Alignment viewer
BLASTN 2.6.0+
Reference: Stephen F. Altschul, Thomas L. Madden, Alejandro A.
Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J.
Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of
protein database search programs", Nucleic Acids Res. 25:3389-3402.
Database: Raphanus_20sativus_20[refseq_20GCF_5F000801105.1_202015-09-29]
10,676 sequences; 426,614,037 total letters
Query= NC_019516.1
Length=161440
Score E
Sequences producing significant alignments: (Bits) Value
seq10676 679 0.0
seq3905 286 4e-72
seq3 224 1e-53
seq722 104 2e-17
seq3442 96.9 4e-15
seq68 91.5 2e-13
seq7 84.2 2e-11
>seq10676
Length=153368
Score = 679 bits (752), Expect = 0.0
Identities = 727/957 (76%), Gaps = 8/957 (1%)
Strand=Plus/Minus
Query 8290 GGCTGGTTTGGCGTTCTCATGATCCCAACCCTTCTTG-CCGCTACAATTTGC---TTCAT 8345
|| |||||||| ||| | ||||| || ||| || ||| |||| || || | || ||
Sbjct 1284 GGATGGTTTGGTGTTTTGATGATTCCTACC-TTATTGACCGCAAC---TTCCGTTTTTAT 1229
Query 8346 TACCGCATTCGTTGCTGCTCCTCCTGTGGACATCGACGGCATCCGCGAACCAGTTGCTGG 8405
|| ||||||| ||||||||||||| || || || || || || || ||||| ||| ||||
Sbjct 1228 TATCGCATTCATTGCTGCTCCTCCAGTAGATATTGATGGTATTCGTGAACCTGTTTCTGG 1169
Query 8406 ATCTCTCATGTACGGTAACAACATCATCTCTGGTGCAGTTGTTCCCAGCTCTAACGCAAT 8465
|||||| | ||||| ||||| || || || ||||| || |||| | ||| || ||
Sbjct 1168 ATCTCTTCTTTACGGAAACAATATTATTTCAGGTGCCATTATTCCTACTTCTGCAGCTAT 1109
Query 8466 TGGCCTCCACTTCTACCCCATCTGGGAAGCCGCTTCTCTTGATGAGTGGCTCTACAACGG 8525
||| | || || || || ||||||||||| || || ||||||| ||||| ||||||||
Sbjct 1108 TGGTTTGCATTTTTATCCGATCTGGGAAGCTGCATCCGTTGATGAATGGCTATACAACGG 1049
Query 8526 TGGTCCTTACCAGCTTGTAGTTTTCCACTTCCTCCTTGGCGTCTTTGCTTATATGGGTCG 8585
||||||||| | || | ||| | ||||| | ||||| || | ||||||||||||
Sbjct 1048 TGGTCCTTATGAACTAATTGTTCTACACTTTTTACTTGGTGTAGCTTGTTATATGGGTCG 989
Query 8586 TGAATGGGAACTTTCTTACCGCCTTGGCATGCGTCCTTGGATCTGTGTTGCATACTCTGC 8645
||| ||||||||| || ||| || || |||||||||||||| ||||||||| || ||
Sbjct 988 TGAGTGGGAACTTAGTTTCCGTCTGGGTATGCGTCCTTGGATTGCTGTTGCATATTCAGC 929
Query 8646 TCCTGTTGCCGCTGCTTCTGCTGTATTCCTTGTCTATCCTTTTGGTCAAGGTTCGTTCTC 8705
||||||||| |||||| ||||||| ||| | |||| || |||||||||| || ||
Sbjct 928 TCCTGTTGCAGCTGCTACTGCTGTTTTCTTGATCTACCCAATTGGTCAAGGAAGTTTTTC 869
Query 8706 TGATGGTATGCCTCTTGGTATCTCCGGTACGTTCAACTATATGCTTGTCTTCCAGGCTGA 8765
||||||||||||||| || ||||| ||||| ||||||| |||| |||| |||||||||||
