Alignment viewer

BLASTN 2.6.0+ Reference: Stephen F. Altschul, Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Database: Cynoglossus_20semilaevis_20[refseq_20GCF_5F000523025.1_202014-01-28] 29,643 sequences; 470,199,494 total letters Query= NC_029692.1 Length=362276 Score E Sequences producing significant alignments: (Bits) Value seq19 179 9e-40 seq14 143 6e-29 seq15 98.7 2e-15 >seq19 Length=17744385 Score = 179 bits (198), Expect = 9e-40 Identities = 170/216 (79%), Gaps = 6/216 (3%) Strand=Plus/Minus Query 210903 AGGACAAGGTGAAGAGTGGACTCTTTTTGGATGTTATAATCCGCAAGGGTCCTTCCATCT 210962 |||||||| || || ||||||||||| |||||||| || || | |||| | |||||| Sbjct 3993456 AGGACAAGATGGAGGGTGGACTCTTTCTGGATGTTGTAGTCAGAGAGGGGGCGGCCATCT 3993397 Query 210963 TCCAGCTGCTTGCCTGCGAAGATAAGGCGTTGTTGGTCAGGAGGAACTCCTTCCTTGTCC 211022 |||||||| ||||| || ||||| || | ||| || || || |||| |||||||||||| Sbjct 3993396 TCCAGCTGTTTGCCAGCAAAGATCAGCCTTTGCTGATCGGGGGGAATGCCTTCCTTGTCC 3993337 Query 211023 TGGATCTTTTGTTTCACGTTTTCGATGGTGTCAGAGCTT---TCAACCTCGAGAGTGATC 211079 ||||||||| ||||| || || || |||||| ||| |||||||| || ||||| Sbjct 3993336 TGGATCTTTGCCTTCACATTCTCAATAGTGTCA---CTTGGCTCAACCTCAAGGGTGATG 3993280 Query 211080 GTCTTTCCCGTGAGAGTCTTTACAAACACCTGCATT 211115 ||||| || ||||| ||||||||||| | ||||||| Sbjct 3993279 GTCTTGCCAGTGAGGGTCTTTACAAAGATCTGCATT 3993244 Score = 161 bits (178), Expect = 2e-34 Identities = 169/222 (76%), Gaps = 0/222 (0%) Strand=Plus/Plus Query 210894 CGAAGTCTTAGGACAAGGTGAAGAGTGGACTCTTTTTGGATGTTATAATCCGCAAGGGTC 210953 ||||| | || ||||| || ||||||||||| || |||||||| || || | || || Sbjct 2912391 CGAAGGCGAAGCACAAGATGTAGAGTGGACTCCTTCTGGATGTTGTAGTCAGAGAGAGTG 2912450 Query 210954 CTTCCATCTTCCAGCTGCTTGCCTGCGAAGATAAGGCGTTGTTGGTCAGGAGGAACTCCT 211013 | ||||| ||||||||||| || || ||||| || || || ||||| || || | || Sbjct 2912451 CGACCATCCTCCAGCTGCTTTCCAGCAAAGATGAGACGCTGCTGGTCTGGTGGGATGCCC 2912510 Query 211014 TCCTTGTCCTGGATCTTTTGTTTCACGTTTTCGATGGTGTCAGAGCTTTCAACCTCGAGA 211073 || |||||||||||||| ||||||||| || |||||||| | |||||| || | Sbjct 2912511 TCTTTGTCCTGGATCTTGGCTTTCACGTTCTCAATGGTGTCGCTGGGTTCAACTTCCAAG 2912570 Query 211074 GTGATCGTCTTTCCCGTGAGAGTCTTTACAAACACCTGCATT 211115 ||||| ||||| || || || || || ||||||||||||||| Sbjct 2912571 GTGATGGTCTTGCCAGTCAGGGTTTTCACAAACACCTGCATT 2912612 Score = 161 bits (178), Expect = 2e-34 Identities = 161/209 (77%), Gaps = 0/209 (0%) Strand=Plus/Plus Query 210906 