Alignment viewer

BLASTN 2.6.0+ Reference: Stephen F. Altschul, Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Database: Gerris_20buenoi_20[genbank_20GCA_5F001010745.1_202015-05-21] 20,259 sequences; 1,000,161,732 total letters Query= NC_001993.1 Length=236120 Score E Sequences producing significant alignments: (Bits) Value seq9819 159 5e-34 seq297 138 2e-27 seq1037 107 3e-18 seq472 86.0 9e-12 >seq9819 Length=195331 Score = 159 bits (176), Expect = 5e-34 Identities = 395/592 (67%), Gaps = 37/592 (6%) Strand=Plus/Minus Query 205449 ATGTGCAATCATATAAGTCAATAAACTATAACTAGCAACATTAAATGGTACACCTAAACC 205508 ||| || || || | || ||||| |||||| || | ||||| ||||| || || || Sbjct 12283 ATGAGCTATTATGTGTGTTAATAATGCATAACTTGCTATGTTAAAAGGTACTCCCAACCC 12224 Query 205509 CATATCAGCTGATCTTTGATACATTTGACATGTAAGAT----------ATTTATTATT-- 205556 |||||| || ||||||||| | | ||||| | | | ||||||| || Sbjct 12223 CATATCTGCAGATCTTTGAAATAAATGACAAGACAATTCTCTTTTACCATTTATTTTTGC 12164 Query 205557 AATTACATGAAATTGTACAAAACAATGACAAGGTGGTATAACCATATTTTCAACATCTAT 205616 | |||||| ||| ||| | | |||||||||||| || | |||| || | ||| | Sbjct 12163 ACTTACATCAAAATGTGCTATACAATGACAAGGAGGAAGTGCCATTTTAGAATTATCCTT 12104 Query 205617 AGGATTCCAGGCTGTTAATATTATATTTCTAGATTCAGGtttattttttattttatttat 205676 || ||||| ||| |||||| || ||||| ||||||||||| ||| | | || Sbjct 12103 TGGGTTCCAAGCTAATAATATCATTCTTCTATCCAAAGGTTTATTTTGAATTGTTGTAAT 12044 Query 205677 AATATGTGTTAATTGATCAAATCCTTGATTTGTATAATTTGTATCCATATCAATATATTT 205736 ||||| | ||| |||||| | || || | ||| |||| ||||||| | Sbjct 12043 AATATTTTTTAGTTGATCTATACCACCATATTTAT------TATCGCACCCAATATAATC 11990 Query 205737 AGCACCAAAATGACGCCATTGAAAACCATAAACTGGACCTAAATCACCTTCTTTTCTATC 205796 ||||| |||| | ||| ||||||||||||| || ||||||||| || ||| Sbjct 11989 TTCACCATAATGTCTCCACTGAAAACCATAAATAGGTCCTAAATCA--TTTTTT------ 11938 Query 205797 ATTAAAACCACGtttttttatatatttta--atgatccattttcatcccaaatatggatt 205854 |||||| | |||| || | ||| | || | |||||| || |||||| | | | Sbjct 11937 -TTAAAA--AGTTTTTGTTGTGTATAAAACGATTCTGCATTTTTATTCCAAATTTTAACT 11881 Query 205855 cctttattttttaGAATATTTGTATTAGTTGATCCAGAT-ATAAACCATAATAATTCTTC 205913 ||||| | |||| | | || | | ||| | | ||||||||||| | |||||| Sbjct 11880 CCTTTTTGATTTAATACAGAAACATCTG-TACTCCCGCTAATAAACCATAAGAGTTCTTC 11822 Query 205914 AACAATTCCTCTCCAAAACATTGATTTTGTTGTAAATAATGGAAATGAATCATCT--AAT 205971 |||| |||| ||||||| | | |||||| || | ||| ||||| | || ||| ||| Sbjct 11821 TACAACTCCTTTCCAAAAAACTTTTTTTGTAGTTAGTAACGGAAAAG-ATGTTCTTAAAT 11763 Query 