Alignment viewer

BLASTN 2.6.0+ Reference: Stephen F. Altschul, Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Database: Saccharomyces_20cerevisiae_20[genbank_20GCA_5F001738465.1_202016-09- 21] 91 sequences; 12,091,764 total letters Query= NC_003389.1 Length=146454 Score E Sequences producing significant alignments: (Bits) Value seq5 194 2e-44 seq9 172 5e-38 seq10 143 2e-29 >seq5 Length=561356 Score = 194 bits (214), Expect = 2e-44 Identities = 422/629 (67%), Gaps = 2/629 (0%) Strand=Plus/Minus Query 38006 CATCTTTTTCTACGCGTTCCATAAATAAATCAGGTACCCATAATGCAGTAAATAAATCTT 38065 ||| || ||| || || | ||| || | |||||| | ||||| |||| || |||||| Sbjct 296428 CATTTTCTTCGACACGCTTCATGAAAAGATCAGGAATCCATAGAGCAGGGAACAAATCTC 296369 Query 38066 TGGTTCTATATTCTTCGTTCCCTACATTCTTTCTTAAGCTTAGAAAATCGAATATATCAG 38125 | | | | ||| || || || ||||| || | | |||||| || ||||||| Sbjct 296368 TTGCACGAATTTCCTCTTTACCGTGGTTCTTCCTAATATCAATAAAATCAAAGATATCAG 296309 Query 38126 CGTGCCATGGTTCTATATAAATCGACATTGCTCCTGGTCTTTTATCACCTCCCTGATTAA 38185 | ||||||||||| | |||| | | || || |||||||||| ||| ||||| | || Sbjct 296308 CATGCCATGGTTCCAGGTAAAGGGCAAACGCACCAGGTCTTTTATTACCACCCTGGTCAA 296249 Query 38186 TATATCTAACTGTGTTATTATATATTCTTAACATAGGTACTATACCATTAGATTTGCCGT 38245 ||| | | |||||||| | | | | |||||| | || ||| ||||| | |||| Sbjct 296248 CATAACGGGCGGTGTTATTGAAAACACGAATCATAGGAATTAAACCGTTAGAAGTACCGT 296189 Query 38246 TTATACCGGATATATAAGTACCCTTAGCTCGTATATTACTTATCGACAATCCTATTCCAC 38305 || |||| | || |||| ||| | | || || ||| || | || || |||| Sbjct 296188 TTGTACCAGCAATGTAAGAACCAGTTGAACGAATGTTATGGATATGTAGACCAATACCAC 296129 Query 38306 CTGCATGTTTTGATATTAAAGCGCATTGTTTTAACGTATCAAATATACCTTCTATACTGT 38365 | ||| ||| || || ||||| |||| || || || || | || || || ||| || Sbjct 296128 CAGCAGTTTTGGAAATCAAAGCACATTCCTTCAAGGTGTCGTAAATCCCCTCGATAGAGT 296069 Query 38366 CATCCTTTATTGGTAGTATAAAACAACTAGACATTTGAGGCAATGGAGTTCCGGCGTTAA 38425 | ||||| || | | | |||||| ||||||||||| |||||| ||||| || | Sbjct 296068 CCTCCTTCATGGCAACCAAGAAACAAGAGGACATTTGAGGTTTTGGAGTACCGGCATTGA 296009 Query 38426 ATAATGTAGGAGATGCATGTATGAACCATTTTTCCGACAAATAATTATATGTTTCTATTA 38485 | || || ||||| || || | || ||||| |||| | ||||| ||||||| Sbjct 296008 ACAACGTTGGAGAGGCGTGAGTAAAATATTTTAGAGACATCAAGTTATACGTTTCTAAAG 295949 Query 38486 CATCATCTATATCACCGCCGTGAATACCTAGAGATACCCTCATTAGCATATGTTGTGGTC 38545 || | || ||||| | ||||| || ||||| | || ||||| | | ||||||||| | Sbjct 295948 CAGCCTCGATATCTCTACCGTGGATGCCTAGTGCGACTCTCATAATTAAATGTTGTGGAC 