Alignment viewer

BLASTN 2.6.0+ Reference: Stephen F. Altschul, Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Database: Homo_20sapiens_20[refseq_20GCF_5F000001405.37_202017-06-14] 578 sequences; 3,253,848,404 total letters Query= NC_003391.1 Length=205719 Score E Sequences producing significant alignments: (Bits) Value seq64 361 7e-95 seq31 163 4e-35 seq1 154 2e-32 seq11 114 2e-20 seq29 111 2e-19 seq51 89.7 7e-13 >seq64 Length=156040895 Score = 361 bits (400), Expect = 7e-95 Identities = 646/938 (69%), Gaps = 20/938 (2%) Strand=Plus/Plus Query 35811 TTTAAAAATCAACATCTAGAGAAAAATGATTGTCTTCTTGA---GACATAACTCCCATTT 35867 |||| || ||| ||| || | |||| ||| |||| | || |||| |||||||| Sbjct 44309313 TTTAGAAGTCAGCATTTAAGGTAAAAGAATTCTCTTTTGGACTTGACACCACTCCCATCC 44309372 Query 35868 TTTGGTATTCACCCACACGTTTTTCGAAAAAATTAGTTTTACCTTCTAATGATATATTTT 35927 | || || || || || | | || || || ||||| || | | ||| ||||| | Sbjct 44309373 TCTGATACTCGCCTACTCTCCTCTCAAAGAAGTTAGTCTTTC-----AGTGAAATATTCT 44309427 Query 35928 CCATGAAATCGAATGGATTGATAACATTATAAAtttttttAAATCCCAATTCAGAAATCA 35987 || | || || ||||| | || ||| || |||| |||| ||| || | Sbjct 44309428 CCGTAAAGTCAAATGGGCTCTCTACTCCGAAAACCTTGCTAAAACCCAGTTCCAGCATAA 44309487 Query 35988 ATCTATCTGCGACAAATTCTATATATGTTTTCATCATTTCACAATTCATTCCTATAAGTT 36047 ||| ||||| ||||| || || ||| ||||| | |||||||| || || || | Sbjct 44309488 GTCTGTCTGCCACAAACTCAATGTATTGCTTCATTAGAGTGCAATTCATCCCAATGAGCT 44309547 Query 36048 TAACTGGAAGAGCCACAGTAAGAAATTCTTGTTCAATAGAGACCGCGTCTGTTATAATAG 36107 | || || | ||| | || || ||||||||||| || || || | | || || Sbjct 44309548 TCACAGGCAAGGCCTCGGTGAGGAATTCTTGTTCTATCTGAACAGCATTGATAATTATTT 44309607 Query 36108 ATATTACTGTTTCTTCACTCGGTGGATACAATAAATGTTTAAACATCAAACATGCGAAAT 36167 | | ||| | || || ||| | | | ||||| |||||||| || || |||| Sbjct 44309608 CTCTCACTTTCTCCTCCGATGGTTTGTGTACCAGGTGTTTGAACATCAAGCAAGCAAAAT 44309667 Query 36168 CGCAGTGTAGACCCTCGTCTCTACTAATTAATTCGTTGGAAAACGTGAGTCCGGGCATTA 36227 | ||||||| |||||| ||||| ||||| | ||| |||||||||||||| || ||||| Sbjct 44309668 CACAGTGTAAACCCTCTTCTCTGCTAATAAGTTCATTGGAAAACGTGAGGCCAGGCATCG 44309727 Query 36228 GGCCACGCTTTTTAAGCCAAAATATGGACGCAAATGATCCAGAAAAGAAAATTCCTTCTA 36287 | || || || || ||||| ||||| || ||||| || ||||||||||| || ||||||| Sbjct 44309728 GTCCTCGTTTCTTGAGCCAGAATATCGATGCAAAAGAACCAGAAAAGAAGATGCCTTCTA 44309787 Query 36288 CTGCCGCAAAGGCAATAAGTCTCTCTCCATA---ACCGGCACTGTCATGTATCCACTTTT 36344 |||| |||||||| | || | || ||||| | | | |||| ||||| Sbjct 