205972 TTATATTTCATAGTTAAACCATATACAGTTTTTGTATCTACACCTGTTCTAT 206023 | | ||| || ||| |||| ||||| |||| || | | |||||||||| Sbjct 11762 TAAATTTTAATTGTT-CACCAAATACATTTTTAGTTCCAATTCCTGTTCTAT 11712 >seq297 Length=1033366 Score = 138 bits (152), Expect = 2e-27 Identities = 169/231 (73%), Gaps = 0/231 (0%) Strand=Plus/Plus Query 124538 AATGCAAATATTTATAAAGACACTAACAGGCAAAACTATAACTATAGAGATTGAAGCAAA 124597 |||||| || ||| | ||||| || |||||||| || || ||| | ||| |||| || Sbjct 233146 AATGCAGATCTTTGTCAAGACCCTCACAGGCAAGACCATTACTCTTGAGGTTGAGCCATC 233205 Query 124598 TGATACAATTTCTAATTTAAAACAGAAAATTCAAGATAAAGAAGGTATACCACCTGATCA 124657 ||||| ||| ||| | ||| ||||| ||||| ||||||||||| || || ||||| Sbjct 233206 AGATACCATTGAAAATGTCAAAGCTAAAATCCAAGACAAAGAAGGTATTCCCCCAGATCA 233265 Query 124658 ACAAAGGCTTATTTTTGCAGGAAAACAATTAGAGGATAGTCGAACATTAGAAGATTATAA 124717 || || | || ||||| ||||||||| |||| ||| |||| ||| | || || || Sbjct 233266 GCAGAGATTAATCTTTGCCGGAAAACAACTAGAAGATGGTCGCACACTTTCTGACTACAA 233325 Query 124718 TATTCAAAAAGAATCAACTTTGCATTTGGTTTTACGTTTACGAGGTGGTAT 124768 |||||||| || || || | ||| |||| || ||| | || |||||||| Sbjct 233326 CATTCAAAAGGAGTCTACCCTCCATCTGGTCTTGCGTCTCCGTGGTGGTAT 233376 Score = 122 bits (134), Expect = 1e-22 Identities = 165/230 (72%), Gaps = 0/230 (0%) Strand=Plus/Plus Query 124539 ATGCAAATATTTATAAAGACACTAACAGGCAAAACTATAACTATAGAGATTGAAGCAAAT 124598 ||||| || || | ||||| || || |||||||| || || | || ||||| | Sbjct 233831 ATGCAGATCTTCGTTAAGACTCTTACCGGCAAAACAATTACCCTGGAAGTTGAACCCTCA 233890 Query 124599 GATACAATTTCTAATTTAAAACAGAAAATTCAAGATAAAGAAGGTATACCACCTGATCAA 124658 || || ||| || | || ||||| ||||| ||||||||||| || || ||||| Sbjct 233891 GACACCATTGAAAACGTCAAGGCTAAAATCCAAGACAAAGAAGGTATTCCTCCAGATCAG 233950 Query 124659 CAAAGGCTTATTTTTGCAGGAAAACAATTAGAGGATAGTCGAACATTAGAAGATTATAAT 124718 || ||| | || || |||||||||||| |||| ||| | || || || |||||||| Sbjct 233951 CAGAGGTTGATCTTCGCAGGAAAACAACTAGAAGATGGACGTACCCTATCTGATTATAAC 234010 Query 124719 ATTCAAAAAGAATCAACTTTGCATTTGGTTTTACGTTTACGAGGTGGTAT 124768 || ||||||||||| ||| ||||| | || | ||| | || |||||||| Sbjct 234011 ATCCAAAAAGAATCCACTCTGCATCTTGTACTCCGTCTTCGTGGTGGTAT 234060 Score = 118 bits (130), Expect = 2e-21 Identities = 164/230 (71%), Gaps = 0/230 (0%) Strand=Plus/Plus Query 124539 ATGCAAATATTTATAAAGACACTAACAGGCAAAACTATAACTATAGAGATTGAAGCAAAT 124598 ||||| || || | ||||| || || |||||||| || || | || ||||| | Sbjct 233375 ATGCAGATCTTCGTTAAGACTCTTACTGGCAAAACAATTACCCTGGAAGTTGAACCCTCA 233434 Query 124599 