ACAAGGTGAAGAGTGGACTCTTTTTGGATGTTATAATCCGCAAGGGTCCTTCCATCTTCC 210965 ||||| || || |||||||| || ||||||||||| || | || || | ||||| ||| Sbjct 2914313 ACAAGATGGAGGGTGGACTCCTTCTGGATGTTATAGTCAGAGAGAGTGCGGCCATCCTCC 2914372 Query 210966 AGCTGCTTGCCTGCGAAGATAAGGCGTTGTTGGTCAGGAGGAACTCCTTCCTTGTCCTGG 211025 ||||| |||||||| || || || | || ||||| ||||||| || |||||||||||| Sbjct 2914373 AGCTGTTTGCCTGCAAATATGAGTCTCTGCTGGTCTGGAGGAATGCCCTCCTTGTCCTGG 2914432 Query 211026 ATCTTTTGTTTCACGTTTTCGATGGTGTCAGAGCTTTCAACCTCGAGAGTGATCGTCTTT 211085 ||||| ||||||||| || || ||||| | || ||||| || ||||| ||||| Sbjct 2914433 ATCTTGGATTTCACGTTCTCTATTGTGTCGCTGGGCTCGACCTCCAGGGTGATGGTCTTG 2914492 Query 211086 CCCGTGAGAGTCTTTACAAACACCTGCAT 211114 || || || ||||| ||||||| |||||| Sbjct 2914493 CCAGTTAGGGTCTTGACAAACATCTGCAT 2914521 Score = 161 bits (178), Expect = 2e-34 Identities = 163/212 (77%), Gaps = 0/212 (0%) Strand=Plus/Minus Query 210903 AGGACAAGGTGAAGAGTGGACTCTTTTTGGATGTTATAATCCGCAAGGGTCCTTCCATCT 210962 |||||||| || || ||||||||||| |||||||| || || | ||||| | |||||| Sbjct 3999189 AGGACAAGATGGAGGGTGGACTCTTTCTGGATGTTGTAGTCAGAGAGGGTGCGGCCATCT 3999130 Query 210963 TCCAGCTGCTTGCCTGCGAAGATAAGGCGTTGTTGGTCAGGAGGAACTCCTTCCTTGTCC 211022 |||||||||||||| || ||||| || | || || ||||| |||| || ||||||||| Sbjct 3999129 TCCAGCTGCTTGCCAGCAAAGATCAGCCTCTGCTGATCAGGGGGAATGCCCTCCTTGTCC 3999070 Query 211023 TGGATCTTTTGTTTCACGTTTTCGATGGTGTCAGAGCTTTCAACCTCGAGAGTGATCGTC 211082 ||||||||| || || || || ||||||||| | || || || || || || ||| Sbjct 3999069 TGGATCTTTGCCTTTACATTCTCAATGGTGTCACTGGGCTCTACTTCAAGGGTAATGGTC 3999010 Query 211083 TTTCCCGTGAGAGTCTTTACAAACACCTGCAT 211114 || || ||||| || || ||||| | |||||| Sbjct 3999009 TTGCCAGTGAGGGTTTTGACAAAGATCTGCAT 3998978 Score = 158 bits (174), Expect = 3e-33 Identities = 162/212 (76%), Gaps = 0/212 (0%) Strand=Plus/Minus Query 210903 AGGACAAGGTGAAGAGTGGACTCTTTTTGGATGTTATAATCCGCAAGGGTCCTTCCATCT 210962 |||||||| || || ||||| ||||| |||||||| || || | ||||| | |||||| Sbjct 3993684 AGGACAAGATGGAGGGTGGATTCTTTCTGGATGTTGTAGTCAGAGAGGGTGCGGCCATCT 3993625 Query 210963 TCCAGCTGCTTGCCTGCGAAGATAAGGCGTTGTTGGTCAGGAGGAACTCCTTCCTTGTCC 211022 |||||||| ||||| || ||||| || | || || ||||| |||| |||||||||||| Sbjct 3993624 TCCAGCTGTTTGCCAGCAAAGATCAGCCTCTGCTGATCAGGGGGAATGCCTTCCTTGTCC 3993565 Query 211023 TGGATCTTTTGTTTCACGTTTTCGATGGTGTCAGAGCTTTCAACCTCGAGAGTGATCGTC 211082 ||||| || ||||| || || ||||||||| | |||||||| || || || ||| Sbjct 3993564 