44309788 CTGCAGCAAAGGCTACAACACGTTCACCATAGGTAGCCTCTTTGTCCCCAATCCAGCGCA 44309847 Query 36345 GAGCCCAATCGGCCTTCTTTTTTACACAAGGCATTGTCTCTATAGCATTAAAGAGATAGt 36404 |||||||| |||||||| || ||||||||||| || || || ||||| |||||| | | Sbjct 44309848 AGGCCCAATCTGCCTTCTTCTTGACACAAGGCATCGT-TCAATGGCATTGAAGAGAAATT 44309906 Query 36405 ttttttCATTACTATCTTTAACATATGTATCAATCAAAAGACTATACATTTCCGAATGAA 36464 |||| || |||||| | || || ||||| | |||||||||||||| ||||| | Sbjct 44309907 CCCTTTCTTTGGGATCTTTGATGTAAGTGTCAATAAGGAGACTATACATTTCAGAATGTA 44309966 Query 36465 TGTTTTCAATGGCCATTTGAAATCCGTAGAAACATCTAGCCTCGGTAATCTGTACTTCTG 36524 ||||||| ||||| ||||| || || |||||||| | || || |||||||| ||| | Sbjct 44309967 TGTTTTCCATGGCAATTTGGAAGCCATAGAAACAGCGGGCTTCTGTAATCTGAACTATTT 44310026 Query 36525 TACAAAATCGTTCTGCTAAATTTTCATTCACTATTCCGTCACTGGCTGCAAAAAACGCCA 36584 | |||||| || | ||||||||||| | ||| || ||||| |||||||| || ||| Sbjct 44310027 GGCTAAATCGCTCCACCAAATTTTCATTTATTATGCCATCACTTGCTGCAAAGAAAACCA 44310086 Query 36585 ATACATGTTTTATAAAATATTTTTCGTTTGGTGTTAGTTTATTCCAATCATTG----ATA 36640 ||||| |||||||||| | || | || | || ||||| || || || Sbjct 44310087 GAACATGGGATATAAAATATCTCTCCTCGGGCTTCAG-GGATTCC---CAGTGCCGAATG 44310142 Query 36641 TCTTTAGATATATCCACTTCTTCCACTGTCCAAAATGATGCCTCTGCCTTTTTATACATA 36700 || || || | ||||| || || ||||| || || ||||| ||||| | |||||| Sbjct 44310143 TCCTTGGACAGGTCCACCTCCTCGGTGGTCCAGAAGGAAGCCTCCGCCTTCTCATACATC 44310202 Query 36701 TTCCAGATGTCATGATATTGAATTGGAAAAATAACAAA 36738 | |||||| ||||| || | || || || || ||||| Sbjct 44310203 TGCCAGATATCATGGTACTCGATGGGGAAGATGACAAA 44310240 Score = 203 bits (224), Expect = 4e-47 Identities = 382/554 (69%), Gaps = 12/554 (2%) Strand=Plus/Minus Query 36170 CAGTGTAGACCCTCGTCTCTACTAATTAATTCGTTGGAAAACGTGAGTCCGGGCATTAGG 36229 ||||||| |||||| || || ||||| | ||| |||||||| ||||| || ||||| || Sbjct 144309680 CAGTGTAAACCCTCATCACTGCTAATAAGTTCATTGGAAAATGTGAGGCCAGGCATCAGT 144309621 Query 36230 CCACGCTTTTTAAGCCAAAATATGGACGCAAATGATCCAGAAAAGAAAATTCCTTCTACT 36289 || | || || ||||| ||||| | ||||| || ||||||||||| | | ||| || Sbjct 144309620 CCTTGTTTCTTGAGCCAGAATATCAATGCAAAAGAACCAGAAAAGAAGGTGCTTTCCACC 144309561 Query 36290 GCCGCAAAGGC--AAT--AAGTCTCTCTCCAT---AACCGGCACTGTCATGTATCC-ACT 36341 |||||||| || || | ||||||| | || | ||| |||| || Sbjct 144309560 AGGGCAAAGGCTGCATGCAACACGTTCTCCATGGGTAACGTCTTTGTTCCCAATCCAACA 144309501 Query 36342 TTTGAGCCCAATCGGCCTTCTTTTTTACACAAGGCATTGTCTCTATAGCATTAAAGAGAT 36401 || ||||| | |||||||| || |||| |||||| || || || ||| | |||||| Sbjct 144309500 CAAGA-CCCAACCTGCCTTCTTCTTGACAC-AGGCATCGTTTCAATGGCACTGAAGAGAA 144309443 Query 36402 AGtttttttCATTACTATCTTTAACATATGTATCAATCAAAAGACTATACATTTCCGAAT 36461 | | |||| || |||||||| |||||| ||||| | ||| ||| ||||| |||| Sbjct 144309442 ATTCCCTTTCTTTGTTATCTTTA--ATATGTGTCAATAAGGAGATTATTTATTTCAGAAT 144309385 Query 36462 GAATGTTTTCAATGGCCATTTGAAATCCGTAGAAACATCTAGCCTCGGTAATCTGTACTT 36521 | |||||||| || || ||||| || || ||||| || | || |||||||| |||| Sbjct 144309384 GTATGTTTTCTATAGCAATTTGGAAGCCATAGAAGCAGAAGGTTTCTGTAATCTGAACTT 144309325 Query 36522 CTGTACAAAATCGTTCTGCTAAATTTTCATTCACTATTCCGTCACTGGCTGCAAAAAACG 36581 || | |||| |||| | || ||||||| | ||| || |||| |||||||| || | Sbjct 144309324 CTTGAACAAATTGTTCCACCAAGTTTTCATCTATTATCCCATCACATGCTGCAAAGAAAG 144309265 Query 36582 CCAATACATGTTTTATAAAATATTTTTCGTTTGGTGTTAGTTTATTCCAATCATTGATAT 36641 ||| | ||| |||||||||| ||| | || | || | ||| | || | Sbjct 144309264 CCAGAATATGGGATATAAAATATCTTTACTCAGGCTTCAGGGCTTCCCAGTGCCAAATGT 144309205 Query 36642 CTTTAGATATATCCACTTCTTCCACTGTCCAAAATGATGCCTCTGCCTTTTTATACATAT 36701 | || || | ||||| || | | |||| || || ||||| | || || ||||| | Sbjct 144309204 CCTTGGACAGGTCCACCTCCTGGGCCATCCAGAAAGAAACCTCTACTTTCTTTTACATTT 144309145 Query 36702 TCCAGATGTCATGA 36715 |||| || |||||| Sbjct 144309144 TCCAAATATCATGA 144309131 >seq31 Length=133275309 Score = 163 bits (180), Expect = 4e-35 Identities = 159/204 (78%), Gaps = 3/204 (1%) Strand=Plus/Plus Query 9218 TTTTAAACTTTATTTCTTATTAGATACTTCCAAAAGCTGTAACAGATGCAAGAACAGATT 9277 |||||| ||| || |||||| | |||||| || | ||||||| |||| ||| ||||| Sbjct 66137954 TTTTAA---TTACTTTTTATTAACTGCTTCCAGAAACCGTAACAGGTGCAGGAATAGATT 66138010 Query 9278 GATGATATCCAAGTAGAAATTGATAGAGGCTAACACATAGTCTTCAGGCGAGAGCTTATG 9337 ||||||||||||||||| |||| | ||||| || || |||||||| || || ||||| Sbjct 66138011 GATGATATCCAAGTAGAGGCTGATGGCAGCTAATACGTACTCTTCAGGTGACAGTTTATG 66138070 Query 9338 TATTAGTGAGTGCGTGTCATAGATAATGAATCCGCAGAATACAAGGGCCCCAAAAGCAGA 9397 || |||||||| ||||||||||| |||||||| ||||| | |||||| || ||| | Sbjct 66138071 CATCAGTGAGTGTGTGTCATAGATGATGAATCCACAGAAAAGAAGGGCTCCTGCAGCGGC 66138130 Query 9398 CAGGACCAGCTCCACTGTTTCACT 9421 | ||||| ||||| | | ||||| Sbjct 66138131 TAAGACCAACTCCATTATCTCACT 66138154 Score = 129 bits (142), Expect = 8e-25 Identities = 100/119 (84%), Gaps = 0/119 (0%) Strand=Plus/Plus Query 9480 CCTGCTCCAAGTTTACTGAAATCTCTCTTTGATTGTAGAGTATATGCAGTCAGAGCAAGA 9539 |||||||||| ||| |||||||| |||| |||||||||||||| |||||| | ||| | Sbjct 66145841 CCTGCTCCAAATTTGCTGAAATCCTTCTTAGATTGTAGAGTATACACAGTCAAACCAAAA 66145900 Query 9540 AACACTGCAGTAGTCAGCATGAAAGCTTGCATAACGACATGCACATCATAGAAAGTAAC 9598 || |||| ||||||||| ||||||||||||| || | || ||||||||||||||||| Sbjct 66145901 AATACTGTAGTAGTCAGTATGAAAGCTTGCAGAATAATATATACATCATAGAAAGTAAC 66145959 Score = 84.2 bits (92), Expect = 3e-11 Identities = 60/69 (87%), Gaps = 0/69 (0%) Strand=Plus/Plus Query 9847 CCATTCGTATGTGCACACTGGCAGACGCCACCGAGCTGCCATAGTTGAAGTCGTCCTCGA 9906 ||||||| |||||||| |||| || ||||| |||||||||||||||||||||||||| Sbjct 66169855 CCATTCGGATGTGCACGGTGGCGGAGGCCACGCTGCTGCCATAGTTGAAGTCGTCCTCGA 66169914 Query 9907 TGGAGGAGC 9915 | ||||||| Sbjct 66169915 TCGAGGAGC 66169923 >seq1 Length=248956422 Score = 154 bits (170), Expect = 2e-32 Identities = 237/336 (71%), Gaps = 9/336 (3%) Strand=Plus/Plus Query 36270 AAAAGAAAATTCCTTCTACTGCCGCAAAGGCAATAAGTCTCTCTCCATA---ACCGGCAC 36326 ||||||| || |||| ||||| |||||||| | || | || ||||| | | | Sbjct 161378705 AAAAGAAGATATCTTCCACTGCAGCAAAGGCTACAACACATTCACCATAGGTAGCCTCTT 161378764 Query 36327 TGTCATGTATCCACTTTTGAGCCCAATCGGCCTTCTTTTTTACACAAGGCATTGTCTCTA 36386 |||| || || | ||||||| |||||||| |||||||| ||| || | Sbjct 161378765 TGTCCCCAATACAACTCAAGGCCCAATTTGCCTTCTTG---ACACAAGG---TGTTTCAA 161378818 Query 36387 TAGCATTAAAGAGATAGtttttttCATTACTATCTTTAACATATGTATCAATCAAAAGAC 36446 | | || |||||| | | |||| || |||||| | || || ||||| | |||| Sbjct 161378819 TGGTGTTGAAGAGAAATTCCCTTTCTTTGGCATCTTTTATGTAAGTGTCAATAAGGAGAC 161378878 Query 36447 TATACATTTCCGAATGAATGTTTTCAATGGCCATTTGAAATCCGTAGAAACATCTAGCCT 36506 |||||||||| ||||| ||||||| ||||| ||||| || | |||||||| | || | Sbjct 161378879 TATACATTTCAGAATGTGTGTTTTCCATGGCAATTTGGAAGTCATAGAAACAGCGGGCTT 161378938 Query 36507 CGGTAATCTGTACTTCTGTACAAAATCGTTCTGCTAAATTTTCATTCACTATTCCGTCAC 36566 | |||||||| |||||| | |||||| || | || |||||||| ||||| || |||| Sbjct 161378939 CTGTAATCTGAACTTCTTGGCTAAATCGCTCCACCAAGTTTTCATTTACTATGCCATCAC 161378998 Query 36567 TGGCTGCAAAAAACGCCAATACATGTTTTATAAAAT 36602 | |||||||| || |||| ||| | |||||||| Sbjct 161378999 TTGCTGCAAAGAAAGCCAGAACACGGGATATAAAAT 161379034 >seq11 Length=242193529 Score = 114 bits (126), Expect = 2e-20 Identities = 98/121 (81%), Gaps = 0/121 (0%) Strand=Plus/Minus Query 36180 CCTCGTCTCTACTAATTAATTCGTTGGAAAACGTGAGTCCGGGCATTAGGCCACGCTTTT 36239 |||| ||||| ||||| | ||| || ||||| ||||| || ||||| || || || || | Sbjct 10127221 CCTCATCTCTGCTAATAAGTTCATTAGAAAATGTGAGGCCAGGCATCAGTCCTCGTTTCT 