Alignment viewer
BLASTN 2.6.0+
Reference: Stephen F. Altschul, Thomas L. Madden, Alejandro A.
Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J.
Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of
protein database search programs", Nucleic Acids Res. 25:3389-3402.
Database: Eichhornia_20paniculata_20[genbank_20GCA_5F001647135.1_202016-05-02]
40,286 sequences; 571,388,321 total letters
Query= NC_023442.1
Length=120420
Score E
Sequences producing significant alignments: (Bits) Value
seq490 149 5e-31
seq5010 131 1e-25
seq2385 127 2e-24
seq263 127 2e-24
seq220 122 7e-23
>seq490
Length=81643
Score = 149 bits (164), Expect = 5e-31
Identities = 157/204 (77%), Gaps = 2/204 (1%)
Strand=Plus/Minus
Query 29584 TGAAGCGTGGATTCTTTTTGAATGTTATAATCCGCAATGGTGCGAGAATCATCGAGTTGT 29643
|| || || ||||| || || ||||| ||||| |||| |||||| ||| ||||| ||
Sbjct 59260 TGGAGTGTAGATTCCTTCTGGATGTTGTAATCAGCAAGGGTGCGTCCATCTTCGAGCTGC 59201
Query 29644 TTGCCAGCATATATCAATCTTTGCTGATCCGGCGGAATACCCTCTTTGTCTTGTAATTTT 29703
|| || ||| |||| | ||||||||| ||||| ||||| ||||| ||||| || ||| ||
Sbjct 59200 TTCCCTGCAAATATGAGTCTTTGCTGGTCCGGGGGAATGCCCTCCTTGTCCTG-AATCTT 59142
Query 29704 TGT-TTGACAGTTTCTACAGTGTCAGAAAGTTCTACTTCAAGAGTTATAGTTTTGCCAGT 29762
|| ||||| | || | ||||| || ||| || ||||| || ||||| || |||||
Sbjct 59141 TGCCTTGACGTTGTCAATGGTGTCTGACGATTCAACCTCAAGGGTAATAGTCTTACCAGT 59082
Query 29763 TAAGGTTTTAACAAATATCTGCAT 29786
||||| |||||||||||||||||
Sbjct 59081 CAAGGTCTTAACAAATATCTGCAT 59058
Score = 131 bits (144), Expect = 1e-25
Identities = 158/215 (73%), Gaps = 0/215 (0%)
Strand=Plus/Minus
Query 29572 CGCAGCACCAAATGAAGCGTGGATTCTTTTTGAATGTTATAATCCGCAATGGTGCGAGAA 29631
|| ||||| | || |||||||| || ||||||||||| || || || | ||||||| |
Sbjct 58816 CGAAGCACAAGGTGGAGCGTGGACTCCTTTTGAATGTTGTAGTCAGCCAGGGTGCGACCA 58757
Query 29632 TCATCGAGTTGTTTGCCAGCATATATCAATCTTTGCTGATCCGGCGGAATACCCTCTTTG 29691
|| || | ||| ||||| ||| | |||| | ||||| ||||| || || ||||| |||
Sbjct 58756 TCCTCCAATTGCTTGCCTGCAAAGATCAGACGCTGCTGGTCCGGGGGGATTCCCTCCTTG 58697
Query 29692 TCTTGTAATTTTTGTTTGACAGTTTCTACAGTGTCAGAAAGTTCTACTTCAAGAGTTATA 29751
|| || | || |||||| | || | ||| || ||| || || |||||||| ||
Sbjct 58696 TCCTGGATCTTGGCCTTGACATTGTCAATAGTATCTGAAGACTCAACCTCAAGAGTGATT 58637
Query 29752 GTTTTGCCAGTTAAGGTTTTAACAAATATCTGCAT 29786
|| ||||| || ||||| || ||||| ||||||||
Sbjct 58636 GTCTTGCCTGTCAAGGTCTTCACAAAGATCTGCAT 58602