TGGATTTTGGCCTTCACATTCTCAATGGTGTCACTGGGCTCAACCTCAAGGGTAATGGTC 3993505 Query 211083 TTTCCCGTGAGAGTCTTTACAAACACCTGCAT 211114 || || ||||| || || || || | |||||| Sbjct 3993504 TTGCCAGTGAGGGTTTTGACGAAGATCTGCAT 3993473 Score = 152 bits (168), Expect = 1e-31 Identities = 161/212 (76%), Gaps = 0/212 (0%) Strand=Plus/Minus Query 210903 AGGACAAGGTGAAGAGTGGACTCTTTTTGGATGTTATAATCCGCAAGGGTCCTTCCATCT 210962 |||||||| || || ||||||||||| |||||||| || || | ||||| | |||||| Sbjct 3999417 AGGACAAGATGGAGGGTGGACTCTTTCTGGATGTTGTAGTCAGAAAGGGAGCGGCCATCT 3999358 Query 210963 TCCAGCTGCTTGCCTGCGAAGATAAGGCGTTGTTGGTCAGGAGGAACTCCTTCCTTGTCC 211022 |||||||||||||| || ||||| || | || || ||||| |||| |||||||||||| Sbjct 3999357 TCCAGCTGCTTGCCAGCAAAGATCAGCCTCTGCTGATCAGGGGGAATGCCTTCCTTGTCC 3999298 Query 211023 TGGATCTTTTGTTTCACGTTTTCGATGGTGTCAGAGCTTTCAACCTCGAGAGTGATCGTC 211082 ||||| || || || || || ||||||||| | || || || || || || ||| Sbjct 3999297 TGGATTTTGGCCTTTACATTCTCAATGGTGTCACTGGGCTCTACTTCAAGGGTAATGGTC 3999238 Query 211083 TTTCCCGTGAGAGTCTTTACAAACACCTGCAT 211114 || || ||||| || || || || | |||||| Sbjct 3999237 TTGCCAGTGAGGGTTTTGACGAAGATCTGCAT 3999206 Score = 145 bits (160), Expect = 2e-29 Identities = 162/216 (75%), Gaps = 4/216 (2%) Strand=Plus/Minus Query 210903 AGGACAAGGTGAAGAGTGGACTCTTTTTGGATGTTATAATCCGCAAGGGTCCTTCCATCT 210962 |||||||| ||||| || || |||||||||||||| || || | || || | |||||| Sbjct 3998961 AGGACAAGATGAAGCGTAGATTCTTTTTGGATGTTGTAGTCGGACAGAGTACGACCATCT 3998902 Query 210963 TCCAGCTGCTTGCCTGCGAAGATAAGGCGTTGTTGGTCAGGAGGAACTCCTTCCTTGTCC 211022 ||||||||||| || || ||||| || | || || ||||| |||| |||||||||||| Sbjct 3998901 TCCAGCTGCTTTCCAGCAAAGATCAGCCTCTGCTGATCAGGGGGAATGCCTTCCTTGTCC 3998842 Query 211023 TGGATCTTTTGTTTCACGTTTTCGATGGTGTCAGAGCTTTCAACCTCGAGAGTGATCGTC 211082 ||||| || || || ||||| ||||||||| | || || || || || || ||| Sbjct 3998841 TGGATTTTAGCCTTTACATTTTCAATGGTGTCACTGGGCTCTACTTCAAGGGTAATGGTC 3998782 Query 211083 TTTCC----CGTGAGAGTCTTTACAAACACCTGCAT 211114 || || ||||| ||||| ||||| | |||||| Sbjct 3998781 TTGCCAGTGAGTGAGGGTCTTCACAAAAATCTGCAT 3998746 Score = 131 bits (144), Expect = 4e-25 Identities = 156/212 (74%), Gaps = 0/212 (0%) Strand=Plus/Plus Query 210903 AGGACAAGGTGAAGAGTGGACTCTTTTTGGATGTTATAATCCGCAAGGGTCCTTCCATCT 210962 || || || || ||||||||||| || |||||||| || || | || || | ||||| Sbjct 2910138 AGAACGAGATGTAGAGTGGACTCCTTCTGGATGTTGTAGTCGGAGAGAGTGCGACCATCC 2910197 Query 210963 