Sbjct 868 TGATGGTATGCCTCTAGGAATCTCTGGTACTTTCAACTTTATGATTGTATTCCAGGCTGA 809
Query 8766 GCACAACATCCTGATGCACCCCTTCCACATGCTTGGTGTTGCTGGTGTCTTCGGCGGATC 8825
||||||||| || |||||||| || |||||| | ||||| |||||||| |||||||| ||
Sbjct 808 GCACAACATTCTTATGCACCCATTTCACATGTTAGGTGTAGCTGGTGTATTCGGCGGCTC 749
Query 8826 TCTCTTTTCCGCAATGCATGGTTCACTCGTTACTTCTTCGCTGGTTCGTGAGACCACCGA 8885
|| ||| || ||||||||||| | || |||||| || | | || ||||| ||
Sbjct 748 CCTATTTAGTGCTATGCATGGTTCTTTGGTAACTTCTAGTTTGATCAGGGAAACCACAGA 689
Query 8886 AACCGAGTCTCAAAACTATGGCTACAAGTTCGGTCAAGAAGAGGAAACTTACAACATTGT 8945
|| || ||| || | || |||| |||||||||||||| |||||||||||||||||
Sbjct 688 AAATGAATCTGCTAATGAAGGGTACAGATTCGGTCAAGAAGAAGAAACTTACAACATTGT 629
Query 8946 TGCCGCTCATGGCTACTTTGGTCGCTTGATCTTCCAATATGCATCTTTTAACAATTCTAG 9005
|| ||||| || || ||||| || ||||||||||||||||| ||| ||||||||| |
Sbjct 628 AGCTGCTCACGGTTATTTTGGCCGATTGATCTTCCAATATGCTAGTTTCAACAATTCTCG 569
Query 9006 AAGCTTGCATTTCTTCCTCGCTGCGTGGCCTGTGGTGGGAATCTGGTTTACCGCCCTCGG 9065
|| ||||||||| | || || ||||| || || || || |||||||| || | ||
Sbjct 568 TTCTTTACATTTCTTCTTAGCGGCTTGGCCGGTAGTAGGTATTTGGTTTACTGCTTTAGG 509
Query 9066 CGTCTCGACCATGGCTTTCAACCTCAATGGCTTCAACTTCAACCAGTCCATTATCGACGG 9125
| || |||||||||||||| ||||| ||||| |||||||| || | | || |
Sbjct 508 TATTAGTACTATGGCTTTCAACCTAAATGGTTTCAATTTCAACCAATCAGTAGTTGATAG 449
Query 9126 ACAAGGACGAGTGCTCAACACCTGGGCAGACGTACTTAACCGCGCAGGACTCGGTATGGA 9185
|||||||| || | || || ||||| || | ||||||| || || ||||||||
Sbjct 448 TCAAGGACGTGTTATTAATACTTGGGCTGATATTATTAACCGTGCTAACCTTGGTATGGA 389
Query 9186 AGTCATGCACGAAAGAAACGCACACAACTTCCCGCTTGACCTTGCGGCTGCTGAGTC 9242
||| ||||| ||| | || || ||||||||||| || ||||| || |||| ||||||
Sbjct 388 AGTTATGCATGAACGTAATGCTCACAACTTCCCTCTAGACCTAGCTGCTGTTGAGTC 332
Score = 578 bits (640), Expect = 4e-160
Identities = 727/998 (73%), Gaps = 0/998 (0%)
Strand=Plus/Plus
Query 9815 TTTGACATCCTTGATGACTGGCTTAAACGAGATCGCTTTGTTTTTGTGGGTTGGTCTGGA 9874
||||| || | ||||||||| | | || ||||| |||||||| |||||||||||
Sbjct 31980 TTTGATATTATGGATGACTGGTTACGGAGGGACCGCTTCGTTTTTGTAGGTTGGTCTGGT 32039
Query 9875 CTACTTCTTTTTCCCACTGCTTATCTTGCAATTGGTGGCTGGCTTACTGGCACTACGTTT 9934
||| | || ||||| ||| ||| | || | || || ||| | || || || || |||
Sbjct 32040 CTATTGCTCTTTCCTTGTGCCTATTTCGCTTTGGGGGGTTGGTTCACAGGTACAACCTTT 32099
Query 9935 GTCACGAGTTGGTACACTCACGGACTTGCAAGTAGTTACCTTGAGGGTGCTAATTTCCTT 9994