GATACAATTTCTAATTTAAAACAGAAAATTCAAGATAAAGAAGGTATACCACCTGATCAA 124658 ||||| ||| ||| | ||| ||||| ||||| ||||||||||| || || ||||| Sbjct 233435 GATACTATTGAAAATGTCAAAGCTAAAATCCAAGACAAAGAAGGTATTCCCCCAGATCAG 233494 Query 124659 CAAAGGCTTATTTTTGCAGGAAAACAATTAGAGGATAGTCGAACATTAGAAGATTATAAT 124718 || || | || ||||| ||||||||| |||| ||| |||| ||| | || || ||| Sbjct 233495 CAGAGATTAATCTTTGCCGGAAAACAACTAGAAGATGGTCGCACACTTTCTGACTACAAT 233554 Query 124719 ATTCAAAAAGAATCAACTTTGCATTTGGTTTTACGTTTACGAGGTGGTAT 124768 || || || ||||| ||| | ||| | || | ||| | || |||||||| Sbjct 233555 ATCCAGAAGGAATCCACTCTCCATCTTGTACTCCGTCTTCGTGGTGGTAT 233604 Score = 114 bits (126), Expect = 2e-20 Identities = 162/228 (71%), Gaps = 0/228 (0%) Strand=Plus/Plus Query 124538 AATGCAAATATTTATAAAGACACTAACAGGCAAAACTATAACTATAGAGATTGAAGCAAA 124597 |||||| || ||| ||||||| || || |||||||| || || | || ||||| | Sbjct 234514 AATGCAGATCTTTGTAAAGACCCTTACCGGCAAAACCATTACCCTGGAAGTTGAACCCTC 234573 Query 124598 TGATACAATTTCTAATTTAAAACAGAAAATTCAAGATAAAGAAGGTATACCACCTGATCA 124657 ||||| ||| || | || ||||| ||||| || || ||||| || || ||||| Sbjct 234574 AGATACCATTGAAAACGTCAAGGCTAAAATCCAAGACAAGGAGGGTATTCCCCCAGATCA 234633 Query 124658 ACAAAGGCTTATTTTTGCAGGAAAACAATTAGAGGATAGTCGAACATTAGAAGATTATAA 124717 || ||| | || || ||||| |||||| |||| ||| | || || || |||||||| Sbjct 234634 GCAGAGGTTGATCTTCGCAGGCAAACAACTAGAAGATGGACGTACCCTATCTGATTATAA 234693 Query 124718 TATTCAAAAAGAATCAACTTTGCATTTGGTTTTACGTTTACGAGGTGG 124765 || ||||||||||| ||| ||||| | || | ||| | || || || Sbjct 234694 CATCCAAAAAGAATCCACTCTGCATCTTGTACTCCGTCTTCGTGGAGG 234741 Score = 109 bits (120), Expect = 8e-19 Identities = 162/230 (70%), Gaps = 0/230 (0%) Strand=Plus/Plus Query 124539 ATGCAAATATTTATAAAGACACTAACAGGCAAAACTATAACTATAGAGATTGAAGCAAAT 124598 ||||| || || |||| ||| | || || || ||||| || | ||| |||| || | Sbjct 234287 ATGCAGATCTTCGTAAAAACATTGACTGGAAAGACTATTACACTCGAGGTTGAGCCATCT 234346 Query 124599 GATACAATTTCTAATTTAAAACAGAAAATTCAAGATAAAGAAGGTATACCACCTGATCAA 124658 ||||| ||| ||| | ||| ||||| ||||| ||||||||||| || || ||||| Sbjct 234347 GATACCATTGAAAATGTCAAAGCTAAAATCCAAGACAAAGAAGGTATTCCCCCAGATCAG 234406 Query 124659 CAAAGGCTTATTTTTGCAGGAAAACAATTAGAGGATAGTCGAACATTAGAAGATTATAAT 124718 || ||| | || ||||| || |||||| |||| || |||| || | || || || Sbjct 234407 CAGAGGTTAATCTTTGCTGGCAAACAACTAGAAGACGGTCGCACGCTTTCTGACTACAAC 234466 Query 124719 ATTCAAAAAGAATCAACTTTGCATTTGGTTTTACGTTTACGAGGTGGTAT 124768 || || || ||||| ||| | ||| |||| || || | || ||||| || Sbjct 234467 