Alignment viewer
BLASTN 2.6.0+
Reference: Stephen F. Altschul, Thomas L. Madden, Alejandro A.
Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J.
Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of
protein database search programs", Nucleic Acids Res. 25:3389-3402.
Database: Megachile_20rotundata_20[refseq_20GCF_5F000220905.1_202011-07-19]
6,266 sequences; 272,660,569 total letters
Query= NC_002331.1
Length=178733
Score E
Sequences producing significant alignments: (Bits) Value
seq129 109 6e-19
seq2434 107 2e-18
seq64 107 2e-18
seq519 104 3e-17
seq307 104 3e-17
seq61 104 3e-17
seq133 95.1 1e-14
seq467 93.3 5e-14
seq453 87.8 2e-12
>seq129
Length=5769472
Score = 109 bits (120), Expect = 6e-19
Identities = 74/83 (89%), Gaps = 0/83 (0%)
Strand=Plus/Minus
Query 60057 AAAAATTTTCTCCTCGGCAGGGAATCGAACCCTGGTCTCCCGCGTGACAGGCGGACATAC 60116
||||| | |||| |||| ||||||||||||| ||||||||||||||||||||| ||||
Sbjct 2290220 AAAAAAATCCTCCCCGGCGGGGAATCGAACCCCGGTCTCCCGCGTGACAGGCGGGGATAC 2290161
Query 60117 TCACCACTATACTACCGAGGATT 60139
| |||||||||||||||||||||
Sbjct 2290160 TAACCACTATACTACCGAGGATT 2290138
>seq2434
Length=9448
Score = 107 bits (118), Expect = 2e-18
Identities = 75/84 (89%), Gaps = 1/84 (1%)
Strand=Plus/Minus
Query 60057 AAAAATTTTC-TCCTCGGCAGGGAATCGAACCCTGGTCTCCCGCGTGACAGGCGGACATA 60115
|| |||| || ||| |||| ||||||||||||| ||||||||||||||||||||| |||
Sbjct 5546 AATAATTATCCTCCCCGGCGGGGAATCGAACCCCGGTCTCCCGCGTGACAGGCGGGGATA 5487
Query 60116 CTCACCACTATACTACCGAGGATT 60139
|| |||||||||||||||||||||
Sbjct 5486 CTGACCACTATACTACCGAGGATT 5463
>seq64
Length=822164
Score = 107 bits (118), Expect = 2e-18
Identities = 68/74 (92%), Gaps = 0/74 (0%)
Strand=Plus/Minus
Query 60066 CTCCTCGGCAGGGAATCGAACCCTGGTCTCCCGCGTGACAGGCGGACATACTCACCACTA 60125
|||| |||| ||||||||||||| ||||||||||||||||||||| ||||| |||||||
Sbjct 388029 CTCCCCGGCGGGGAATCGAACCCCGGTCTCCCGCGTGACAGGCGGGGATACTGACCACTA 387970
Query 60126 TACTACCGAGGATT 60139
||||||||||||||
Sbjct 387969 TACTACCGAGGATT 387956
Score = 105 bits (116), Expect = 8e-18
Identities = 67/73 (92%), Gaps = 0/73 (0%)
Strand=Plus/Minus
Query 60065 TCTCCTCGGCAGGGAATCGAACCCTGGTCTCCCGCGTGACAGGCGGACATACTCACCACT 60124
||||| |||| ||||||||||||| ||||||||||||||||||||| ||||| ||||||
Sbjct 395339 TCTCCCCGGCGGGGAATCGAACCCCGGTCTCCCGCGTGACAGGCGGGGATACTGACCACT 395280
Query 60125 ATACTACCGAGGA 60137
|||||||||||||
Sbjct 395279 ATACTACCGAGGA 395267
>seq519
Length=14764