10127162 Query 36240 TAAGCCAAAATATGGACGCAAATGATCCAGAAAAGAAAATTCCTTCTACTGCCGCAAAGG 36299 | ||||| ||||| |||||||| || || ||||||||||| ||||| ||||| ||||||| Sbjct 10127161 TGAGCCAGAATATCGACGCAAAAGAACCGGAAAAGAAAATGCCTTCCACTGCAGCAAAGG 10127102 Query 36300 C 36300 | Sbjct 10127101 C 10127101 Score = 104 bits (114), Expect = 3e-17 Identities = 95/120 (79%), Gaps = 0/120 (0%) Strand=Plus/Minus Query 36418 ATCTTTAACATATGTATCAATCAAAAGACTATACATTTCCGAATGAATGTTTTCAATGGC 36477 |||||| | || || ||||| | ||||||||||||||| ||||| |||||||| ||||| Sbjct 10124839 ATCTTTTATGTAAGTGTCAATAAGAAGACTATACATTTCAGAATGTATGTTTTCCATGGC 10124780 Query 36478 CATTTGAAATCCGTAGAAACATCTAGCCTCGGTAATCTGTACTTCTGTACAAAATCGTTC 36537 ||||| || || |||||||| | || || |||||||| |||||| | |||||| || Sbjct 10124779 AATTTGGAAGCCATAGAAACAGCGGGCTTCTGTAATCTGAACTTCTTGGCTAAATCGCTC 10124720 >seq29 Length=135086622 Score = 111 bits (122), Expect = 2e-19 Identities = 127/171 (74%), Gaps = 0/171 (0%) Strand=Plus/Minus Query 64906 TCCATCCGTAAAAATGCATACTCGTTAGTTTGGAATAACTCGGATCTGCTATATGGATAT 64965 |||| ||||| || |||||||| || |||||| ||| | || || || || ||||| | Sbjct 4135265 TCCAGCCGTAGAAGTGCATACTAGTGAGTTTGCCATAGTTAGGCTCAGCAATGTGGATGT 4135206 Query 64966 TCATAGATTGACTTTGATCGATGAAGGCTCCCCTGTCTGCAGCCATTTTTATGATCGTCT 65025 ||| |||||| |||||||| ||||| || || || || || ||||| || | | || | Sbjct 4135205 TCAAAGATTGGCTTTGATCAATGAAAGCACCTCTCTCAGCTGCCATCTTGAGAACAGTTT 4135146 Query 65026 TTTGTGGAATTTCCCAAATAGTTTTATAAACTCGCTTAATATCTTCTGGAA 65076 | || | |||||||| | ||||||||||| | |||| | || || |||| Sbjct 4135145 TCTGAGAGATTTCCCACACAGTTTTATAAAGTTGCTTCAGGTCATCAGGAA 4135095 Score = 107 bits (118), Expect = 3e-18 Identities = 106/137 (77%), Gaps = 0/137 (0%) Strand=Plus/Minus Query 65181 ACTTGAAATTCTCCAGACAATACTCTCCGAGTGTAAATATTACTGGTATACGGTTCCACA 65240 || ||||||||||| ||||| ||||| ||||| || || || ||||| || ||||| | Sbjct 4132423 ACCTGAAATTCTCCTGACAAGACTCTGCGAGTATAGATGTTGCTGGTGTAAGGTTCAATG 4132364 Query 65241 GACTCATTATTTCCCAAAATTTGAGCAGTTGATGCAGTCGGCATAGGTGCCACCAATAAA 65300 ||||||||||| |||| || |||||||| || || || ||||| || || | | |||| Sbjct 4132363 GACTCATTATTCCCCAGGATCTGAGCAGTGGAAGCTGTAGGCATCGGGGCAATAAGTAAA 4132304 Query 65301 CTATTTCTAAGACCGTA 65317 || ||||| | ||| || Sbjct 4132303 CTGTTTCTTATACCATA 4132287 Score = 100 bits (110), Expect = 4e-16 Identities = 105/138 (76%), Gaps = 0/138 (0%) Strand=Plus/Minus Query 66056 CTTCACTCGTTTCATAAAGAGATCAGGAATCCAAAGAGCTATAAATAAATCTCTGGTTCT 