ATCCAGAAGGAATCCACTCTCCATCTGGTCTTGCGACTCCGTGGTGGAAT 234516 Score = 104 bits (114), Expect = 4e-17 Identities = 161/230 (70%), Gaps = 0/230 (0%) Strand=Plus/Plus Query 124539 ATGCAAATATTTATAAAGACACTAACAGGCAAAACTATAACTATAGAGATTGAAGCAAAT 124598 ||||| || || | ||||| || || || |||||||| || | || ||||| | Sbjct 233603 ATGCAGATCTTCGTTAAGACCCTCACTGGTAAAACTATTACCTTGGAAGTTGAACCTTCA 233662 Query 124599 GATACAATTTCTAATTTAAAACAGAAAATTCAAGATAAAGAAGGTATACCACCTGATCAA 124658 ||||| ||| || | || ||||| ||||| ||||||||||| || |||||||| Sbjct 233663 GATACCATTGAAAACGTTAAGGCTAAAATCCAAGACAAAGAAGGTATTCCCCCTGATCAG 233722 Query 124659 CAAAGGCTTATTTTTGCAGGAAAACAATTAGAGGATAGTCGAACATTAGAAGATTATAAT 124718 || ||| | || || || || ||||| |||| ||| | |||| | || || || Sbjct 233723 CAGAGGTTGATCTTCGCTGGTAAACAGCTAGAAGATGGAAGAACTCTTTCGGACTACAAC 233782 Query 124719 ATTCAAAAAGAATCAACTTTGCATTTGGTTTTACGTTTACGAGGTGGTAT 124768 || || || ||||||||| | ||| |||| || ||| | || |||||||| Sbjct 233783 ATCCAGAAGGAATCAACTCTCCATCTGGTCTTGCGTCTCCGTGGTGGTAT 233832 Score = 95.1 bits (104), Expect = 2e-14 Identities = 159/230 (69%), Gaps = 0/230 (0%) Strand=Plus/Plus Query 124539 ATGCAAATATTTATAAAGACACTAACAGGCAAAACTATAACTATAGAGATTGAAGCAAAT 124598 ||||| || || |||| || || |||||||| || || ||| | ||| | || | Sbjct 232691 ATGCAGATCTTCGTAAAAACCCTCACAGGCAAGACCATCACTCTTGAGGTCGAGCCCTCA 232750 Query 124599 GATACAATTTCTAATTTAAAACAGAAAATTCAAGATAAAGAAGGTATACCACCTGATCAA 124658 || || ||| ||| | ||| || || ||||| || |||||||| || || ||||| Sbjct 232751 GACACCATTGAAAATGTGAAAGCCAAGATCCAAGACAAGGAAGGTATTCCCCCAGATCAG 232810 Query 124659 CAAAGGCTTATTTTTGCAGGAAAACAATTAGAGGATAGTCGAACATTAGAAGATTATAAT 124718 || ||| | || ||||||||||| ||| | || ||| |||| ||| | || || || Sbjct 232811 CAGAGGTTGATCTTTGCAGGAAAGCAACTGGAAGATGGTCGCACACTTTCTGACTACAAC 232870 Query 124719 ATTCAAAAAGAATCAACTTTGCATTTGGTTTTACGTTTACGAGGTGGTAT 124768 || || || || ||||| | ||| | || || || | || |||||||| Sbjct 232871 ATCCAGAAGGAGTCAACCCTCCATCTTGTCTTGCGACTCCGTGGTGGTAT 232920 Score = 84.2 bits (92), Expect = 3e-11 Identities = 176/260 (68%), Gaps = 3/260 (1%) Strand=Plus/Plus Query 124539 ATGCAAATATTTATAAAGACACTAACAGGCAAAACTATAACTATAGAGATTGAAGCAAAT 124598 ||||| || || | ||||| || || |||||||| || || | || ||||| | Sbjct 234059 ATGCAGATCTTCGTTAAGACCCTTACTGGCAAAACAATTACCCTGGAAGTTGAACCCTCA 234118 Query 124599 GATACAATTTCTAATTTAAAACAGAAAATTCAAGATAAAGAAGGTATACCACCTGATCAA 124658 || || ||| || | || ||||||||||| || ||||| || || || ||||| Sbjct 234119 GACACCATTGAAAACGTCAAGGCTAAAATTCAAGACAAGGAAGGCATTCCCCCAGATCAG 