Score = 104 bits (114), Expect = 3e-17
Identities = 73/82 (89%), Gaps = 1/82 (1%)
Strand=Plus/Minus
Query 60057 AAAAATTTTC-TCCTCGGCAGGGAATCGAACCCTGGTCTCCCGCGTGACAGGCGGACATA 60115
|| |||| || ||| |||| ||||||||||||| ||||||||||||||||||||| |||
Sbjct 582 AATAATTATCCTCCCCGGCGGGGAATCGAACCCCGGTCTCCCGCGTGACAGGCGGGGATA 523
Query 60116 CTCACCACTATACTACCGAGGA 60137
|| |||||||||||||||||||
Sbjct 522 CTGACCACTATACTACCGAGGA 501
>seq307
Length=2169461
Score = 104 bits (114), Expect = 3e-17
Identities = 149/210 (71%), Gaps = 0/210 (0%)
Strand=Plus/Minus
Query 42975 ACCAAATGCAATGTGCTCTCCTTTTGAATATTGTAATCAGCCAGAGTCCGCTCGTCATCT 43034
|||| ||| | |||| || |||||||| |||||||||| || |||| | | || ||
Sbjct 1515880 ACCAGATGAAGTGTGGATTCTTTTTGAATGTTGTAATCAGAGAGTGTCCTCCCATCTTCG 1515821
Query 43035 AGCTGCTTACCAGCGAATATGAGTCGCTGCTGGTCGGCCGGCACGCTCTCCTTGTCCATG 43094
|||||||| || || || || | ||| ||||| || | || | | ||| |||||| |
Sbjct 1515820 AGCTGCTTTCCTGCAAAGATTAATCGTTGCTGATCTGGAGGTATTCCCTCTTTGTCCTGG 1515761
Query 43095 ATTTTTTGTTTGAGCGTCGCCACCGTGTCGCTCGATTCCACATCGACCGTGATGGTTTTG 43154
|| || ||||| || | | ||||| | ||| || || | |||||||||||
Sbjct 1515760 ATCTTAGCTTTGACATTCTCTATTGTGTCAGAAGCTTCGACTTCCAGGGTGATGGTTTTT 1515701
Query 43155 CCCGTTAGCGTCTTTACAAAGATTTGCATT 43184
|| || || ||||| || ||||||||||||
Sbjct 1515700 CCTGTGAGAGTCTTGACGAAGATTTGCATT 1515671
Score = 93.3 bits (102), Expect = 5e-14
Identities = 158/229 (69%), Gaps = 0/229 (0%)
Strand=Plus/Minus
Query 42956 TCCTCCTCTTAAACGCAACACCAAATGCAATGTGCTCTCCTTTTGAATATTGTAATCAGC 43015
|||||||| | | ||| |||| ||| | |||| || |||||||| ||||||||||
Sbjct 1515443 TCCTCCTCGAAGTCTCAAAACCAGATGAAGTGTGGATTCTTTTTGAATGTTGTAATCAGA 1515384
Query 43016 CAGAGTCCGCTCGTCATCTAGCTGCTTACCAGCGAATATGAGTCGCTGCTGGTCGGCCGG 43075
|| |||| | || || || || || ||||| || || | ||| ||||| | | ||
Sbjct 1515383 GAGTGTCCTTCCATCTTCAAGTTGTTTCCCAGCAAAGATTAATCGTTGCTGATGAGGGGG 1515324
Query 43076 CACGCTCTCCTTGTCCATGATTTTTTGTTTGAGCGTCGCCACCGTGTCGCTCGATTCCAC 43135
| | ||| |||||| ||| ||| || | | | | ||||| | ||| ||
Sbjct 1515323 AATTCCCTCTTTGTCCTGGATCTTTGCCTTTACATTTTCAATTGTGTCAGAAGCTTCGAC 1515264
Query 43136 ATCGACCGTGATGGTTTTGCCCGTTAGCGTCTTTACAAAGATTTGCATT 43184
|| | ||||||||||| || || || ||||| |||||||||||||||
Sbjct 1515263 TTCCAGAGTGATGGTTTTCCCTGTGAGTGTCTTGACAAAGATTTGCATT 1515215
Score = 91.5 bits (100), Expect = 2e-13
Identities = 146/210 (70%), Gaps = 0/210 (0%)
Strand=Plus/Minus
Query 42975 ACCAAATGCAATGTGCTCTCCTTTTGAATATTGTAATCAGCCAGAGTCCGCTCGTCATCT 43034
