTAAATCTTTTGATAAATCTACTTCTTCAACGGTCCAA 12145 || || || | ||| |||| | ||| || || ||||| | ||||||| ||||||| Sbjct 32072 TTCATTCTGTTGTTCCAGTCATGGATATCCTTAGACAAATCAATTTCTTCAGCGGTCCAG 32131 Query 12146 AAACTTGCCACTGATTGTTTATACATTTTCCAGATATCATGATACTTAATAGGGAAAATA 12205 ||| || | | | || || || |||||| ||||| ||||| |||||||||| | Sbjct 32132 AAAGAAGCTTCGGCACGCTTGTAGGCTTGCCAGATTTCATGGTACTTGATAGGGAAAAGA 32191 Query 12206 AC 12207 || Sbjct 32192 AC 32193 Lambda K H 0.634 0.408 0.912 Gapped Lambda K H 0.625 0.410 0.780 Effective search space used: 468522521798160