Alignment viewer
BLASTN 2.6.0+
Reference: Stephen F. Altschul, Thomas L. Madden, Alejandro A.
Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J.
Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of
protein database search programs", Nucleic Acids Res. 25:3389-3402.
Database: Ustilago_20maydis_20[genbank_20GCA_5F001736215.1_202016-09-20]
2,695 sequences; 20,132,962 total letters
Query= NC_003102.1
Length=139342
Score E
Sequences producing significant alignments: (Bits) Value
seq2275 450 1e-121
seq2563 196 4e-45
seq2363 105 6e-18
seq1585 91.5 1e-13
seq999 89.7 5e-13
>seq2275
Length=103831
Score = 450 bits (498), Expect = 1e-121
Identities = 1242/1881 (66%), Gaps = 44/1881 (2%)
Strand=Plus/Plus
Query 22115 GTAAACTGTATGGCGTTTGCGGCCGGTTTGGTGCGAAGATAATACATGCCCGTCTTCAGA 22174
||||| || || ||||| | ||||| ||||| || ||||| || | |||||||| ||
Sbjct 42741 GTAAATTGGATCGCGTTCGACGCCGGCTTGGTCCGCAGATAGTATGTACCCGTCTTGAGT 42800
Query 22175 CCCATCTTCCACGCGTAAAAATGTATCGACGTCAATTTTCCATAACTCGGCAAGTCCATG 22234
||| | ||||| ||||||| || ||||| || | | |||| | |||| | |
Sbjct 42801 CCCTTTTTCCAGCCGTAAAAGTGCATCGAGGTGAGCTGTCCAAACGTCGGTGCCGAAAGG 42860
Query 22235 TACACATTGAACGACTGACTCTGATCGATAAACGCTCCTCGATCGGCGGCCATATTTATC 22294
| | |||| |||||| ||| ||||||||||||||| || || ||||| | |
Sbjct 42861 TGAATGTTGAGCGACTGCGACTGGCAGATAAACGCTCCTCGGTCCGCAGCCATGT----C 42916
Query 22295 AATGTACGCATC--GAC--ATTTCCCATACGGTCTTGTACAGTTGCCTGAT-CTCTAACG 22349
||| ||| | | | || ||||| || ||||||||||| | | |||| | | | |
Sbjct 42917 GATGATCGCCTTCTGGCTGATCTCCCAGACCGTCTTGTACAGCTTCTTGATGCGGTCA-G 42975
Query 22350 GTATCGATTCGATCCTCTGGACGGACCCGTTATTGTGTATGATCAAATTTTTAATCGTAT 22409
| || | | |||||| || ||| | ||||| || || ||||| |
Sbjct 42976 GAATGCCTGGCACGTTCTGGATCGATCCGCCGTGCGCGATGATACGGTTCTTCATCGTGT 43035
Query 22410 CGTTCCACAAACCGATGGCGGTCAAATCTTTGACCAGATGACGG-TTCACCACTTGAAAC 22468
||||||||| || | || || | || | |||| |||||||| || |||
Sbjct 43036 CGTTCCACAGGCCCTGCTCCACCAGCTCGCGCAACA-ACCACGGGTTCACCACCTGGAAC 43094
Query 22469 TCGCCGGACAAGACGCGGCGTTGGTAAATGTTGGATGTGAACGGTTCGAAAGATTCATTG 22528
|| || | | || || || ||| |||||||| || | || ||||| | || ||
Sbjct 43095 TCACCCGCGAGCACACGCCGAGAGTAGATGTTGGACGTATAAGGCTCGAAGCACTCGTTA 43154
Query 22529 TTTCCCAGTATCTGAGCGGTCGAAGCGGTCGGCATCGGCGCCAACAGTAGCGAGTTTCTC 22588
||||| |||||| |||||||||||||||||||| |||| ||| |||||||| |