TCCAGCTGCTTGCCTGCGAAGATAAGGCGTTGTTGGTCAGGAGGAACTCCTTCCTTGTCC 211022 || ||||| ||||| || ||||| || || || ||||| ||||||| ||||| || ||| Sbjct 2910198 TCTAGCTGTTTGCCAGCAAAGATGAGACGCTGCTGGTCTGGAGGAATGCCTTCTTTATCC 2910257 Query 211023 TGGATCTTTTGTTTCACGTTTTCGATGGTGTCAGAGCTTTCAACCTCGAGAGTGATCGTC 211082 ||||| || ||| | ||||| || |||||| || |||| |||||||| ||| Sbjct 2910258 TGGATTTTAGCTTTGATATTTTCAATTGTGTCACTAGGCTCCGCCTCCAGAGTGATAGTC 2910317 Query 211083 TTTCCCGTGAGAGTCTTTACAAACACCTGCAT 211114 || || || || || || ||||| | |||||| Sbjct 2910318 TTGCCAGTCAGGGTTTTGACAAAGATCTGCAT 2910349 Score = 131 bits (144), Expect = 4e-25 Identities = 158/215 (73%), Gaps = 0/215 (0%) Strand=Plus/Plus Query 210903 AGGACAAGGTGAAGAGTGGACTCTTTTTGGATGTTATAATCCGCAAGGGTCCTTCCATCT 210962 || || || || | ||||||||| || |||||||| || || | || || | |||||| Sbjct 2912628 AGCACGAGATGTAAAGTGGACTCCTTCTGGATGTTGTAGTCGGACAGAGTGCGGCCATCT 2912687 Query 210963 TCCAGCTGCTTGCCTGCGAAGATAAGGCGTTGTTGGTCAGGAGGAACTCCTTCCTTGTCC 211022 |||| |||||| || || ||||| || | || ||||| || || | ||| ||||||||| Sbjct 2912688 TCCAACTGCTTACCAGCAAAGATGAGTCTCTGCTGGTCTGGTGGGATTCCCTCCTTGTCC 2912747 Query 211023 TGGATCTTTTGTTTCACGTTTTCGATGGTGTCAGAGCTTTCAACCTCGAGAGTGATCGTC 211082 |||||||| || | || || || |||||| | |||||||| || ||||| || Sbjct 2912748 TGGATCTTGGACTTTATATTCTCTATCGTGTCACTGGGCTCAACCTCCAGGGTGATGGTT 2912807 Query 211083 TTTCCCGTGAGAGTCTTTACAAACACCTGCATTTT 211117 || || || || || || ||||||| |||||| || Sbjct 2912808 TTGCCAGTCAGGGTTTTCACAAACATCTGCATCTT 2912842 Score = 125 bits (138), Expect = 2e-23 Identities = 160/219 (73%), Gaps = 6/219 (3%) Strand=Plus/Plus Query 210902 TAGGACAAGGTGAAGAGTGGACTCTTTTTGGATGTTATAATCCGCAAGGGTCCTTCCATC 210961 |||||| || ||||| ||||||||||| |||||||| || || ||||| | |||| Sbjct 2910593 TAGGACGAGATGAAGCGTGGACTCTTTCTGGATGTTGTAGTCACACAGGGTTCGATCATC 2910652 Query 210962 TTCCAGCTGCTTGCCTGCGAAGATAAGGCGTTGTTGGTCAGGAGGAACTCCTTCCTTGTC 211021 |||||| ||||| | || || || || || || || || |||| | ||| |||||||| Sbjct 2910653 TTCCAGTTGCTTAGCAGCAAAAATGAGACGCTGCTGATCTGGAGAGATTCCCTCCTTGTC 2910712 Query 211022 CTGGATCTTTTGTTTCACGTTTTCGATGGTGTCAGAGCTT---TCAACCTCGAGAGTGAT 211078 |||||| || ||| || || | || ||||| ||| |||||||| | || || Sbjct 2910713 CTGGATTTTAGATTTTACATTCTTGAAACTGTCA---CTTGGCTCAACCTCCAATGTTAT 2910769 Query 211079 CGTCTTTCCCGTGAGAGTCTTTACAAACACCTGCATTTT 211117 ||||| || || || ||||| ||||||| |||||| || Sbjct 2910770 GGTCTTCCCGGTCAGGGTCTTAACAAACATCTGCATCTT 