295889 Query 38546 TTTCTATTATTTTATTATTCTGTTTAAGTAGATACGATTTTTCTAATGTTTTTATTCCAA 38605 ||| | || | || | | |||| ||| || ||| || ||||| | |||| Sbjct 295888 GTTCGGCCACTTGACCGTTGATTCTTAGTAAATAAGAACGTTCCAAAGTTTTAAAACCAA 295829 Query 38606 AATAACTATA-TGAATAATCTCTATCGTA 38633 ||||||| || |||| ||||||| || || Sbjct 295828 AATAACTGTACTGAA-AATCTCTGTCATA 295801 Score = 183 bits (202), Expect = 3e-41 Identities = 549/839 (65%), Gaps = 15/839 (2%) Strand=Plus/Minus Query 36790 TCAACTGTGAATTTTATAGGATTTGATGCCGATTTTGTTCTCATATAATACATTCCAGTT 36849 |||| ||||||| || | | ||||| | || ||||||| ||| ||||| || || Sbjct 297662 TCAATAGTGAATTGAATTGCAGCAGATGCAGCTTGGGTTCTCAAATAGTACATACCGGTC 297603 Query 36850 TTTAGCCCCAATCTCCATCCGTAAAAATGCATACTGGTCAGTTTTGCGTATGTAGGATTA 36909 || | ||| |||||||||||||||||||||| || ||||| | || || | Sbjct 297602 TTCAATCCCTTCTTCCATCCGTAAAAATGCATACTTGTTAGTTTACCCATAGTTGGGGCA 297543 Query 36910 TCTATATATATTGTCATTGATTGACTTTGATCTATAAACGCACCTCTGTCGGCTGCCATT 36969 || ||| ||| || ||| |||||| ||| | || | ||||||||||| Sbjct 297542 CGTAAGAATAAATTCAAAGAATGAGATTGATCAATATAGACAGAACGATCGGCTGCCATG 297483 Query 36970 TCTAAAATACATTTTTGAGATATCTCCCAAACCGTTTTATATATTTGTTTTATATTATCT 37029 | | ||| ||||| || || |||||||| || || |||| | || | | | | Sbjct 297482 TTAATGATAGTCTTTTGTGAAATTTCCCAAACAGTCTTGTATAAGTCCTTCAATTCTTGT 297423 Query 37030 GGTAAATCT---AAATGTTGAATAGAACCATTATGT---AACACAATTGTATTCTTT-AT 37082 || | || | || |||||| || ||||| ||| | || | || | ||| || Sbjct 297422 GGAACATTTGGTAAGCCTTGAATGGAGCCATTTTGTGTAATCAGA----TACTGTTTCAT 297367 Query 37083 GTCATTATTCCACATATTTCTACTTATTAGTTCTCTCAATAGGTGTGGATTTACAACCTG 37142 | | || ||| ||| | | | | || | || || || || || |||||||| Sbjct 297366 ACCCTCATCCCAAATACCTAAATCAACCAAGTCACGCAGTAAGTAAGGGTTCACAACCTG 297307 Query 37143 AAAATCTCCAGATAATACTCTTCTTGTATATATATTGCTAGTATATGGTTCAATGGACTC 37202 || || || ||||| || | | | || |||||| ||| |||||| | | || Sbjct 297306 GAATTCACCGGATAAGACACGACGGGAGTACATATTGGAAGTGACTGGTTCGAAACATTC 297247 Query 37203 ATTATTACTTAATATTTGAGATGTAGATGCAGTAGGCATTGGTGCTATTAGAAGGCTATT 37262 ||||| || |||||||| ||||| |||||||||||||||||||| || | | ||| Sbjct 297246 ATTATAACCCAATATTTGGGATGTGGATGCAGTAGGCATTGGTGCCATGGTCAAGGAATT 297187 Query 37263 TCTCAATCCATG-TGTATTAATTCTCTTTTTTAGTTCATTCCAATCCCATAAATCTGTTG 37321 ||| | ||||| | || | ||| || || || | | ||||||||| | | || | Sbjct 297186 TCTAATACCATGCTTCATGATATCTTTTCTTAAGGT-GTCCCAATCCCA-CATGCCGTAG 297129 Query 37322 