Score = 125 bits (138), Expect = 6e-24
Identities = 159/216 (74%), Gaps = 2/216 (1%)
Strand=Plus/Minus
Query 29572 CGCAGCACCAAATGAAGCGTGGATTCTTTTTGAATGTTATAATCCGCAATGGTGCGAGAA 29631
|| || |||| ||| || || || || |||||||| || || || || | |||||| |
Sbjct 59728 CGAAGAACCAGATGCAGGGTTGACTCCTTTTGAATATTGTAGTCTGCTAGGGTGCGCCCA 59669
Query 29632 TCATCGAGTTGTTTGCCAGCATATATCAATCTTTGCTGATCCGGCGGAATACCCTCTTTG 29691
|| || || || || || ||| | || | || || || || || ||||| |||||||||
Sbjct 59668 TCCTCAAGCTGCTTCCCCGCAAAGATGAGCCTCTGTTGGTCTGGAGGAATGCCCTCTTTG 59609
Query 29692 TCTTGTAATTTTTGT-TTGACAGTTTCTACAGTGTCAGAAAGTTCTACTTCAAGAGTTAT 29750
|| || |||||||| |||||| | || | ||||| ||| ||| || ||||| |||||
Sbjct 59608 TCCTG-AATTTTTGCCTTGACATTGTCAATGGTGTCTGAAGATTCAACCTCAAGGGTTAT 59550
Query 29751 AGTTTTGCCAGTTAAGGTTTTAACAAATATCTGCAT 29786
|| |||||||| | || || || |||||||||||
Sbjct 59549 GGTCTTGCCAGTCAGAGTCTTGACGAATATCTGCAT 59514
Score = 120 bits (132), Expect = 2e-22
Identities = 156/216 (72%), Gaps = 0/216 (0%)
Strand=Plus/Minus
Query 29571 ACGCAGCACCAAATGAAGCGTGGATTCTTTTTGAATGTTATAATCCGCAATGGTGCGAGA 29630
||| || || | || ||||| ||||| ||||||||||||||||| |||| || ||
Sbjct 59045 ACGGAGGACAAGGTGGAGCGTAGATTCCTTTTGAATGTTATAATCAGCAAGAGTACGTCC 58986
Query 29631 ATCATCGAGTTGTTTGCCAGCATATATCAATCTTTGCTGATCCGGCGGAATACCCTCTTT 29690
||| || || || || || ||| | || | |||||||| || || || || ||||||||
Sbjct 58985 ATCCTCAAGCTGCTTCCCTGCAAAGATGAGCCTTTGCTGGTCAGGGGGGATGCCCTCTTT 58926
Query 29691 GTCTTGTAATTTTTGTTTGACAGTTTCTACAGTGTCAGAAAGTTCTACTTCAAGAGTTAT 29750
|| || | || ||||||| | || | ||||| || || || ||||| || ||
Sbjct 58925 ATCCTGGATCTTCGCTTTGACATTGTCAATGGTGTCCGAGGACTCAACCTCAAGGGTGAT 58866
Query 29751 AGTTTTGCCAGTTAAGGTTTTAACAAATATCTGCAT 29786
||| ||||| || || || || ||||| ||||||||
Sbjct 58865 AGTCTTGCCCGTCAATGTCTTCACAAAGATCTGCAT 58830
Score = 113 bits (124), Expect = 3e-20
Identities = 149/204 (73%), Gaps = 2/204 (1%)
Strand=Plus/Minus
Query 29584 TGAAGCGTGGATTCTTTTTGAATGTTATAATCCGCAATGGTGCGAGAATCATCGAGTTGT 29643
|| || || || || || || ||||| || || || | ||| || ||| ||||| |||
Sbjct 58576 TGGAGTGTTGACTCCTTCTGGATGTTGTAGTCAGCCAGGGTACGGCCATCCTCGAGCTGT 58517
Query 29644 TTGCCAGCATATATCAATCTTTGCTGATCCGGCGGAATACCCTCTTTGTCTTGTAATTTT 29703
||||||||| | |||| | |||||| ||||| || |||||||| ||||| || ||||||
Sbjct 58516 TTGCCAGCAAAGATCAGACGTTGCTGGTCCGGAGGGATACCCTCCTTGTCCTG-AATTTT 58458
Query 29704 TG-TTTGACAGTTTCTACAGTGTCAGAAAGTTCTACTTCAAGAGTTATAGTTTTGCCAGT 29762