Sbjct 43155 AAGCCCAGGATCTGACTCGTCGAAGCGGTCGGCATCGGTGCCAGCAGCAGCGAGTTGCGA 43214
Query 22589 AAGCCGTGCTTTGAAATTTTACTCTTGAGCATCGACCAGTCCCACAGATCGGACGGCGTC 22648
| |||||||| | || || ||||||| || |||||||||||| || ||||||||
Sbjct 43215 AGCCCGTGCTTGGCGATCTTGGCCTTGAGCTCCGCCCAGTCCCACAGGTCCGACGGCGTG 43274
Query 22649 ACATTGTCCCACATATCGTATTGCAA--CACGCCGCGACTCGCCGGGCTGCCTTCGTACG 22706
|| ||||||| ||||| ||||| ||| | |||| || || ||| ||||||
Sbjct 43275 AC---CCCCCACATGTCGTACTGCAACTCACCCTGCGA--AGCGGGCGAGCCGGCGTACG 43329
Query 22707 TTTCGTACGGACCATATATCGCGGCAAGTTCGCA-GCTCGCCTCCAACGCGCCGTAATAT 22765
| ||||| || | | | || || ||||| | ||| ||||| ||| ||| ||
Sbjct 43330 TCTCGTAGCTGCCGTGCTGCTCAGCCAGCTCGCACGATCG-CTCCAGTGCGGCGTGGTAG 43388
Query 22766 ATAGTCTCGAAGATTTTCTTGTTCAATTCGGCG-GCGGCGACGCTTTCGTACGGCATA-C 22823
|| ||||| ||||| | | |||| | ||| || || || | || || ||||| |
Sbjct 43389 ATGGTCTCAAAGATCTGCACGTTC-AGTCGCCGAGCCTCGGCCGACTCAAAC-GCATACC 43446
Query 22824 GCATCATCACGAACGCGTCCGCCATACCCTGAACGCCTATACCGATCGGTCGGTTTTTCA 22883
||| |||| |||||| ||||| | |||||||| || || |||||||| |||| ||
Sbjct 43447 CCATGATCATGAACGCATCCGCAAGGCCCTGAACACCGATCCCGATCGGACGGTGACGCA 43506
Query 22884 GATTCGACACTTTTGCCTCGGGCAAAGGATACCAATTCGAATCGATTGTTCTGTTCAAGT 22943
|| ||| ||||| | ||||| | || ||||| | |||||| ||
Sbjct 43507 TGTTGGACTTGCGCGCCTCCTCGACCGGATAGTAGTTAATGTCGATGATCTTGTTCAGGT 43566
Query 22944 TTCTGGTCACAATTTTCGTTATNNNNNNNNNNNNNTCAAAGTCGTAAACGCC-----CGA 22998
| || | || | | | |||||||| ||| | |
Sbjct 43567 TCTTGCAGATGGCCTTGGCAACGCTGTGCAGACGCTTAAAGTCGT--ACGTCTTGGTCAC 43624
Query 22999 TTGTACAACAAACTTGTTGACGGCGATCGAGGCAAGATTGCATACGGCCACCTCGTCTTT 23058
| | ||| ||| || | ||||||||||| || ||||| || |||||||||||
Sbjct 43625 TGGGTCAATGAACGTGGGCAGTGCGATCGAGGCGAGGTTGCACACTGCCACCTCGTC--G 43682
Query 23059 GGA-CG-TGTACTCTACAATTTCGGTACATAGATTGCTACACTTTATCGTGCCCAAATTG 23116
||| || ||||||| | || |||||||| | ||| ||||| |||||||| | ||
Sbjct 43683 GGAGCGCTGTACTCGATGATCTCGGTACACAAGTTGGAGCACTTGATCGTGCCGAGGTTC 43742
Query 23117 CTCTGGTTGCTTTTTCGATTGCACGAATCCTTGTAGAGCATGTACGGCGTGCCCGTCTCG 23176
|||||||||| || || ||| | |||||||| || ||| | || |||||||||||
Sbjct 43743 TTCTGGTTGCTCTTGCGGTTGGCGGCGTCCTTGTACAGGATGAAAGGGTTGCCCGTCTCG 43802
Query 23177 ACCTGCGACTCGATGATCGCTCGCCACATAGTCTGAGCGTGAACACGTCGCACGTACCTG 23236
||| ||||||| ||| || ||| | |||| || | || ||| | |
Sbjct 43803 GTCTGTGACTCGAGGATGGCGTACCAGAGCTTCTGCGCTTTGACGGTGCGCTTCGAGCGA 43862