|| || ||||| ||||| ||| | || ||| || | || ||| ||||| |
Sbjct 32100 GTAACTTCATGGTATACTCATGGATTGGCTAGTTCCTATTTAGAAGGTTGCAATTTTTTA 32159
Query 9995 ACAGCGGCTGTCTCAACGCCTGCTGATGCTATGGGTCATTCTCTTCTTCTACTTTGGGGT 10054
|| || || || || || |||||| || | | | |||||| | | |||| ||||||
Sbjct 32160 ACCGCTGCAGTTTCTACTCCTGCTAATAGTTTAGCGCATTCTTTGTTGTTACTGTGGGGT 32219
Query 10055 CCTGAGTCTCAGGGAGATTTCGTCCGCTGGATCCAGCTTGGGGGACTCTGGGCTTTTGTG 10114
||||| | || |||||||| || ||| || | || || || |||||||||||
Sbjct 32220 CCTGAAGCACAAGGAGATTTTACTCGTTGGTGTCAATTAGGCGGTCTGTGGGCTTTTGTT 32279
Query 10115 GCGCTCCACGGAGCCTTCGCTTTAATCGGCTTTATGTTACGCCAGTTTGAGCTGGCGAGA 10174
|| |||||||| || ||||| ||||| || ||||||||||| || ||||| || || ||
Sbjct 32280 GCTCTCCACGGTGCTTTCGCATTAATAGGTTTTATGTTACGTCAATTTGAACTTGCTCGA 32339
Query 10175 TTAATTGGAATCCGACCTTACAATGCTATCGCGTTTTCTGGGCCTATTGCTGTTTTTGTC 10234
| || | | |||||||| ||||| ||||| || ||||| || ||||||||||||||
Sbjct 32340 TCTGTTCAATTGCGACCTTATAATGCAATCGCATTCTCTGGTCCAATTGCTGTTTTTGTT 32399
Query 10235 AGTGTTTTCCTCATCTATCCACTCGGACAGTCTAGTTGGTTCTTTGCGCCGAGTTTCGGC 10294
||| || || || |||||||| || || ||| |||||||||||||||| ||||| ||
Sbjct 32400 TCTGTCTTTCTAATTTATCCACTAGGTCAATCTGGTTGGTTCTTTGCGCCTAGTTTTGGT 32459
Query 10295 GTCGCGGCGATTTTCCGCTTCCTTCTCTTCCTCCAGGGCTTTCATAATTGGACGCTCAAT 10354
|| ||||| || || || ||| | ||||| |||| || |||||||||||||| | ||
Sbjct 32460 GTAGCGGCTATATTTCGATTCATCCTCTTTTTCCAAGGGTTTCATAATTGGACATTGAAC 32519
Query 10355 CCCTTCCATATGATGGGAGTTGCTGGTATACTGGGAGGGGCGCTCCTATCAGCAATCCAC 10414
|| || |||||||||||||| |||||| ||||||| | ||| || ||| || || ||
Sbjct 32520 CCATTTCATATGATGGGAGTCGCTGGTGTACTGGGCGCGGCTCTGTTATGCGCTATTCAT 32579
Query 10415 GGAGTCACTGTAGAAAACACACTTTATCAAGATGGCGAACAAGCAAACACTTTCAAAGCT 10474
|| | ||||||||||| || | | | ||||||| || ||||| || ||| |||
Sbjct 32580 GGTGCTACTGTAGAAAATACTTTATTTGAAGATGGTGATGGTGCAAATACATTCCGTGCT 32639
Query 10475 TTTGATAGCACTCAAGAAGAAGAGACCTATTCGATGGTTACTGCTAACCGTTTCTGGTCC 10534
||| | ||||||| ||||| || ||||| ||||| || |||||||| || |||||
Sbjct 32640 TTTAACCCAACTCAAGCCGAAGAAACTTATTCAATGGTCACCGCTAACCGCTTTTGGTCA 32699
Query 10535 CAGATATTTGGAATTGCTTTTAGCAATAAACGCTGGCTTCATTTCTTTATGCTGTTTGTA 10594
|| || ||||| |||||||| ||||||||| ||| | |||||||||||| | ||||||
Sbjct 32700 CAAATCTTTGGGGTTGCTTTTTCCAATAAACGTTGGTTACATTTCTTTATGTTATTTGTA 32759
Query 10595 CCTGTTATGGGTCTTTGGACCTCTTCTATTGGAATCATTGGTCTTGCTCTCAATCTTCGT 10654