| |||||| | || | || ||||| || || |||||||| || || ||||| ||
Sbjct 58457 GGCCTTGACATTATCAATTGTATCAGATGACTCGACCTCAAGAGTGATGGTCTTGCCTGT 58398
Query 29763 TAAGGTTTTAACAAATATCTGCAT 29786
|| || || || || || |||||
Sbjct 58397 CAATGTCTTGACGAAGATTTGCAT 58374
Score = 111 bits (122), Expect = 1e-19
Identities = 154/216 (71%), Gaps = 0/216 (0%)
Strand=Plus/Minus
Query 29571 ACGCAGCACCAAATGAAGCGTGGATTCTTTTTGAATGTTATAATCCGCAATGGTGCGAGA 29630
||| || || | || || || ||||| || || ||||||||||| |||| |||||
Sbjct 59501 ACGGAGGACAAGGTGGAGTGTAGATTCCTTCTGGATGTTATAATCAGCAAGTGTGCGTCC 59442
Query 29631 ATCATCGAGTTGTTTGCCAGCATATATCAATCTTTGCTGATCCGGCGGAATACCCTCTTT 29690
||| ||||| || || || ||| | || | |||||||| ||||| || |||||||| ||
Sbjct 59441 ATCCTCGAGCTGCTTCCCTGCAAAGATGAGCCTTTGCTGGTCCGGGGGGATACCCTCCTT 59382
Query 29691 GTCTTGTAATTTTTGTTTGACAGTTTCTACAGTGTCAGAAAGTTCTACTTCAAGAGTTAT 29750
||| || | || || ||| | || | || || || ||| || ||||| || ||
Sbjct 59381 GTCCTGGATCTTGGCCTTAACATTGTCGATGGTATCCGATGATTCAACCTCAAGGGTGAT 59322
Query 29751 AGTTTTGCCAGTTAAGGTTTTAACAAATATCTGCAT 29786
|| |||||||| | || || ||||| ||||||||
Sbjct 59321 GGTCTTGCCAGTCAGTGTCTTCACAAAGATCTGCAT 59286
>seq5010
Length=51657
Score = 131 bits (144), Expect = 1e-25
Identities = 158/215 (73%), Gaps = 0/215 (0%)
Strand=Plus/Minus
Query 29572 CGCAGCACCAAATGAAGCGTGGATTCTTTTTGAATGTTATAATCCGCAATGGTGCGAGAA 29631
|| ||||||| || || ||||||||||| |||||||||||||| |||| ||| || |
Sbjct 38734 CGGAGCACCAGGTGGAGGGTGGATTCTTTCTGAATGTTATAATCGGCAAGGGTACGGCCA 38675
Query 29632 TCATCGAGTTGTTTGCCAGCATATATCAATCTTTGCTGATCCGGCGGAATACCCTCTTTG 29691
|| || || || |||||||| | || | || ||||||||||| ||||| ||||| |||
Sbjct 38674 TCCTCAAGCTGCTTGCCAGCGAAGATGAGCCTCTGCTGATCCGGGGGAATCCCCTCCTTG 38615
Query 29692 TCTTGTAATTTTTGTTTGACAGTTTCTACAGTGTCAGAAAGTTCTACTTCAAGAGTTATA 29751
|| || | ||| || || | || | || || || || |||||||| || ||
Sbjct 38614 TCCTGGATTTTAGCCTTAACGTTGTCAATGGTATCCGAGCTCTCGACTTCAAGGGTGATG 38555
Query 29752 GTTTTGCCAGTTAAGGTTTTAACAAATATCTGCAT 29786
||||||||||| | || || || || ||||||||
Sbjct 38554 GTTTTGCCAGTAAGTGTCTTGACGAAGATCTGCAT 38520
Score = 120 bits (132), Expect = 2e-22
Identities = 156/216 (72%), Gaps = 0/216 (0%)
Strand=Plus/Minus
Query 29571 ACGCAGCACCAAATGAAGCGTGGATTCTTTTTGAATGTTATAATCCGCAATGGTGCGAGA 29630
||| || |||| || || ||||| || || || |||||||| || || | ||||||
Sbjct 38051 ACGGAGGACCAGGTGGAGGGTGGACTCCTTCTGGATGTTATAGTCGGCGAGGGTGCGGCC 37992