Query 23237 CCTTCTTCCTCGTACTTTTGGTACA-ACGCGTCAAATTCGGCACCGTAACAGTCGGCCAG 23295
|| || ||||||| | ||| | ||| ||||| ||| ||||||| |||||||| ||
Sbjct 43863 CCCTCGGCCTCGTAGCGCTCGTAGAGGCGC-TCAAACTCGTCACCGTAGCAGTCGGCTAG 43921
Query 23296 GCCGGGCGATTGCATCGGACACATGAGACTCCACATTGAGTCATTTTCAACGCGTCTCAT 23355
|| || | | | || |||||||| ||| || | || || | ||||
Sbjct 43922 ACCAGGGCACTCGTTGGGGCACATGAGCGACCAGTCGCCGTTCTGCTCGACCCTCTTCAT 43981
Query 23356 AAAAAGATCCGGTATCCATAGAGCGTAGAACAGTTCGCGGGCGCGCACCTCCTCTTTGCC 23415
|| ||||| || | ||| || || ||||| || || || || ||||||||||| || ||
Sbjct 43982 GAAGAGATCAGGCACCCAGAGTGCATAGAAGAGCTCACGCGCACGCACCTCCTCCTTTCC 44041
Query 23416 GGTGTTCTTTTTCAAATCCAAAAAGTCCAACACGTCCGCATGCCACGGCTCGAGGTAAAC 23475
| |||||| || || | || || || ||||||| ||||| || || | |||||
Sbjct 44042 GTGGTTCTTGCGCAGGTCGAGGAACTCAAAGACGTCCGAGTGCCATGGTTCAATGTAAAT 44101
Query 23476 GGCAAACGCGCCCGGTCTCTTATTGCCGCCCTGATCGACGTAACGGGCCGTGTTGTTGTA 23535
||||| || || || | ||| ||||| ||||| |||||||| || || ||||||||| |
Sbjct 44102 AGCAAAGGCACCGGGACGCTTGTTGCCACCCTGGTCGACGTAGCGCGCAGTGTTGTTGAA 44161
Query 23536 CACTCTTAGCATCGGCACGAGACCGTTGGACGCGCCGCCTACGCCCGCCACACTCGAACC 23595
|| | ||||| ||||| | |||||| | ||| ||| || | ||| ||
Sbjct 44162 GACGCGGAGCATGGGCACAATGCCGTTGCTGACACCGTTGGTGCCGGCAATGTACGAGCC 44221
Query 23596 TTTTGCTCGAACGTTGTGAACGTGGAAACCTATGCCTCCGCCCGATTTCGATATCATCGC 23655
| | || | | || | || || || ||||| ||| ||| || || ||||| ||
Sbjct 44222 GGTGCCACGGATGCAATGGATGTTGAGGCCGATGCCACCGGCCGTCTTGGAGATCATGGC 44281
Query 23656 GCATTGTTTCAGAGTGTCGTAGATGCCCTCGATGCTGTCCTCTTTGGTCGCGACCAAGAA 23715
|| || || || ||||||||||| ||||| ||| || || | | || | |||
Sbjct 44282 ACAAGTCTTGAGGGTATCGTAGATGCCGTCGATCGAGTCATCCTTCATGGTAACAAGGAA 44341
Query 23716 ACACGAAGACATTTGCGCTTTGGGCGTAGCGGCCGAGAACAGTGTCGGGCTGGCGTGCGT 23775
||||| || | |||| | || ||| |||| ||| || || || |||||| ||
Sbjct 44342 GCACGACGAGAGCTGCGGGTGCGGGGTACCGGCGTTGAAGAGCGTAGGCGAGGCGTGGGT 44401
Query 23776 AAAGTATCCATTCGACATTAGATTGTACGTGTCGATGGCGGCATCAATGTCCTCTCCGTG 23835
|||||| | |||||| || |||||||| ||||||||| || ||||||| |||||
Sbjct 44402 AAAGTACTTTTGCGACATAAGGTTGTACGTCTCGATGGCGCTCTCGATGTCCTGGCCGTG 44461
Query 23836 TATACCGATGGCGACGCGCATCATCATGTATTGAGGTCTCT---CGACCGTGACGCCGTT 23892
|| |||| ||||| ||||| | ||||| || || | || ||||| | ||||||
Sbjct 44462 GATGCCGACAGCGACTCGCATGAGCATGTGCTGGGGACGCTCTGCGACCTT---GCCGTT 44518