|||| ||| | |||| || |||||||| || ||||| | ||| |||| | || ||
Sbjct 1510755 ACCAGATGAAGTGTGGATTCTTTTTGAATGTTATAATCTGACAGTGTCCTTCCATCTTCG 1510696
Query 43035 AGCTGCTTACCAGCGAATATGAGTCGCTGCTGGTCGGCCGGCACGCTCTCCTTGTCCATG 43094
|||||||| || || || || | ||| |||||||| | || | | || ||||| |
Sbjct 1510695 AGCTGCTTCCCTGCAAAGATTAATCGTTGCTGGTCTGGAGGAATTCCTTCTTTGTCTTGG 1510636
Query 43095 ATTTTTTGTTTGAGCGTCGCCACCGTGTCGCTCGATTCCACATCGACCGTGATGGTTTTG 43154
|| || ||||| || | | ||||| | |||||| || | ||||| |||||
Sbjct 1510635 ATCTTAGCTTTGACATTCTCTATTGTGTCTGAGGCTTCCACTTCTAGGGTGATAGTTTTC 1510576
Query 43155 CCCGTTAGCGTCTTTACAAAGATTTGCATT 43184
|| || || ||||| || ||||||||||||
Sbjct 1510575 CCTGTGAGAGTCTTCACGAAGATTTGCATT 1510546
Score = 91.5 bits (100), Expect = 2e-13
Identities = 146/210 (70%), Gaps = 0/210 (0%)
Strand=Plus/Minus
Query 42975 ACCAAATGCAATGTGCTCTCCTTTTGAATATTGTAATCAGCCAGAGTCCGCTCGTCATCT 43034
|||| ||| | ||| || |||||||| || ||||| | ||| |||| | | || ||
Sbjct 1515652 ACCAGATGAAGGGTGGATTCTTTTTGAATGTTATAATCTGACAGTGTCCTCCCATCTTCG 1515593
Query 43035 AGCTGCTTACCAGCGAATATGAGTCGCTGCTGGTCGGCCGGCACGCTCTCCTTGTCCATG 43094
|||||||| || || || || | ||| |||||||| | || | | || ||||| |
Sbjct 1515592 AGCTGCTTTCCTGCAAAGATTAATCGTTGCTGGTCTGGAGGAATTCCTTCTTTGTCTTGG 1515533
Query 43095 ATTTTTTGTTTGAGCGTCGCCACCGTGTCGCTCGATTCCACATCGACCGTGATGGTTTTG 43154
|| || ||||| || | | ||||| | |||||| || | ||||| |||||
Sbjct 1515532 ATCTTAGCTTTGACATTCTCTATTGTGTCTGAGGCTTCCACTTCTAGGGTGATAGTTTTC 1515473
Query 43155 CCCGTTAGCGTCTTTACAAAGATTTGCATT 43184
|| || || ||||| || ||||||||||||
Sbjct 1515472 CCTGTGAGAGTCTTCACGAAGATTTGCATT 1515443
Score = 86.0 bits (94), Expect = 7e-12
Identities = 145/210 (69%), Gaps = 0/210 (0%)
Strand=Plus/Minus
Query 42975 ACCAAATGCAATGTGCTCTCCTTTTGAATATTGTAATCAGCCAGAGTCCGCTCGTCATCT 43034
|||| ||| | |||| || |||||||| || ||||| | ||| |||| | || ||
Sbjct 1510071 ACCAGATGAAGTGTGGATTCTTTTTGAATGTTATAATCTGACAGTGTCCTTCCATCTTCG 1510012
Query 43035 AGCTGCTTACCAGCGAATATGAGTCGCTGCTGGTCGGCCGGCACGCTCTCCTTGTCCATG 43094
|||||||| || || || || | ||| |||||||| | || | | ||| |||||| |
Sbjct 1510011 AGCTGCTTCCCTGCAAAGATTAATCGTTGCTGGTCTGGAGGGATTCCCTCTTTGTCCTGG 1509952
Query 43095 ATTTTTTGTTTGAGCGTCGCCACCGTGTCGCTCGATTCCACATCGACCGTGATGGTTTTG 43154
|| || || | | | | ||||| | ||| || || | |||||||||||
Sbjct 1509951 ATCTTCGCCTTTACATTTTCAATTGTGTCAGAAGCTTCGACTTCCAGAGTGATGGTTTTC 1509892
Query 43155 CCCGTTAGCGTCTTTACAAAGATTTGCATT 43184