2910808 Score = 118 bits (130), Expect = 2e-21 Identities = 101/125 (81%), Gaps = 0/125 (0%) Strand=Plus/Minus Query 210903 AGGACAAGGTGAAGAGTGGACTCTTTTTGGATGTTATAATCCGCAAGGGTCCTTCCATCT 210962 |||||||| || || ||||||||||| |||||||| || || | ||||| | |||||| Sbjct 3994212 AGGACAAGATGGAGGGTGGACTCTTTCTGGATGTTGTAGTCAGACAGGGTGCGGCCATCT 3994153 Query 210963 TCCAGCTGCTTGCCTGCGAAGATAAGGCGTTGTTGGTCAGGAGGAACTCCTTCCTTGTCC 211022 |||||||| ||||| || ||||| || | || || ||||| |||| |||||||||||| Sbjct 3994152 TCCAGCTGTTTGCCAGCAAAGATCAGCCTCTGCTGATCAGGGGGAATGCCTTCCTTGTCC 3994093 Query 211023 TGGAT 211027 ||||| Sbjct 3994092 TGGAT 3994088 Score = 109 bits (120), Expect = 1e-18 Identities = 119/158 (75%), Gaps = 0/158 (0%) Strand=Plus/Minus Query 210957 CCATCTTCCAGCTGCTTGCCTGCGAAGATAAGGCGTTGTTGGTCAGGAGGAACTCCTTCC 211016 |||||||||||||| ||||| || ||||| || | || || ||||| |||| |||||| Sbjct 3994386 CCATCTTCCAGCTGTTTGCCAGCAAAGATCAGCCTCTGCTGATCAGGGGGAATGCCTTCC 3994327 Query 211017 TTGTCCTGGATCTTTTGTTTCACGTTTTCGATGGTGTCAGAGCTTTCAACCTCGAGAGTG 211076 ||||| ||||| || || || || || ||||||||| | |||||||| || || Sbjct 3994326 TTGTCTTGGATTTTGGCCTTTACATTCTCAATGGTGTCACTGGGCTCAACCTCAAGGGTA 3994267 Query 211077 ATCGTCTTTCCCGTGAGAGTCTTTACAAACACCTGCAT 211114 || ||||| || ||||| || || || || | |||||| Sbjct 3994266 ATGGTCTTGCCAGTGAGGGTTTTGACGAAGATCTGCAT 3994229 Score = 105 bits (116), Expect = 2e-17 Identities = 97/123 (79%), Gaps = 0/123 (0%) Strand=Plus/Minus Query 210903 AGGACAAGGTGAAGAGTGGACTCTTTTTGGATGTTATAATCCGCAAGGGTCCTTCCATCT 210962 |||||||| ||||| || || |||||||||||||| || || | || || | || ||| Sbjct 3995313 AGGACAAGATGAAGTGTAGATTCTTTTTGGATGTTGTAGTCGGAGAGAGTACGACCGTCT 3995254 Query 210963 TCCAGCTGCTTGCCTGCGAAGATAAGGCGTTGTTGGTCAGGAGGAACTCCTTCCTTGTCC 211022 |||||||| ||||| || ||||| || | || || ||||| |||| |||||||||||| Sbjct 3995253 TCCAGCTGTTTGCCAGCAAAGATCAGCCTCTGCTGATCAGGGGGAATGCCTTCCTTGTCC 3995194 Query 211023 TGG 211025 ||| Sbjct 3995193 TGG 3995191 >seq14 Length=28847931 Score = 143 bits (158), Expect = 6e-29 Identities = 160/214 (75%), Gaps = 0/214 (0%) Strand=Plus/Plus Query 210903 AGGACAAGGTGAAGAGTGGACTCTTTTTGGATGTTATAATCCGCAAGGGTCCTTCCATCT 210962 || ||||| || || |||||||| || |||||||| || || | |||||| | |||||| Sbjct 12556714 AGAACAAGATGGAGGGTGGACTCCTTCTGGATGTTGTAGTCAGAAAGGGTGCGGCCATCT 12556773 Query 210963 TCCAGCTGCTTGCCTGCGAAGATAAGGCGTTGTTGGTCAGGAGGAACTCCTTCCTTGTCC 211022 |||| ||| || || || ||||| || || || ||||| || || | ||||| || ||| Sbjct 