Query 23893 AATTTTGAACAGATATGAACGTTGCATCGTTTTAAATCCAAAATAGTCATAGTTAAAGTC 23952
|| || || ||| ||||| | | ||| || || || ||| ||| |||| |||||
Sbjct 44519 GATGCGGAGCAAATAGGAACGCTCGAGCGTCTTGAAGCCGAAAAAGTTGTAGTGGAAGTC 44578
Query 23953 ACGATCGTGCACTATGGCCGA 23973
||| |||| | ||||||||
Sbjct 44579 ACGTTCGTAGATGATGGCCGA 44599
>seq2563
Length=74346
Score = 196 bits (216), Expect = 4e-45
Identities = 563/855 (66%), Gaps = 47/855 (5%)
Strand=Plus/Minus
Query 57570 ATTTGGAACATGTACAAAAAGGCAGAAGCATCGTTTTGGACCGTCGAAGAAGTAGACCTG 57629
|||||| | |||||||| ||||| || || || || ||||||| || || | |||||
Sbjct 34835 ATTTGGCAAATGTACAAGAAGGCCGAGGCTTCCTTCTGGACCGCAGAGGAGATCGACCTT 34776
Query 57630 TCTAAGGATCTGAGCGATTGGGATTCC---CTCAAAGAATCTGAGAGAAATTTTATTAAA 57686
|| ||||| || ||| ||||| | || || || |||| | | |||||
Sbjct 34775 TCCAAGGACCTCCACGACTGGGACAACAAGCTGAACGACAATGAGCGCCACTTTATCTCG 34716
Query 57687 CACGTGTTGGCGTTTTTCGCCGCATCCGATGGTATTGTTAACGAGAATTTGGTCGAAAGA 57746
|| ||| | ||||| ||||| || || |||||||| || |||||||| | || || |
Sbjct 34715 CATGTGCTTGCGTTCTTCGCTGCGTCGGATGGTATCGTCAACGAGAACCTCGTTGAGCG- 34657
Query 57747 TTTTCACAA--GAAGTGCAAGTGACGGAGGCGAGATGCTTTTACGGATTTCAAGTGGCCA 57804
||||| ||||| || | | ||||| | ||||| ||||| || || | |
Sbjct 34656 -TTTCAGTTCGGAAGTTCAGGCCGCCGAGGCTCGTTGCTTCTACGGTTTCCAGATCATGA 34598
Query 57805 TTGAAAACGTGCATTCAGAAATGTATTCGCTACTCATCGATACGTACGTGAAGGACAACG 57864
| || ||| | || || || | || ||||| |||||||| || ||| | || |
Sbjct 34597 TGGAGAACATCCACTCGGAGACCTACTCGCTGCTCATCGACACTTACATCCGTGA----G 34542
Query 57865 AC-GAGAAACGTAGA---CTGTTCAACGCGATCGAAACAATGCCCGCGATCGCCAAG--- 57917
| ||| | ||| | ||||| |||| |||| || || || | ||| ||||
Sbjct 34541 CCTGAGCAGCGTGAATATCTGTTTGACGCCGTCGAGACCATCCCGG---TCGTCAAGCAG 34485
Query 57918 AAGGCAGAGTGGTCAATCAAATGGATAAAAAGCGCCACG---GCCACGTTCGCCGAGAGG 57974
||||| || ||| | | |||||| | ||| || | ||||| ||||||||| |
Sbjct 34484 AAGGCCGACTGGGCTCTTCGATGGATCA---GCGACAAGCGTGCCACTTTCGCCGAGCGT 34428
Query 57975 CTAATCGCGTTCGCCGCCGTCGAAGGTATATTCTTTTCGGGCAGCTTTGCGGCAATATTT 58034
|| ||||||| ||||||||||| || || || || |||||| || || | || ||
Sbjct 34427 CTGGTCGCGTTTGCCGCCGTCGAGGGCATCTTTTTCTCGGGCTCATTCGCCTCGATCTTC 34368
Query 58035 TGGATGAAGAAACGCGGCCTGATGCCCGGCCTGACGTTTAGCAACGAGTTGATATCGCGC 58094
||| | ||||| ||||| || |||||||| || ||||| ||||||| | || |||||
Sbjct 34367 TGGCTCAAGAAGCGCGGTCTCATGCCCGGTCTCACGTTCTCCAACGAGCTCATCTCGCGT 34308