Query 29631 ATCATCGAGTTGTTTGCCAGCATATATCAATCTTTGCTGATCCGGCGGAATACCCTCTTT 29690
||| ||||||||||| || ||| | ||||| || ||||||||||| || || ||||| ||
Sbjct 37991 ATCCTCGAGTTGTTTACCGGCAAAGATCAACCTCTGCTGATCCGGGGGGATGCCCTCCTT 37932
Query 29691 GTCTTGTAATTTTTGTTTGACAGTTTCTACAGTGTCAGAAAGTTCTACTTCAAGAGTTAT 29750
||| || | || ||| || | || | ||||| ||| ||| || || || || ||
Sbjct 37931 GTCCTGGATCTTGGCTTTAACGTTGTCGATGGTGTCGGAAGATTCCACCTCGAGGGTGAT 37872
Query 29751 AGTTTTGCCAGTTAAGGTTTTAACAAATATCTGCAT 29786
|| || || || | ||| || || || ||||||||
Sbjct 37871 GGTCTTTCCGGTGAGGGTCTTGACGAAGATCTGCAT 37836
>seq2385
Length=44452
Score = 127 bits (140), Expect = 2e-24
Identities = 160/217 (74%), Gaps = 2/217 (1%)
Strand=Plus/Minus
Query 29572 CGCAGCACCAAATGAAGCGTGGATTCTTTTTGAATGTTATAATCCGCAATGGTGCGAGAA 29631
|| || |||| ||| || || || || |||||||| || || || || | ||||||
Sbjct 35092 CGAAGAACCAGATGCAGGGTCGACTCCTTTTGAATATTGTAGTCTGCTAGGGTGCGCCCG 35033
Query 29632 TCATCGAGTTGTTTGCCAGCATATATCAATCTTTGCTGATCCGGCGGAATACCCTCTTTG 29691
|| || || || || |||||| | || | || ||||| ||||| ||||| |||||||||
Sbjct 35032 TCCTCAAGCTGCTTCCCAGCAAAGATGAGCCTCTGCTGGTCCGGAGGAATGCCCTCTTTG 34973
Query 29692 TCTTGTAATTTTTG-TTTGACAGTTTCTACAGTGTCAGAAAGTTCTACTTCAAGAGTTAT 29750
|| || ||| |||| ||||| | || | ||||| ||| ||| || ||||| || ||
Sbjct 34972 TCCTG-AATCTTTGCCTTGACGTTGTCAATGGTGTCTGAAGATTCAACCTCAAGGGTAAT 34914
Query 29751 AGTTTTGCCAGTTAAGGTTTTAACAAATATCTGCATT 29787
|| |||||||| | ||| || ||||| |||||||||
Sbjct 34913 GGTCTTGCCAGTCAGGGTCTTGACAAAGATCTGCATT 34877
Score = 116 bits (128), Expect = 3e-21
Identities = 157/216 (73%), Gaps = 2/216 (1%)
Strand=Plus/Minus
Query 29572 CGCAGCACCAAATGAAGCGTGGATTCTTTTTGAATGTTATAATCCGCAATGGTGCGAGAA 29631
|| || || | ||||||||| ||||| || || ||||| ||||| || | ||| || |
Sbjct 34636 CGGAGGACGAGATGAAGCGTAGATTCCTTCTGGATGTTGTAATCAGCGAGGGTTCGCCCA 34577
Query 29632 TCATCGAGTTGTTTGCCAGCATATATCAATCTTTGCTGATCCGGCGGAATACCCTCTTTG 29691
|| || | ||||| || ||| | || | || |||||||| || ||||| ||||| ||
Sbjct 34576 TCTTCCAACTGTTTCCCTGCAAAGATAAGCCTCTGCTGATCTGGAGGAATGCCCTCCTTA 34517
Query 29692 TCTTGTAATTTTTGT-TTGACAGTTTCTACAGTGTCAGAAAGTTCTACTTCAAGAGTTAT 29750
|| || ||| |||| |||||| | || | ||||| || || || ||||| || ||
Sbjct 34516 TCCTG-AATCTTTGCCTTGACATTGTCGATGGTGTCCGATGACTCAACCTCAAGGGTGAT 34458
Query 29751 AGTTTTGCCAGTTAAGGTTTTAACAAATATCTGCAT 29786
||||||||||| | || || ||||| ||||||||
Sbjct 34457 CGTTTTGCCAGTCAGCGTCTTCACAAAGATCTGCAT 34422