GGTTCTT-TAACCACATATCATATTGTAGAATACCTTTACTCGCTGGAGAATCGTTATAT 37380 |||| || |||||||||| | ||||| ||||||| | || ||||| | | | Sbjct 297128 GGTTTTTGGTCCCACATATCAAACTGTAGTATACCTTGAGAAGCAGGAGATCCTTGGAAA 297069 Query 37381 GTCTCATAAGGTCCGTAAATTTTAGCTAGTTCGCAACTAGATTCTAGTGCGGCATAATAT 37440 || || || || |||| | | ||||||||| ||| || ||| | | ||||| ||| Sbjct 297068 GTTTCGTATGGACCGTCCTTCTGAGCTAGTTCACAAGAAGCTTCCATGGAGGCATGATAA 297009 Query 37441 ATTGTTTCGAAAATTTGTATATTTAATATTTTTGCTTCTTCGCTATCGAATGCATAGCCC 37500 || ||||| || ||||| ||||||| | ||||| || ||| |||| | | Sbjct 297008 ATAGTTTCAAAGATTTGGATATTTAGCAAACGGGCTTCCTCAGAATCAAATGGCAAACGT 296949 Query 37501 AATAATATAAACACATCAGCCAAACCCTGAACACCGATACCTATGGGTCTATGTTTCTTA 37560 || | || || ||||| | ||| |||||||| | | | || ||||||||| || || Sbjct 296948 AACAGCATGAAAGTGTCAGCGAGACCTTGAACACCCAAAGCAATTGGTCTATGTCTCATA 296889 Query 37561 TTAGATCTAGACGATTCTATCACTGGATAATAATTGATATCTATTATTTTATTTAGATT 37619 || ||| | || |||| || || || ||||| ||| || | | | |||| ||| Sbjct 296888 TTGGATTTTCTCGCTTCTTCAACAGGGTAGTAATTACGATCAATGACTCTGTTTAAATT 296830 >seq9 Length=434108 Score = 172 bits (190), Expect = 5e-38 Identities = 1076/1698 (63%), Gaps = 74/1698 (4%) Strand=Plus/Plus Query 36982 TTTTGAGATATCTCCCAAACCGTTTTATATATTTGTTTTATATTATCTGGTAAATCT--- 37038 |||||||| || ||||| || ||||| |||| || || | | | ||| | || | Sbjct 237405 TTTTGAGAGATTTCCCAGACGGTTTTGTATAGTTCCTTCAATTCTTGTGGCACATTTGGT 237464 Query 37039 AAATGTTGAATAGAACCATTATGTAACACAATTG--TATTCTTT-ATGTCATTATTCCAC 37095 || ||||||||||||||| ||| |||| |||| ||| || || || ||| Sbjct 237465 AAGCCTTGAATAGAACCATTTTGTGT---AATTAGATATTGTTTCATACTATCATCCCAA 237521 Query 37096 ATATTTCTACTT--ATTAGTTCTCTCAATAGGTGTGGATTTACAACCTGAAAATCTCCAG 37153 ||| | | | | || || | | || | |||||| ||||| || || || |||| Sbjct 237522 ATAC--CCAGGTCGACTAAATCACGTAGTAAATATGGATTAACAACTTGGAATTCACCAG 237579 Query 37154 ATAATACTCTTCTTGTATATATATTGCTAGTATATGGTTCAATGGACTCATTATTACTTA 37213 | | || | | | || || || || |||||| | | | ||||||| || | Sbjct 237580 ACAGGACACGACGAGAGTACATGTTTGAGGTCACTGGTTCGAAGCATTCATTATAACCAA 237639 Query 37214 ATATTTGAGATGTAGATGCAGTAGGCATTGGTGCTATTAG--AAGGCTATTTCTCAATCC 37271 ||||| || || || || || ||||||||||| || || || | ||||| || || Sbjct 237640 GAATTTGGGAAGTTGAGGCGGTTGGCATTGGTGCCAT-AGTCAAAGAG-TTTCTTAACCC 237697 Query 37272 ATGTGTATTAATTCTCTTTTTTA--GTTCATTCCAATCCCATAAATCTGTTGGGTT--CT 37327 |||| || ||| |||| ||| ||| | ||||||||| | | ||| || | Sbjct 237698 