Query 58095 GACGAGGGTCTCCATACTGATTTTGCGTGCCTAATGTACTCTCACATTGTCAATAAACCG 58154
||||||||||| || || || ||||| ||||| | | | ||| ||| | |
Sbjct 34307 GACGAGGGTCTTCACACGGACTTTGCCTGCCTTCTCTTCAGCCAC---CTCAGGA----G 34255
Query 58155 ACGCGGGATCGCA-------TCTTGGACATTATCACCGAGGCGGTCGCGATCGAAAATGA 58207
|||| | | || | | | || ||||| ||||| || | ||||| || |
Sbjct 34254 GCGCGCGCACCCAGACACGGTGCTCAAGATCATCACTGAGGCCGTGGAAATCGAGAAGGC 34195
Query 58208 GTTTGTCACCGAAGCCCTACCGGTGGCCATGCTCGGTATGAACAATGATTTGATGTCAAA 58267
||| | |||| | | || || || | |||||||||||| | | |||| |
Sbjct 34194 GTTCCTGTGCGAATCAATCCCCGTCGCGCTCATCGGTATGAACAGCAAGCTCATGTGCCA 34135
Query 58268 GTACATTGAGTTTGTTGCCGACAGACTTTTGCTCGAACTGATTGGAGAGC--GT-CATTA 58324
|||||||||| ||| ||||| | ||||| | | | | ||| | | || || ||
Sbjct 34134 ATACATTGAGTATGTCGCCGA---TCGCTTGCTGGTATCGCTCGGAAACCCAGTGCACTA 34078
Query 58325 CAACACGAAAAATCCGTTTGATTTTATGAATCTGATCTCGCTCGAGGGCAAGACAAACTT 58384
| | | | | ||||||||| ||||||| || ||| | || |||||||| |||||
Sbjct 34077 CGGCTCCACATGTCCGTTTGACTTTATGACCAACATTTCGTTGGAAGGCAAGACCAACTT 34018
Query 58385 TTTCGaaaaaaaaGT 58399
|| || ||||| ||
Sbjct 34017 CTTTGAGAAAAAGGT 34003
>seq2363
Length=266066
Score = 105 bits (116), Expect = 6e-18
Identities = 151/213 (71%), Gaps = 0/213 (0%)
Strand=Plus/Minus
Query 31193 ATGCAAATTTTCGTAAAGACTCTGACAGGCAAAACGATCACCGTCGACGTTGAACCGTCC 31252
||||| || || || ||||| || |||||||| || ||||| |||| || || | ||
Sbjct 64085 ATGCAGATCTTTGTCAAGACGCTCACAGGCAAGACCATCACTCTCGAAGTCGAGTCTTCG 64026
Query 31253 GACAGTGTCGAAACGGTCAAACAGAGAATAGCGGACAAGGAAGGCGTGCCGCCCGACCAG 31312
|||| |||| | ||||| | || |||||||||||| | || || || |||
Sbjct 64025 GACACCATCGACAACGTCAAGGCCAAGATCCAAGACAAGGAAGGCATTCCTCCTGATCAG 63966
Query 31313 CAGCGTCTAATATTCGCTGGCAAACAGTTGGAAGACTCTATGACTATGAGCGATTATAAC 31372
|||||||| || || || ||||| ||| | ||||| | ||| || || || |||
Sbjct 63965 CAGCGTCTTATCTTTGCCGGCAAGCAGCTCGAAGATGGTCGCACTCTGTCGGACTACAAC 63906
Query 31373 ATTCAAAAAGAATCGACTCTTCATCTGGTACTT 31405
||||| || ||||| |||||||| |||| |||
Sbjct 63905 ATTCAGAAGGAATCCACTCTTCACTTGGTCCTT 63873
Score = 98.7 bits (108), Expect = 9e-16
Identities = 159/229 (69%), Gaps = 0/229 (0%)
Strand=Plus/Minus
Query 31193 ATGCAAATTTTCGTAAAGACTCTGACAGGCAAAACGATCACCGTCGACGTTGAACCGTCC 31252
||||| || || || ||||| || || ||||| || || || |||| || || | ||
Sbjct 64313 ATGCAGATCTTTGTTAAGACGCTCACGGGCAAGACCATTACTCTCGAAGTCGAGTCCTCG 64254