|| || || ||||| || ||||||||||||
Sbjct 1509891 CCTGTGAGTGTCTTCACGAAGATTTGCATT 1509862
Score = 86.0 bits (94), Expect = 7e-12
Identities = 145/210 (69%), Gaps = 0/210 (0%)
Strand=Plus/Minus
Query 42975 ACCAAATGCAATGTGCTCTCCTTTTGAATATTGTAATCAGCCAGAGTCCGCTCGTCATCT 43034
|||| ||| | |||| || |||||||| || ||||| | ||| |||| | || ||
Sbjct 1510299 ACCAGATGAAGTGTGGATTCTTTTTGAATGTTATAATCTGACAGTGTCCTTCCATCTTCG 1510240
Query 43035 AGCTGCTTACCAGCGAATATGAGTCGCTGCTGGTCGGCCGGCACGCTCTCCTTGTCCATG 43094
|||||||| || || || || | ||| |||||||| | || | | ||| |||||| |
Sbjct 1510239 AGCTGCTTCCCTGCAAAGATTAATCGTTGCTGGTCTGGAGGGATTCCCTCTTTGTCCTGG 1510180
Query 43095 ATTTTTTGTTTGAGCGTCGCCACCGTGTCGCTCGATTCCACATCGACCGTGATGGTTTTG 43154
|| || || | | | | ||||| | ||| || || | |||||||||||
Sbjct 1510179 ATCTTCGCCTTTACATTTTCAATTGTGTCAGAAGCTTCGACTTCCAGGGTGATGGTTTTT 1510120
Query 43155 CCCGTTAGCGTCTTTACAAAGATTTGCATT 43184
|| || || ||||| || ||||||||||||
Sbjct 1510119 CCTGTGAGAGTCTTGACGAAGATTTGCATT 1510090
Score = 86.0 bits (94), Expect = 7e-12
Identities = 145/210 (69%), Gaps = 0/210 (0%)
Strand=Plus/Minus
Query 42975 ACCAAATGCAATGTGCTCTCCTTTTGAATATTGTAATCAGCCAGAGTCCGCTCGTCATCT 43034
|||| ||| | |||| || |||||||| || ||||| | ||| |||| | || ||
Sbjct 1510527 ACCAGATGAAGTGTGGATTCTTTTTGAATGTTATAATCTGACAGTGTCCTTCCATCTTCG 1510468
Query 43035 AGCTGCTTACCAGCGAATATGAGTCGCTGCTGGTCGGCCGGCACGCTCTCCTTGTCCATG 43094
|||||||| || || || || | ||| |||||||| | || | | ||| |||||| |
Sbjct 1510467 AGCTGCTTCCCTGCAAAGATTAATCGTTGCTGGTCTGGAGGGATTCCCTCTTTGTCCTGG 1510408
Query 43095 ATTTTTTGTTTGAGCGTCGCCACCGTGTCGCTCGATTCCACATCGACCGTGATGGTTTTG 43154
|| || || | | | | ||||| | ||| || || | |||||||||||
Sbjct 1510407 ATCTTCGCCTTTACATTTTCAATTGTGTCAGAAGCTTCGACTTCCAGGGTGATGGTTTTT 1510348
Query 43155 CCCGTTAGCGTCTTTACAAAGATTTGCATT 43184
|| || || ||||| || ||||||||||||
Sbjct 1510347 CCTGTGAGAGTCTTGACGAAGATTTGCATT 1510318
Score = 86.0 bits (94), Expect = 7e-12
Identities = 134/192 (70%), Gaps = 0/192 (0%)
Strand=Plus/Minus
Query 42993 TCCTTTTGAATATTGTAATCAGCCAGAGTCCGCTCGTCATCTAGCTGCTTACCAGCGAAT 43052
|| ||||||||||| ||||| | || |||| | || || |||||||| ||||| ||
Sbjct 1514715 TCTTTTTGAATATTATAATCTGAAAGTGTCCTTCCATCTTCCAGCTGCTTTCCAGCAAAG 1514656
Query 43053 ATGAGTCGCTGCTGGTCGGCCGGCACGCTCTCCTTGTCCATGATTTTTTGTTTGAGCGTC 43112
|| | ||| ||||| || | || | | || || || ||| || ||||| ||
Sbjct 1514655 ATTAATCGTTGCTGATCAGGAGGAATTCCTTCTTTATCTTGGATCTTAGCTTTGACATTC 1514596