ATGTTTAACAATGTCCTTTCTTAAGGTT--TCCCAATCCCACATGCCAAATGGTTTAGCG 237755 Query 37328 TTAACCACATATCATATTGTAGAATACCTTTACTCGCTGGAGAATCGTTATATGTCTCAT 37387 || ||||||||| |||||| |||||| ||| ||||||||| | | | | || || Sbjct 237756 TT--CCACATATCGAATTGTAAAATACCCTTAGAAGCTGGAGAACCTTCAAAAGTAGAAT 237813 Query 37388 AAGGTCCGTAAATTTTAGCTAGTTCGCAACTAGATTCTAGTGCGGCATAATATATTGTTT 37447 | || | ||| || | ||| || | ||| || | |||| || || |||| Sbjct 237814 ATTTACCTTCTTTTTGGGCCAATTCACAGGAGGCTTCAAGAGTAGCATGGTAAATAGTTT 237873 Query 37448 CGAAAATTTGTATATTTAATATTTTTGCTTCTTCGCTATCGAATGCATAGCC-CAATAAT 37506 |||| |||||| | |||| ||| ||| |||| | || || | ||| | ||| | Sbjct 237874 CGAAGATTTGTTTGTTTAGAGTTTGAGCTACTTC-CGATTCAAAGGGTAGACGCAACATC 237932 Query 37507 ATAAACACATCAGCCAAACCCTGAACACCGATACCTATGGGTCTATGTTTCTTATTAGA- 37565 ||| | ||| ||||||||||| ||||| | | | || |||||||| ||| |||| || Sbjct 237933 ATATAAGTATCGGCCAAACCCTGGACACCAAGAGCAATAGGTCTATGCTTCATATTTGAA 237992 Query 37566 --TCTAGACGATTCTATCACTGGATAATAATTGATATCTATTATTTTATTTAGATTTTTA 37623 ||||| | || |||||||| ||||| || || | | | || | | || | Sbjct 237993 TTTCTAGCC---TCGGGAACTGGATAGTAATTACGGTCGATAACTCTGTTCA-AGTTGTG 238048 Query 37624 ACTAGT-ATTTTCGTAAT-TATTCTCAACTTGTCGAACATAAATACGTT--ATTATTAAC 37679 | || | | ||| | ||| | | | | || ||||| || || || | | ||| | Sbjct 238049 AGTAATGACTTTAGCAATCTCGTGTAATCTC-TCGAAATTA--TAGCTTGCAGTTTTACC 238105 Query 37680 AAATTTA-----CTCAACGAA-------------ATAGATCCTAGATTACATACAGCAAC 37721 | || ||||||||| ||||| ||| |||||| || ||| Sbjct 238106 ATCTTCTGAAACCTCAACGAATGCTGGTAGGGCAATAGAAGCTAAATTACAAACTGCAGT 238165 Query 37722 TTCATCTTCGTTACTAAATTGTATAATCTCTGTACAGAGATTACTCGATCTAATTGTTCC 37781 |||||| | | ||| | || || ||| | |||| ||| | || || || Sbjct 238166 TTCATCCGGGGAGGAATATTCGACGATTTCACAACATAAATTAGATGATTTGATAGTACC 238225 Query 37782 CAAATTGCTTTGATTGGATTTTTTATTACAAGAATCTTTATATAGTATATACGGCGTTCC 37841 || || ||| || | || | ||||| | || ||||| | || | || || || Sbjct 238226 TAAGTTCTGTTGGTTTGTCTTCCTGTTACATGCGTCCTTATAAACCATGAAAGGTGTACC 238285 Query 37842 TTGTTCTATTTGAGCCTCGATAATAGATTTCCATAAACTTCTTGCTTTAACGATCGCTTT 37901 | |||| | || || | | ||| | | ||| || || ||||||| | | ||| Sbjct 238286 TGTTTCTGTCTGTGCTTGCAAAATGGCATACCACAACTTTTGGGCTTTAA---TTGTTTT 238342 Query 37902 A--AtttttttCTTTGTTTCGTATTCTATATATAATTTGTTAAAATCATTCCCCCATACA 37959 | | |||| | |||||| |||||| || | ||| |||| ||||| ||| Sbjct 238343 ACCACGACCTTCTCT--TTCGTAACGAGTATATAGTTCTTCAAATTCATCACCCCACACA 238400 Query 37960 TCGCATAATCCTGGA-CATTCGCA-TGGAC-ACATTAGAGACCATTCTTCATCTTTTTCT 