Score = 111 bits (122), Expect = 1e-19
Identities = 163/228 (71%), Gaps = 2/228 (1%)
Strand=Plus/Minus
Query 29560 CCTCCCCTCAAACGCAGCACCAAATGAAGCGTGGATTCTTTTTGAATGTTATAATCCGCA 29619
||||| | | ||| || || | || || || || || || || ||||| || || ||
Sbjct 34041 CCTCCACGAAGACGGAGAACAAGGTGGAGTGTTGACTCCTTCTGGATGTTGTAGTCAGCC 33982
Query 29620 ATGGTGCGAGAATCATCGAGTTGTTTGCCAGCATATATCAATCTTTGCTGATCCGGCGGA 29679
| |||||| ||| |||||||||||||||||| | |||| | ||||| ||||| |||
Sbjct 33981 AGGGTGCGGCCATCCTCGAGTTGTTTGCCAGCAAAGATCAGACGCTGCTGGTCCGGAGGA 33922
Query 29680 ATACCCTCTTTGTCTTGTAATTTTTG-TTTGACAGTTTCTACAGTGTCAGAAAGTTCTAC 29738
|| ||||| ||||| || |||||| | || ||| | || | || || || || ||
Sbjct 33921 ATTCCCTCCTTGTCCTG-AATTTTGGCCTTAACATTGTCGATTGTATCGGATGACTCGAC 33863
Query 29739 TTCAAGAGTTATAGTTTTGCCAGTTAAGGTTTTAACAAATATCTGCAT 29786
|||||||| || || || || || || || || ||||| || |||||
Sbjct 33862 CTCAAGAGTGATGGTCTTCCCGGTCAATGTCTTTACAAAGATTTGCAT 33815
Score = 109 bits (120), Expect = 4e-19
Identities = 146/203 (72%), Gaps = 0/203 (0%)
Strand=Plus/Minus
Query 29584 TGAAGCGTGGATTCTTTTTGAATGTTATAATCCGCAATGGTGCGAGAATCATCGAGTTGT 29643
|| ||||| ||||| ||||| ||||| ||||| || | ||||| ||| || || ||
Sbjct 34852 TGGAGCGTAGATTCCTTTTGGATGTTGTAATCAGCCAAAGTGCGTCCATCCTCAAGCTGC 34793
Query 29644 TTGCCAGCATATATCAATCTTTGCTGATCCGGCGGAATACCCTCTTTGTCTTGTAATTTT 29703
|| || ||| | || | |||||||| ||||| || || ||||| |||||||| | ||
Sbjct 34792 TTCCCTGCAAAAATGAGCCTTTGCTGGTCCGGGGGGATGCCCTCCTTGTCTTGGATCTTG 34733
Query 29704 TGTTTGACAGTTTCTACAGTGTCAGAAAGTTCTACTTCAAGAGTTATAGTTTTGCCAGTT 29763
|| ||| | || | ||||| || || || ||||| || || |||||||||||
Sbjct 34732 GCCTTAACATTGTCGATGGTGTCCGATGACTCAACCTCAAGGGTGATCGTTTTGCCAGTC 34673
Query 29764 AAGGTTTTAACAAATATCTGCAT 29786
| || || ||||| ||||||||
Sbjct 34672 AGCGTCTTCACAAAGATCTGCAT 34650
>seq263
Length=97214
Score = 127 bits (140), Expect = 2e-24
Identities = 157/215 (73%), Gaps = 0/215 (0%)
Strand=Plus/Plus
Query 29572 CGCAGCACCAAATGAAGCGTGGATTCTTTTTGAATGTTATAATCCGCAATGGTGCGAGAA 29631
|| |||||||| || || |||||||| || ||||| || || || || | |||||| |
Sbjct 77863 CGGAGCACCAAGTGCAGGGTGGATTCCTTCTGAATATTGTAGTCGGCCAAGGTGCGGCCA 77922
Query 29632 TCATCGAGTTGTTTGCCAGCATATATCAATCTTTGCTGATCCGGCGGAATACCCTCTTTG 29691
|| || || || || ||||| | || | || ||||||||||| ||||||||||| |||
Sbjct 77923 TCCTCAAGCTGCTTTCCAGCGAAGATGAGCCTCTGCTGATCCGGGGGAATACCCTCCTTG 77982