|| || | ||| | |||| | || ||||| | | ||||| ||| | || || |||
Sbjct 32760 CCAGTAACTGGTTTATGGATGAGTGCTCTTGGAGTAGTCGGTCTAGCTTTGAACCTACGT 32819
Query 10655 GCTTACGATTTTGTATCTCAAGAGATCAGGGCGTCAGAAGATCCTGAATTTGAGACGTTC 10714
|| || || || || || || || ||| | || | |||||||| ||||||||||| |||
Sbjct 32820 GCCTATGACTTCGTTTCCCAGGAAATCCGTGCAGCGGAAGATCCGGAATTTGAGACTTTC 32879
Query 10715 TATACGAAGAATATCCTTCTGAACGAAGGTCTTCGTGCATGGATGGCACCAGTTGACCAA 10774
||||| || ||||| ||| | ||||||||| |||| || |||||||| | || ||
Sbjct 32880 TATACTAAAAATATTCTTTTAAACGAAGGTATTCGCGCTTGGATGGCGGCTCAAGATCAG 32939
Query 10775 CCACACGAAAGCTTTGTTTTCCCAGAAGAAGTTCTTCC 10812
|| || |||| | || | ||||| || || ||||| ||
Sbjct 32940 CCTCATGAAAACCTTATATTCCCTGAGGAGGTTCTACC 32977
>seq3905
Length=4339
Score = 286 bits (316), Expect = 4e-72
Identities = 311/413 (75%), Gaps = 0/413 (0%)
Strand=Plus/Plus
Query 10100 CTCTGGGCTTTTGTGGCGCTCCACGGAGCCTTCGCTTTAATCGGCTTTATGTTACGCCAG 10159
|| ||||||||||| || |||||||| || ||||| ||||| || ||||||||||| ||
Sbjct 1156 CTGTGGGCTTTTGTTGCTCTCCACGGTGCTTTCGCATTAATAGGTTTTATGTTACGTCAA 1215
Query 10160 TTTGAGCTGGCGAGATTAATTGGAATCCGACCTTACAATGCTATCGCGTTTTCTGGGCCT 10219
||||| || || ||| || | | |||||||| ||||| ||||| || | ||| ||
Sbjct 1216 TTTGAACTTGCTCGATCTGTTCAATTGCGACCTTATAATGCAATCGCATTCTTTGGTCCA 1275
Query 10220 ATTGCTGTTTTTGTCAGTGTTTTCCTCATCTATCCACTCGGACAGTCTAGTTGGTTCTTT 10279
|||||||||||||| ||| || || || |||||||| || || ||| |||||||||||
Sbjct 1276 ATTGCTGTTTTTGTTTCTGTCTTTCTAATTTATCCACTAGGTCAATCTGGTTGGTTCTTT 1335
Query 10280 GCGCCGAGTTTCGGCGTCGCGGCGATTTTCCGCTTCCTTCTCTTCCTCCAGGGCTTTCAT 10339
||||| ||||| || || |||| || || || ||| | ||||| |||| || ||||||
Sbjct 1336 GCGCCTAGTTTTGGTGTAGCGGTTATATTTCGATTCATCCTCTTTTTCCAAGGGTTTCAT 1395
Query 10340 AATTGGACGCTCAATCCCTTCCATATGATGGGAGTTGCTGGTATACTGGGAGGGGCGCTC 10399
||||||| | || || || |||||||||||||| |||||| ||||||| | ||| ||
Sbjct 1396 AATTGGATATTGAACCCATTTCATATGATGGGAGTCGCTGGTGTACTGGGCGCGGCTCTG 1455
Query 10400 CTATCAGCAATCCACGGAGTCACTGTAGAAAACACACTTTATCAAGATGGCGAACAAGCA 10459
||| || || || || | ||||||||||| || | | | ||||||| || |||
Sbjct 1456 TTATGCGCTATTCATGGTGCTACTGTAGAAAATACTTTATTTGAAGATGGTGATGGTGCA 1515
Query 10460 AACACTTTCAAAGCTTTTGATAGCACTCAAGAAGAAGAGACCTATTCGATGGT 10512
|| || ||| |||||| | ||||||| ||||| || ||||| |||||
Sbjct 1516 AATACATTCCGTGCTTTTAACCTAACTCAAGCCGAAGAAACTTATTCAATGGT 1568