38016 || ||| ||||| || ||| ||| | | | | || ||| |||| | || Sbjct 238401 TCATCTAAACCTGGGGCA---GCACTGGGCGAAAACAAAGTCCAAGGCCCATCCTCTTGA 238457 Query 38017 ACGCGTTCCATAAATAAATCAGGTACCCATAATGCAGTAAATAAATCTTTGGTTCTATAT 38076 || |||| ||| || | |||||| | ||||| |||| || |||||| | | | | | Sbjct 238458 ACACGTTTCATGAAAAGATCAGGGATCCATAGAGCAGGGAACAAATCTCTTGCACGAATT 238517 Query 38077 TCTTCGTTCCCTACATTCTTTCTTAAGCTTAGAAAATCGAATATATCAGCGTGCCATGGT 38136 ||||| || || | ||||| | | ||| ||||| ||||| || |||||||| Sbjct 238518 TCTTCCTTACCATGTGTTTTTCTGATATCGACAAAGTCGAAGATATCTGCATGCCATGGC 238577 Query 38137 TCTATATAAATCGACATTGCTCCTGGTCTTTTATCACCTCCCTGATTAATATATCTAACT 38196 || | ||| | | || || ||||| || | ||| ||||| | | ||| | | Sbjct 238578 TCCAAGAAAAGGGCGAAAGCACCAGGTCTCTTGTTACCACCCTGGTCCACATAACGGGCA 238637 Query 38197 GTGTTATTATATATTCTTAACATAGGTACTATACCATTAGATTTGCCGTTTATACCGGAT 38256 || ||||| | | | | |||||| | | || || || | ||||| |||| | Sbjct 238638 GTATTATTGAAAACACGAATCATAGGAATCAACCCGTTTGAAGTACCGTTGGTACCAGCG 238697 Query 38257 ATATAAGTACCCTTAGCTCGTATATTACTTATCGACAATCCTATTCCACCTGCATGTTTT 38316 ||||||| ||| | | || || || | || | || | ||||||||| ||| Sbjct 238698 ATATAAGAACCTGTGGAACGGATGTTGTTGATATGAAGACCAACACCACCTGCAGTTTTG 238757 Query 38317 GATATTAAAGCGCATTGTTTTAACGTATCAAATATACCTTCTATACTGTCATCCTTTATT 38376 || || | ||| |||| ||| || |||||| | |||||||| ||| ||||||||| || Sbjct 238758 GAAATCATAGCACATTCTTTCAAAGTATCATAAATACCTTCGATAGAGTCATCCTTCATG 238817 Query 38377 GGTAGTATAAAACAACTAGACATTTGAGGCAATGGAGTTCCGGCGTTAAATAATGTAGGA 38436 | | || ||||| |||||||||||| ||| || || ||||| ||||| || || Sbjct 238818 GCAATTAAGAAACATGAAGACATTTGAGGATGTGGCGTACCAGCGTTGAATAAAGTTGGG 238877 Query 38437 GATGCATGTATGAACCATTTTTCCGACAAATAATTATATGTTTCTATTACATCATCTATA 38496 || || || |||| || || ||||| ||||||| || | | ||| || ||| Sbjct 238878 GAAGCGTGAGTGAAGTATCTTAACGACATCAAATTATAAGTCTTCAGCACAGATTCGATA 238937 Query 38497 TCACCGCCGTGAATACCTAGAGATACCCTCATTAGCATATGTTGTGGTCTTTCTATTATT 38556 || | || || |||||||| | || | ||||| || ||| || || | |||| | | Sbjct 238938 TCGCTACCATGGATACCTAGCGCCACACGCATTACCAAATGCTGAGGACGTTCTGCCACT 238997 Query 38557 TTATTATTCTGTTTAAGTAGATACGATTTTTCTAATGTTTTTATTCCAAAATAACTATA- 38615 | | ||| || | || | ||||| || | ||| || | ||| ||||| |||| Sbjct 238998 TCACCGTTCAGTCTTAGCAAGTACGAACGCTCCAGTGTCTTGAATCCGAAATACGTATAC 239057 Query 38616 TGAATAATCTCTATCGTA 38633 || | |||| |||||||| Sbjct 239058 TGGA-AATCCCTATCGTA 239074 >seq10 