234178 Query 124659 CAAAGGCTTATTTTTGCAGGAAAACAATTAGAGGATAGTCGAACATTAGAAGATTATAAT 124718 || ||| | || |||||||| || || | || ||| | | ||| | || || ||| Sbjct 234179 CAGAGGTTGATCTTTGCAGGTAAGCAGCTGGAAGATGGAAGGACACTGTCTGACTACAAT 234238 Query 124719 ATTCAAAAAGAATCAACTTTGCATTTGGTTTTACGTTTACGAGGTGGTAT-TAAAAATTC 124777 || || || ||||| ||| | ||| | || | ||| | || |||||||| | | ||| Sbjct 234239 ATCCAGAAGGAATCCACTCTCCATCTTGTACTCCGTCTTCGTGGTGGTATGCAGATCTTC 234298 Query 124778 ATAAAA--ATTGATTGTAAA 124795 ||||| ||||| || ||| Sbjct 234299 GTAAAAACATTGACTGGAAA 234318 >seq1037 Length=250510 Score = 107 bits (118), Expect = 3e-18 Identities = 142/197 (72%), Gaps = 0/197 (0%) Strand=Plus/Plus Query 124539 ATGCAAATATTTATAAAGACACTAACAGGCAAAACTATAACTATAGAGATTGAAGCAAAT 124598 |||||||| ||| | || || || || ||||| ||||| || | || | || | | Sbjct 80654 ATGCAAATCTTTGTGAAAACCCTTACTGGCAAGACTATCACCCTTGAAGTAGAGCCCTCT 80713 Query 124599 GATACAATTTCTAATTTAAAACAGAAAATTCAAGATAAAGAAGGTATACCACCTGATCAA 124658 ||| ||||| ||| |||| || || || ||||| |||||||| || || ||||| Sbjct 80714 GATTCAATTGAGAATGTAAAGGCTAAGATCCAGGATAAGGAAGGTATCCCTCCAGATCAG 80773 Query 124659 CAAAGGCTTATTTTTGCAGGAAAACAATTAGAGGATAGTCGAACATTAGAAGATTATAAT 124718 |||||| | || ||||| || ||||||||||| || | || || || ||| || ||| Sbjct 80774 CAAAGGTTGATCTTTGCCGGCAAACAATTAGAAGACGGCCGCACTTTGTCAGACTACAAT 80833 Query 124719 ATTCAAAAAGAATCAAC 124735 || ||||||||||| || Sbjct 80834 ATCCAAAAAGAATCTAC 80850 Score = 107 bits (118), Expect = 3e-18 Identities = 142/197 (72%), Gaps = 0/197 (0%) Strand=Plus/Plus Query 124539 ATGCAAATATTTATAAAGACACTAACAGGCAAAACTATAACTATAGAGATTGAAGCAAAT 124598 |||||||| ||| ||||||| || || ||||| ||||| || | || | || | | Sbjct 84033 ATGCAAATCTTTGTAAAGACCCTTACTGGCAAGACTATCACCCTTGAAGTAGAGCCCTCT 84092 Query 124599 GATACAATTTCTAATTTAAAACAGAAAATTCAAGATAAAGAAGGTATACCACCTGATCAA 124658 || ||||| ||| |||| || || || ||||| |||||||| || || ||||| Sbjct 84093 GACTCAATTGAGAATGTAAAGGCTAAGATCCAGGATAAGGAAGGTATCCCCCCAGATCAG 84152 Query 124659 CAAAGGCTTATTTTTGCAGGAAAACAATTAGAGGATAGTCGAACATTAGAAGATTATAAT 124718 || ||| | || ||||| || ||||| ||||| ||| | || || || ||| || ||| Sbjct 84153 CAGAGGTTGATCTTTGCCGGTAAACAGTTAGAAGATGGCCGCACTTTGTCAGACTACAAT 84212 Query 124719 ATTCAAAAAGAATCAAC 124735 || ||||||||||| || Sbjct 84213 ATCCAAAAAGAATCTAC 84229 Score = 95.1 bits (104), Expect = 2e-14 Identities = 139/197 (71%), Gaps = 0/197 (0%) Strand=Plus/Plus Query 124539 ATGCAAATATTTATAAAGACACTAACAGGCAAAACTATAACTATAGAGATTGAAGCAAAT 124598 ||||| || || | || || || || ||||| ||||| || ||||| | || | Sbjct 81088 ATGCAGATCTTCGTGAAAACCCTTACGGGCAAGACTATTACCTTAGAGGTAGAGCCCTCA 81147 Query 124599 GATACAATTTCTAATTTAAAACAGAAAATTCAAGATAAAGAAGGTATACCACCTGATCAA 124658 || || || ||| |||| || || || ||||| |||||||| || || ||||| Sbjct 81148 GACACCATCGAAAATGTAAAGGCCAAGATCCAGGATAAGGAAGGTATCCCTCCAGATCAG 81207 Query 124659 CAAAGGCTTATTTTTGCAGGAAAACAATTAGAGGATAGTCGAACATTAGAAGATTATAAT 124718 || ||| | || ||||| || ||||||||||| ||| | || || || ||| || || Sbjct 81208 CAGAGGTTGATCTTTGCCGGTAAACAATTAGAAGATGGCCGCACTTTGTCAGACTACAAC 81267 Query 124719 ATTCAAAAAGAATCAAC 124735 || |||||||||||||| Sbjct 81268 ATCCAAAAAGAATCAAC 81284 Score = 95.1 bits (104), Expect = 2e-14 Identities = 139/197 (71%), Gaps = 0/197 (0%) Strand=Plus/Plus Query 124539 ATGCAAATATTTATAAAGACACTAACAGGCAAAACTATAACTATAGAGATTGAAGCAAAT 124598 |||||||| ||| | || || || || ||||| || || || | || | |||||| Sbjct 84261 ATGCAAATCTTTGTTAAAACTCTGACCGGCAAGACCATCACCCTTGAAGTAGAAGCATCA 84320 Query 124599 GATACAATTTCTAATTTAAAACAGAAAATTCAAGATAAAGAAGGTATACCACCTGATCAA 124658 || ||||| ||| | || || || || ||||| |||||||| || || ||||| Sbjct 84321 GACACAATCGAGAATGTTAAGGCTAAGATCCAGGATAAGGAAGGTATCCCTCCAGATCAG 84380 Query 124659 CAAAGGCTTATTTTTGCAGGAAAACAATTAGAGGATAGTCGAACATTAGAAGATTATAAT 124718 || ||| | || ||||| || |||||||| || || | || || ||| ||| || || Sbjct 84381 CAGAGGTTGATCTTTGCTGGTAAACAATTGGAAGACGGCCGTACCTTATCAGACTACAAC 84440 Query 124719 ATTCAAAAAGAATCAAC 124735 || ||||||||||| || Sbjct 84441 ATCCAAAAAGAATCTAC 84457 >seq472 Length=692712 Score = 86.0 bits (94), Expect = 9e-12 Identities = 132/193 (68%), Gaps = 0/193 (0%) Strand=Plus/Plus Query 202868 CCAACATATCCATTTGGATCACGTCCATCTATAAAATATTTATCATTTAAATNNNNNNNN 202927 |||||| | ||||| || ||||||||||| ||| | || ||||||| | | Sbjct 509667 CCAACAAAGCCATTAGGGTCACGTCCATCCATACTAAATCTATCATTGAGGTAGATAGCA 509726 Query 202928 NNNNNNAATGCTTCTTCTGGTGATTTTGTCCATTCTAATAtttttttAGCCCAATACATT 202987 || || ||||| ||||| | ||||| |||||||| || |||||||| ||||| Sbjct 509727 AAGGATAAGGCCTCTTCAGGTGAAGTGGTCCACTCTAATATCTTCTTAGCCCAGTACATA 509786 Query 202988 CTTAAATATCCATGCATTTTACCATAGATAATCATTTGTTTTTGTGATGCATTCCATAAA 203047 | ||| | ||||||||||| || | | | || ||| | || | || ||||| | | Sbjct 509787 CGCAAAAAACCATGCATTTTTCCCTCCTTGACCAGCTGTATCTGGGCTGAGTTCCAGAGA 509846 Query 203048 TTGTCATGAGTTA 203060 | ||||| |||| Sbjct 509847 TCATCATGGGTTA 509859 Lambda K H 0.634 0.408 0.912 Gapped Lambda K H 0.625 0.410 0.780 Effective search space used: 755264690211200