>seq3
Length=29132933
Score = 224 bits (248), Expect = 1e-53
Identities = 368/528 (70%), Gaps = 2/528 (0%)
Strand=Plus/Plus
Query 9845 GATCGCTTTGTTTTTGTGGGTTGGTCTGGACTACTTCTTTTTCCCACTGCTTATCTTGCA 9904
|| |||||||||||||| ||||||||||| ||| | || ||||| ||| ||| | |
Sbjct 2931774 GACCGCTTTGTTTTTGTAGGTTGGTCTGGTCTATTGCTCTTTCCTTGTGCCTATTTCTCT 2931833
Query 9905 ATTGGTGGCTGGCTTACTGGCACTACGTTTGTCACGAGTTGGTACACTCACGGACTTGCA 9964
| || || ||| | || || || || ||||| || ||||| ||||| ||| | ||
Sbjct 2931834 TTGGGGGGTTGGTTCACAGGTACAACTTTTGTAACTTCATGGTATACTCATGGATTGGCT 2931893
Query 9965 AGTAGTTACCTTGAGGGTGCTAATTTCCTTACAGCGGCTGTCTCAACGCCTGCTGATGCT 10024
||| || | || ||| | | | | || | || || || || |||||| || |
Sbjct 2931894 AGTTCCTATTTAGAAGGTTGCAGTCTTTTAACCGATGCAGTTTCTACTCCTGCTAATAGT 2931953
Query 10025 ATGGGTCATTCTCTTCTTCTACTTTGGGGTCCTGAGTCTCAGGGAGATTTCGTCCGCTGG 10084
| |||||| || || | || ||||||||| | || |||||||| || |||
Sbjct 2931954 TTAACGCATTCT-TTGTTATTATTGTGGGTCCTGAAGCACAAGGAGATTTTAGTCGTTGG 2932012
Query 10085 ATCCAGCTTGGGGGACTCTGGGCTTTTGTGGCGCTCCACGGAGCCTTCGCTTTAATCGGC 10144
|| | | || || |||| |||||| || |||||| | || |||| ||||| |
Sbjct 2932013 TGTCAATTAGACGGTCTGTGGGGTTTTGTTGCTCTCCACAGTGCTTTCGAATTAATAAGT 2932072
Query 10145 TTTATGTTACGCCAGTTTGAGCTGGCGAGATTAATTGGAATCCGACCTTACAATGCTATC 10204
||||||||||| || ||||| || || ||| || | | |||||||| ||||| ||
Sbjct 2932073 TTTATGTTACGTCAATTTGAACTTGCTCGATCTGTTCAATTGCGACCTTATAATGCAATA 2932132
Query 10205 GCGTTTTCTGGGCCTATTGCTGTTTTTGTCAGTGTTTTCCTCATCTATCCACTCGGACAG 10264
| || ||||| || |||||||||||| | ||| || || || ||||| | || ||
Sbjct 2932133 ACATTCTCTGGTCCAATTGCTGTTTTTATTTCTGTCTTTCTAATTTATCCTC-AGGTCAA 2932191
Query 10265 TCTAGTTGGTTCTTTGCGCCGAGTTTCGGCGTCGCGGCGATTTTCCGCTTCCTTCTCTTC 10324
||| ||||||||||||| || ||||| || || ||||| || || || ||| | ||||
Sbjct 2932192 TCTGGTTGGTTCTTTGCACCTAGTTTTGGTGTAGCGGCTATATTTCGGTTCATCATCTTT 2932251
Query 10325 CTCCAGGGCTTTCATAATTGGACGCTCAATCCCTTCCATATGATGGGA 10372
|||| || ||||||||||||| | || || | ||||||||||||
Sbjct 2932252 TTCCAAGGGTTTCATAATTGGAAATTGAACCCTCTTCATATGATGGGA 2932299
>seq722
Length=22714
Score = 104 bits (114), Expect = 2e-17
Identities = 119/158 (75%), Gaps = 3/158 (2%)
Strand=Plus/Minus
Query 10217 CCTATTGCTGTTTTTGTCAGTGTTTTCCTCATCTATCCACTCGGACAGTCTAGTTGGTTC 10276
|| |||||||||||||| || ||| || || |||||||| | || | | ||||||||