Length=742111 Score = 143 bits (158), Expect = 2e-29 Identities = 399/602 (66%), Gaps = 24/602 (4%) Strand=Plus/Minus Query 11621 AACATTAAACACGCAAAATCACAATGTAAACCTTCGTCTCTACTTATTAGTTCATTAGAA 11680 |||| || || ||||| || || |||||||||||||||| || | ||| || ||| Sbjct 397599 AACAACAAGCATGCAAAGTCGGTGTGCAAACCTTCGTCTCTACAGATCAATTCGTTGGAA 397540 Query 11681 AATGTTAATCCGGGCATCAATCCTCGtttttttATCCAAAATATAGCAGCAAATGAACCA 11740 || ||||| |||||||||| |||| ||||| | ||| || || | ||||| ||||| Sbjct 397539 AAGGTTAAACCGGGCATCATACCTCTCTTTTTCAACCAGAAAATGGAGGCAAAGGAACCG 397480 Query 11741 GAAAAGAATATTCCCTCCACAGCTGCAAATGCTACTACTCTTTCTCCATATA---CCT-T 11796 || || || | || || | | ||||| || |||| |||||| ||| | | | | Sbjct 397479 GAGAAAAAGACACCTTCAATGGAGGCAAAGGCAACTAGTCTTTCACCAAACAAGGCGTCA 397420 Query 11797 GTTGCTAGATATCCATTTTCTGGCCCAATCAGCTTTCTTTTTTACGCATTCC----ATTG 11852 | || || |||||| || ||||| || || ||| | || ||| | || | Sbjct 397419 GCGTCTTGA-ATCCATCTTAAAGCCCATTCGGCCTTC----TCACCGATTTCTGGGATGG 397365 Query 11853 TTTCTATAGCGTTAAATA--AATGCATTTTTTCTATATTATCTCTTACATATGTATCTAT 11910 | | || || || || | ||| || ||||| || || | | || ||||| || Sbjct 397364 TGTGAATGGCATTGAACAAGAATTCA--CTTTCTTTAGGGTCCTTGATGTAAGTATCGAT 397307 Query 11911 TAATAAACTATACATTTCTGAATGAATATTTTCCATAGCTATTTGAAATCCATAGAAACA 11970 || || || |||| || ||||||||||| || ||||| || || ||||||| Sbjct 397306 CAACAAGGAGTAAGTTTCAGAGTGAATATTTTCAATCATGATTTGGAAACCGTAGAAACT 397247 Query 11971 TCGTGCCTCTGAACACTGTACATCCACATAAAATCTTTCCGCTAAATTCTCATTTACAAT 12030 |||||||| | || || || |||| |||| | || || ||||| ||||| Sbjct 397246 CTTTGCCTCTGGAATTTGGACTTCGGTGGAAAAGTTTTCAACCAAGTTTTCATTAACAAT 397187 Query 12031 ACCATCACTAGATGCAAAAAATGCTAGTA-TATGTTTTATAAAGTATTTTTCGTCTTTAG 12089 ||| ||| || || ||||| || || | | || || || || | |||| ||| | Sbjct 397186 ACCGTCAGAAGCGGCGAAAAAGGCAAGAACTCTG-GAAATGAAAAATCTCTCGT-TTTCG 397129 Query 12090 TTAAT----TTATCCCAATCATCTAAATCTTTTGATAAATCTACTTCTTCAACGGTCCAA 12145 || || || | ||| |||| | ||| || || ||||| | ||||||| ||||||| Sbjct 397128 TTCATTCTGTTGTTCCAGTCATGGATATCCTTAGACAAATCAATTTCTTCAGCGGTCCAG 397069 Query 12146 AAACTTGCCACTGATTGTTTATACATTTTCCAGATATCATGATACTTAATAGGGAAAATA 12205 ||| || | | | || || || |||||| ||||| ||||| |||||||||| | Sbjct 397068 AAAGAAGCTTCGGCACGCTTGTAGGCTTGCCAGATTTCATGGTACTTGATAGGGAAAAGA 397009 Query 12206 AC 12207 || Sbjct 397008 AC 397007 Lambda K H 0.634 0.408 0.912 Gapped Lambda K H 0.625 0.410 0.780 Effective search space used: 468524267053040