Query 43113 GCCACCGTGTCGCTCGATTCCACATCGACCGTGATGGTTTTGCCCGTTAGCGTCTTTACA 43172
| | ||| || | ||| || || | ||||||||||| || || || ||||| |||
Sbjct 1514595 TCTATCGTATCTGAAGCTTCGACTTCCAGGGTGATGGTTTTCCCTGTGAGTGTCTTGACA 1514536
Query 43173 AAGATTTGCATT 43184
||||||||||||
Sbjct 1514535 AAGATTTGCATT 1514524
Score = 86.0 bits (94), Expect = 7e-12
Identities = 145/210 (69%), Gaps = 0/210 (0%)
Strand=Plus/Minus
Query 42975 ACCAAATGCAATGTGCTCTCCTTTTGAATATTGTAATCAGCCAGAGTCCGCTCGTCATCT 43034
|||| ||| | |||| || |||||||| || ||||| | ||| |||| | || ||
Sbjct 1514961 ACCAGATGAAGTGTGGATTCTTTTTGAATGTTATAATCTGACAGTGTCCTTCCATCTTCG 1514902
Query 43035 AGCTGCTTACCAGCGAATATGAGTCGCTGCTGGTCGGCCGGCACGCTCTCCTTGTCCATG 43094
|||||||| || || || || | ||| |||||||| | || | | ||| |||||| |
Sbjct 1514901 AGCTGCTTCCCTGCAAAGATTAATCGTTGCTGGTCTGGAGGGATTCCCTCTTTGTCCTGG 1514842
Query 43095 ATTTTTTGTTTGAGCGTCGCCACCGTGTCGCTCGATTCCACATCGACCGTGATGGTTTTG 43154
|| || || | | | | ||||| | ||| || || | |||||||||||
Sbjct 1514841 ATCTTCGCCTTTACATTTTCAATTGTGTCAGAAGCTTCGACTTCCAGGGTGATGGTTTTT 1514782
Query 43155 CCCGTTAGCGTCTTTACAAAGATTTGCATT 43184
|| || || ||||| || ||||||||||||
Sbjct 1514781 CCTGTGAGAGTCTTGACGAAGATTTGCATT 1514752
>seq61
Length=1771752
Score = 104 bits (114), Expect = 3e-17
Identities = 68/75 (91%), Gaps = 0/75 (0%)
Strand=Plus/Minus
Query 60066 CTCCTCGGCAGGGAATCGAACCCTGGTCTCCCGCGTGACAGGCGGACATACTCACCACTA 60125
|||| |||| ||||||||||||| ||||||||||||||||||||| ||||| |||||||
Sbjct 510156 CTCCCCGGCGGGGAATCGAACCCCGGTCTCCCGCGTGACAGGCGGGGATACTAACCACTA 510097
Query 60126 TACTACCGAGGATTG 60140
|||||||||||| ||
Sbjct 510096 TACTACCGAGGAATG 510082
Score = 95.1 bits (104), Expect = 1e-14
Identities = 64/72 (89%), Gaps = 0/72 (0%)
Strand=Plus/Minus
Query 60066 CTCCTCGGCAGGGAATCGAACCCTGGTCTCCCGCGTGACAGGCGGACATACTCACCACTA 60125
|||| || | ||||||||||||| ||||||||||||||||||||| ||||| |||||||
Sbjct 510998 CTCCCCGACGGGGAATCGAACCCCGGTCTCCCGCGTGACAGGCGGGGATACTAACCACTA 510939
Query 60126 TACTACCGAGGA 60137
||||| ||||||
Sbjct 510938 TACTATCGAGGA 510927
>seq133
Length=1601264
Score = 95.1 bits (104), Expect = 1e-14
Identities = 64/72 (89%), Gaps = 0/72 (0%)
Strand=Plus/Minus
Query 60066 CTCCTCGGCAGGGAATCGAACCCTGGTCTCCCGCGTGACAGGCGGACATACTCACCACTA 60125
|||| || | ||||||||||||| ||||||||||||||||||||| ||||| |||||||
Sbjct 293275 CTCCCCGACGGGGAATCGAACCCCGGTCTCCCGCGTGACAGGCGGGGATACTAACCACTA 293216
Query 60126 TACTACCGAGGA 60137
||||| ||||||