66115 || |||||||||||| |||||||| |||||||||||||| | || | |||||||| | Sbjct 4121756 CTCCACTCGTTTCATGAAGAGATCCGGAATCCAAAGAGCAAAGAAAAGATCTCTGGCACG 4121697 Query 66116 ATGTTCCTCGTTTCCTGTATTCTTTTTAAGATCGAGGAACGCCATAATATCATAATGCCA 66175 || || || |||||||| ||||| || | ||| ||||| | | || || ||||||| Sbjct 4121696 CTGCTCTTCCTTTCCTGTGTTCTTCTTTAAATCAAGGAATTCAAAGATGTCTAAATGCCA 4121637 Query 66176 CGGTTCCAAGTATATGGC 66193 || |||| ||| || || Sbjct 4121636 AGGCTCCAGGTAAATAGC 4121619 Score = 84.2 bits (92), Expect = 3e-11 Identities = 118/166 (71%), Gaps = 0/166 (0%) Strand=Plus/Minus Query 66799 CACTAAATAGtttttttGTTTCTTTGTGTAGATTTGATACGGCTATTCTAGCGGCTATAA 66858 |||| || | ||| |||||||||||||| | || || || || || || || || | | Sbjct 4106223 CACTGAACACTTTCTTTGTTTCTTTGTGCAAGTTAGAGACAGCGATCCTGGCTGCCAGGA 4106164 Query 66859 TGGCATAATCCGGATGTTGTGTAGTACAAGTGGCTGCTATTTCGGCTGCCAGAGTGTCCA 66918 | ||||| || || || | ||||| | || || ||| |||| || |||| ||| || | Sbjct 4106163 TAGCATAGTCAGGGTGCTTAGTAGTCAAGGTTGCAGCTGTTTCAGCAGCCAAAGTATCTA 4106104 Query 66919 ATTCTACCGTTGTTACTCCATTATATATTCCTTGGATAACCTTCAT 66964 ||| || || || || ||| | || | |||||||| || ||||| Sbjct 4106103 GTTCCACTGTGGTGACCCCACTGTACAAGCCTTGGATTACTTTCAT 4106058 >seq51 Length=58617616 Score = 89.7 bits (98), Expect = 7e-13 Identities = 179/259 (69%), Gaps = 10/259 (4%) Strand=Plus/Minus Query 33900 TCCGGTTGATCCAAACCCT--TGATCTCCTCTATCTGTACTATCTAGAGATTGTACTTCT 33957 |||||| || ||||| ||| ||||| ||| | ||| ||| | || ||| |||||| Sbjct 52451934 TCCGGTAGAGCCAAAACCTCCTGATCCCCTTTTGGTGTC--ATCCAAAGCTTGAACTTCT 52451877 Query 33958 TTCAGTTCTGGAT-AATATATACGTTGATAGATTAGCTGAGCTATTCTATCCCCCGTATT 34016 | | |||||||| || | || |||| | | || ||||| || |||| ||| || | || Sbjct 52451876 TCTATTTCTGGATTAAAAAATCCGTTCACAAATGAGCTGTGCAATTCGATCACCTTTTTT 52451817 Query 34017 T-ACATTGAATGTACATTTTCCATTATTAATAAGAATGACTCCTATATTTCCCCTATAAT 34075 | || | || | ||| | | ||||| || | || || | |||||| ||||||| Sbjct 52451816 TGACTTCAAACTTTTCTTTGCAAAAATTAAAGAGTACAACACCAACATTTCCTCTATAAT 52451757 Query 34076 CTTCGTCTATTACACCGCCTCCTATATCAATA---CCTTTTAGGGACAGACCAGACCTAG 34132 |||| ||| | | ||| |||||| ||||| | |||| | || |||||||| | Sbjct 52451756 CTTCATCTGTGATACCAGCTCCTACATCAACAAAGTGTTTTGCAGCCAAGCCAGACCTTG 52451697 Query 34133 GAGCTATTCTACCATAGCA 34151 |||||| ||| ||||| || Sbjct 52451696 GAGCTACTCT-CCATAACA 52451679 Lambda K H 0.634 0.408 0.912 Gapped Lambda K H 0.625 0.410 0.780 Effective search space used: 658168044701520