Query 31253 GACAGTGTCGAAACGGTCAAACAGAGAATAGCGGACAAGGAAGGCGTGCCGCCCGACCAG 31312
|||| |||| | ||||| | || |||||||||||| | || || || |||
Sbjct 64253 GACACCATCGACAACGTCAAGGCCAAGATCCAAGACAAGGAAGGCATTCCTCCTGATCAG 64194
Query 31313 CAGCGTCTAATATTCGCTGGCAAACAGTTGGAAGACTCTATGACTATGAGCGATTATAAC 31372
|||||||| || |||||||| || ||| | |||||| || | ||| || |||
Sbjct 64193 CAGCGTCTCATCTTCGCTGGTAAGCAGCTCGAAGACGGCCGAACGCTCTCCGACTACAAC 64134
Query 31373 ATTCAAAAAGAATCGACTCTTCATCTGGTACTTAGACTGAGGGGCGGTA 31421
||||| ||||| ||||| ||||| || ||| | |||| | || ||||
Sbjct 64133 ATTCAGAAAGAGTCGACGCTTCACCTTGTATTGCGACTCCGCGGTGGTA 64085
>seq1585
Length=212
Score = 91.5 bits (100), Expect = 1e-13
Identities = 142/203 (70%), Gaps = 0/203 (0%)
Strand=Plus/Plus
Query 31199 ATTTTCGTAAAGACTCTGACAGGCAAAACGATCACCGTCGACGTTGAACCGTCCGACAGT 31258
|| || || ||||||||||| ||||| ||||||||| | || || || | |||||||
Sbjct 1 ATCTTTGTCAAGACTCTGACCGGCAAGACGATCACCCTTGAGGTGGAGTCTTCCGACACA 60
Query 31259 GTCGAAACGGTCAAACAGAGAATAGCGGACAAGGAAGGCGTGCCGCCCGACCAGCAGCGT 31318
|||| | ||||| | || ||||||||| || | || || ||||||||||||
Sbjct 61 ATCGACAATGTCAAGGCCAAGATCCAGGACAAGGAGGGTATCCCCCCTGACCAGCAGCGT 120
Query 31319 CTAATATTCGCTGGCAAACAGTTGGAAGACTCTATGACTATGAGCGATTATAACATTCAA 31378
|| || || ||||| || ||| | |||||| || | || || ||||| ||
Sbjct 121 CTCATCTTTGCTGGTAAGCAGCTTGAAGACGGCCGCACGCTCTCGGACTACAACATCCAG 180
Query 31379 AAAGAATCGACTCTTCATCTGGT 31401
|| || ||||| ||||| || ||
Sbjct 181 AAGGAGTCGACACTTCACCTTGT 203
>seq999
Length=205769
Score = 89.7 bits (98), Expect = 5e-13
Identities = 115/159 (72%), Gaps = 0/159 (0%)
Strand=Plus/Plus
Query 31190 AACATGCAAATTTTCGTAAAGACTCTGACAGGCAAAACGATCACCGTCGACGTTGAACCG 31249
|| ||||| || || || ||||| || || || || || ||||| |||| || || |
Sbjct 80228 AAGATGCAGATCTTTGTCAAGACCCTCACGGGTAAGACCATCACTCTCGAGGTGGAGTCT 80287
Query 31250 TCCGACAGTGTCGAAACGGTCAAACAGAGAATAGCGGACAAGGAAGGCGTGCCGCCCGAC 31309
|| |||| |||| | ||||| | || ||||||||| ||| | |||||||||
Sbjct 80288 TCGGACACCATCGACAATGTCAAGGCCAAGATCCAGGACAAGGAGGGCATTCCGCCCGAC 80347
Query 31310 CAGCAGCGTCTAATATTCGCTGGCAAACAGTTGGAAGAC 31348
||||||||||| || |||||||| || ||| | || |||
Sbjct 80348 CAGCAGCGTCTGATCTTCGCTGGTAAGCAGCTCGAGGAC 80386
Lambda K H
0.634 0.408 0.912
Gapped
Lambda K H
0.625 0.410 0.780
Effective search space used: 445751383244400
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