Sbjct 293215 TACTATCGAGGA 293204
>seq467
Length=2555529
Score = 93.3 bits (102), Expect = 5e-14
Identities = 135/191 (71%), Gaps = 0/191 (0%)
Strand=Plus/Plus
Query 42993 TCCTTTTGAATATTGTAATCAGCCAGAGTCCGCTCGTCATCTAGCTGCTTACCAGCGAAT 43052
|||||||| |||||||| |||| || || || | || |||| || |||||||||||
Sbjct 1366380 TCCTTTTGTATATTGTAGTCAGAAAGTGTGCGTCCATCTTCTAATTGTTTACCAGCGAAG 1366439
Query 43053 ATGAGTCGCTGCTGGTCGGCCGGCACGCTCTCCTTGTCCATGATTTTTTGTTTGAGCGTC 43112
|| | || |||||| || | || | || ||| || ||| ||||||| ||| | ||
Sbjct 1366440 ATCAATCTCTGCTGATCTGGTGGGATGCCCTCTTTATCCTGGATTTTTGCTTTAACATTC 1366499
Query 43113 GCCACCGTGTCGCTCGATTCCACATCGACCGTGATGGTTTTGCCCGTTAGCGTCTTTACA 43172
| | ||||| | || || |||| || |||||||| ||||| | ||||||||
Sbjct 1366500 TCGATGGTGTCTGATGCCTCAACCTCGAGGGTTATGGTTTTTCCCGTCAATGTCTTTACG 1366559
Query 43173 AAGATTTGCAT 43183
|| || |||||
Sbjct 1366560 AAAATCTGCAT 1366570
Score = 86.0 bits (94), Expect = 7e-12
Identities = 159/231 (69%), Gaps = 2/231 (1%)
Strand=Plus/Plus
Query 42955 GTCCTCCTCTTAAACGCAACACCAAATGCAATGTGCTCTCCTTTTGAATATTGTAATCAG 43014
||||||| || | || | || ||||| | || || || ||||||||||||||||
Sbjct 1364157 GTCCTCCCCTGAGTCGAAGTACTAAATGGAGAGTAGATTCTTTCTGAATATTGTAATCAG 1364216
Query 43015 CCAGAGTCCGCTCGTCATCTAGCTGCTTACCAGCGAATATGAGTCGCTGCTGGTCGGCCG 43074
|| || || | || || || || ||||||||||| || | || |||||| || | |
Sbjct 1364217 AAAGTGTACGACCATCTTCCAGTTGTTTACCAGCGAAGATTAATCTCTGCTGATCTGGTG 1364276
Query 43075 GCACGCTCTCCTTGTCCATGATTTTTTG-TTTGAGCGTCGCCACCGTGTCGCTCGATTCC 43133
| | || || |||| | ||| |||||| || | | | | ||||| || ||
Sbjct 1364277 GAATGCCTTCTTTGT-CTTGAATTTTTGCCTTCACGTTTTCAATAGTGTCAGACGCCTCA 1364335
Query 43134 ACATCGACCGTGATGGTTTTGCCCGTTAGCGTCTTTACAAAGATTTGCATT 43184
|| || | || |||||||| || || || ||||| ||||| |||||||||
Sbjct 1364336 ACTTCCAGAGTTATGGTTTTTCCAGTAAGAGTCTTCACAAAAATTTGCATT 1364386
>seq453
Length=4956680
Score = 87.8 bits (96), Expect = 2e-12
Identities = 63/73 (86%), Gaps = 0/73 (0%)
Strand=Plus/Minus
Query 60066 CTCCTCGGCAGGGAATCGAACCCTGGTCTCCCGCGTGACAGGCGGACATACTCACCACTA 60125
|||| || | |||| |||||||| ||||||||||||||||||||| ||||| ||| |||
Sbjct 4254961 CTCCCCGACGGGGAGTCGAACCCCGGTCTCCCGCGTGACAGGCGGGGATACTAACCGCTA 4254902
Query 60126 TACTACCGAGGAT 60138
||||| |||||||
Sbjct 4254901 TACTATCGAGGAT 4254889
Lambda K H
0.634 0.408 0.912
Gapped
Lambda K H
0.625 0.410 0.780
Effective search space used: 571772812386480
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