Query 29692 TCTTGTAATTTTTGTTTGACAGTTTCTACAGTGTCAGAAAGTTCTACTTCAAGAGTTATA 29751
|| || | ||| ||| ||| | || | ||||| || || || ||||| |||||
Sbjct 77983 TCCTGGATTTTGGCTTTAACATTGTCAATGGTGTCCGAGCTCTCGACCTCAAGGGTTATG 78042
Query 29752 GTTTTGCCAGTTAAGGTTTTAACAAATATCTGCAT 29786
|| |||||||| | || || || || ||||||||
Sbjct 78043 GTCTTGCCAGTGAGCGTCTTGACGAAGATCTGCAT 78077
>seq220
Length=196394
Score = 122 bits (134), Expect = 7e-23
Identities = 157/217 (72%), Gaps = 0/217 (0%)
Strand=Plus/Plus
Query 29571 ACGCAGCACCAAATGAAGCGTGGATTCTTTTTGAATGTTATAATCCGCAATGGTGCGAGA 29630
||| ||||||| ||| || ||||| ||||| ||||||||||| || || | |||||
Sbjct 148783 ACGAAGCACCAGATGGAGAGTGGACTCTTTCTGAATGTTATAGTCTGCCAAAGTGCGCCC 148842
Query 29631 ATCATCGAGTTGTTTGCCAGCATATATCAATCTTTGCTGATCCGGCGGAATACCCTCTTT 29690
||| || || || || ||||| | || | ||| ||||| |||||||| || ||||| ||
Sbjct 148843 ATCCTCCAGCTGCTTCCCAGCGAAGATGAGTCTCTGCTGGTCCGGCGGGATCCCCTCCTT 148902
Query 29691 GTCTTGTAATTTTTGTTTGACAGTTTCTACAGTGTCAGAAAGTTCTACTTCAAGAGTTAT 29750
|||||| | || || ||| | || | ||||||||| || || || || || ||
Sbjct 148903 GTCTTGGATCTTCGCCTTCACATTATCAATAGTGTCAGAGCTCTCCACCTCCAGGGTAAT 148962
Query 29751 AGTTTTGCCAGTTAAGGTTTTAACAAATATCTGCATT 29787
|| || ||||| | ||| || || || || ||||||
Sbjct 148963 GGTCTTACCAGTGAGGGTCTTCACGAAGATTTGCATT 148999
Score = 122 bits (134), Expect = 7e-23
Identities = 166/229 (72%), Gaps = 2/229 (1%)
Strand=Plus/Plus
Query 29559 TCCTCCCCTCAAACGCAGCACCAAATGAAGCGTGGATTCTTTTTGAATGTTATAATCCGC 29618
||| ||||| | | ||||||||| || || || ||||| || |||||||| || || ||
Sbjct 148999 TCCACCCCTAAGCCTCAGCACCAAGTGGAGGGTAGATTCCTTCTGAATGTTGTAGTCAGC 149058
Query 29619 AATGGTGCGAGAATCATCGAGTTGTTTGCCAGCATATATCAATCTTTGCTGATCCGGCGG 29678
|| ||||| ||| ||||| || || ||||| | || | || ||||||||||| ||
Sbjct 149059 AAGAGTGCGCCCATCCTCGAGCTGCTTACCAGCGAAGATGAGCCTCTGCTGATCCGGGGG 149118
Query 29679 AATACCCTCTTTGTCTTGTAATTTTTG-TTTGACAGTTTCTACAGTGTCAGAAAGTTCTA 29737
||| || || ||||| || ||| || | || ||| | || | ||||| || || |
Sbjct 149119 AATGCCTTCCTTGTCCTG-AATCTTCGCCTTTACATTGTCGATGGTGTCTGAGCTCTCAA 149177
Query 29738 CTTCAAGAGTTATAGTTTTGCCAGTTAAGGTTTTAACAAATATCTGCAT 29786
| || || || || || |||||||| | ||| || ||||| ||||||||
Sbjct 149178 CCTCGAGGGTGATCGTCTTGCCAGTGAGGGTCTTGACAAAGATCTGCAT 149226
Lambda K H
0.634 0.408 0.912
Gapped
Lambda K H
0.625 0.410 0.780
Effective search space used: 385030062910326
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