Sbjct 7561 CCAATTGCTGTTTTTGTTTGTCTTT--CTAATTTATCCACTATGTCAATGTGGTTGGTTC 7504
Query 10277 TTTGCGCCGAGTTTCGGCGTCGCGGCGATTTTCCGCTTCCTTCTCTTCCTCCAGGGCTTT 10336
||| |||| ||||| | || | ||| || || || ||| ||||||| || | || |||
Sbjct 7503 TTTACGCCTAGTTTTGATGTAGTGGCTATATTTCGATTCATTCTCTTTTTCAAAGG-TTT 7445
Query 10337 CATAATTGGACGCTCAATCCCTTCCATATGATGGGAGT 10374
||||||||||| | || | | ||||||||||||||
Sbjct 7444 CATAATTGGACATTGAAACTAATTCATATGATGGGAGT 7407
>seq3442
Length=4740
Score = 96.9 bits (106), Expect = 4e-15
Identities = 73/86 (85%), Gaps = 0/86 (0%)
Strand=Plus/Plus
Query 8918 GTCAAGAAGAGGAAACTTACAACATTGTTGCCGCTCATGGCTACTTTGGTCGCTTGATCT 8977
|||||||| | ||||||||||||||||| || ||||| || || ||||| || |||||||
Sbjct 4277 GTCAAGAAAAAGAAACTTACAACATTGTAGCTGCTCACGGTTATTTTGGCCGATTGATCT 4336
Query 8978 TCCAATATGCATCTTTTAACAATTCT 9003
|||||||||| ||| |||||||||
Sbjct 4337 TCCAATATGCTAGTTTCAACAATTCT 4362
>seq68
Length=187690
Score = 91.5 bits (100), Expect = 2e-13
Identities = 94/122 (77%), Gaps = 1/122 (1%)
Strand=Plus/Plus
Query 8599 TCTTACCGCCTTGGCATGCGTCCTTGGATCTGTGTTGCATACTCTGCTCCTGTTGCCG-C 8657
|||| ||| || || ||||||| |||||| ||||||| | || ||||||||||| | |
Sbjct 91317 TCTTTCCGTCTGGGTATGCGTCTTTGGATTGTTGTTGCAGATTCAGCTCCTGTTGCAGCC 91376
Query 8658 TGCTTCTGCTGTATTCCTTGTCTATCCTTTTGGTCAAGGTTCGTTCTCTGATGGTATGCC 8717
|||| ||||||| ||| | |||| || ||||| | || || ||||||||||||||
Sbjct 91377 TGCTACTGCTGTTTTCTTGATCTACCCAATTGGTGATGGAAGTTTTTCTGATGGTATGCC 91436
Query 8718 TC 8719
||
Sbjct 91437 TC 91438
>seq7
Length=27187321
Score = 84.2 bits (92), Expect = 2e-11
Identities = 118/162 (73%), Gaps = 4/162 (2%)
Strand=Plus/Plus
Query 10211 TCTGGGCCTATTGCTGTTTTTGTCAGTGTTTTCCTCATCTATCCACTCGGACAGTCTAGT 10270
|||| || |||||||||||||| || ||| | | |||||||| | || | | ||
Sbjct 26671815 TCTGATCCAATTGCTGTTTTTGTTTGTCTTTCCA--AGTTATCCACTATGTCAATGTGGT 26671872
Query 10271 TGGTTCTTTGCGCCGAGTTTCGGCGTCGCGGCGATTTTCCGCTTCCTTCTCTTCCTCCAG 10330
|||||||||||||| ||||| || || ||| || || || ||| ||||||| || |
Sbjct 26671873 TGGTTCTTTGCGCCTAGTTT-GGGGTAATGGCTATATTTCGATTCATTCTCTTTTTCAAA 26671931
Query 10331 GGCTTTCATAATTGGACGCTCAATCCCTTCCATATGATGGGA 10372
| |||||||||||||| | || | | |||||||||||
Sbjct 26671932 AG-TTTCATAATTGGACATTGAACCTAATTTATATGATGGGA 26671972
Lambda K H
0.634 0.408 0.912
Gapped
Lambda K H
0.625 0.410 0.780
Effective search space used: 516411075744000
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