12556774 TCCAACTGTTTACCAGCAAAGATCAGACGCTGCTGGTCTGGGGGGATACCTTCTTTATCC 12556833 Query 211023 TGGATCTTTTGTTTCACGTTTTCGATGGTGTCAGAGCTTTCAACCTCGAGAGTGATCGTC 211082 |||||||| || || ||||| ||||| ||| | || || || |||||||| ||| Sbjct 12556834 TGGATCTTGGCCTTGACATTTTCAATGGTATCACTGGGCTCCACTTCCAGAGTGATGGTC 12556893 Query 211083 TTTCCCGTGAGAGTCTTTACAAACACCTGCATTT 211116 || || || || ||||| ||||| | ||||||| Sbjct 12556894 TTGCCAGTCAGGGTCTTCACAAAGATTTGCATTT 12556927 Score = 127 bits (140), Expect = 5e-24 Identities = 157/215 (73%), Gaps = 0/215 (0%) Strand=Plus/Plus Query 210903 AGGACAAGGTGAAGAGTGGACTCTTTTTGGATGTTATAATCCGCAAGGGTCCTTCCATCT 210962 || ||||| || || | ||| || || |||||||| || || | |||||| | ||||||| Sbjct 12557398 AGAACAAGATGGAGGGGGGATTCCTTCTGGATGTTGTAGTCAGAAAGGGTGCGTCCATCT 12557457 Query 210963 TCCAGCTGCTTGCCTGCGAAGATAAGGCGTTGTTGGTCAGGAGGAACTCCTTCCTTGTCC 211022 || || ||||| || || ||||| || || || || || || || | ||||| || ||| Sbjct 12557458 TCAAGTTGCTTACCAGCAAAGATCAGACGCTGCTGATCTGGGGGGATACCTTCTTTTTCC 12557517 Query 211023 TGGATCTTTTGTTTCACGTTTTCGATGGTGTCAGAGCTTTCAACCTCGAGAGTGATCGTC 211082 |||||||| || || ||||| ||||| ||| | || || || |||||||| | | Sbjct 12557518 TGGATCTTGGCCTTGACATTTTCAATGGTATCACGGGGCTCCACTTCCAGAGTGATGGCC 12557577 Query 211083 TTTCCCGTGAGAGTCTTTACAAACACCTGCATTTT 211117 || || || || ||||| ||||| | ||||| || Sbjct 12557578 TTGCCAGTCAGGGTCTTCACAAAGATTTGCATCTT 12557612 >seq15 Length=20091810 Score = 98.7 bits (108), Expect = 2e-15 Identities = 140/194 (72%), Gaps = 6/194 (3%) Strand=Plus/Minus Query 139822 GGAAGAGGAGGACAATCTGCTCTGCGTTTTGAAAGACTTCGAAAGGAAAAGAGGCACAAC 139881 |||||||||||||| |||||||| ||||||| | || ||| ||| ||||| |||||| Sbjct 9546545 GGAAGAGGAGGACAGTCTGCTCTTCGTTTTGCTCGTCTGAGAATGGAGAAGAGACACAAC 9546486 Query 139882 TACTTGAGAAAAGTTGCAGAGAACGCAATACAGTGTTTTAT---CAAAGACAACAAGTGT 139938 || | |||||||| || |||| || | ||| ||| | ||| |||| |||| Sbjct 9546485 TATGTTAGAAAAGTGGCTGAGACGGCTGTGCAGCTCTTTGTGTCCAACGACA--AAGT-C 9546429 Query 139939 TTTGTTTCTGGTATTGTTTTGGCAGGTTCCGCAGAGTTCAAGACAAAGCTCCTGGAATCT 139998 |||| | || || || ||||| ||||| || || || ||||| |||| ||||| Sbjct 9546428 AATGTTGCAGGAATGGTCTTGGCTGGTTCGGCTGACTTTAAGACTGAGCTGAGCCAATCT 9546369 Query 139999 GACTTTTTTGACCC 140012 ||| | |||||||| Sbjct 9546368 GACATGTTTGACCC 9546355 Lambda K H 0.634 0.408 0.912 Gapped Lambda K H 0.625 0.410 0.780 Effective search space used: 1158711752983695