Alignment viewer

BLASTN 2.6.0+ Reference: Stephen F. Altschul, Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Database: Tritrichomonas_20foetus_20[genbank_20GCA_5F001839685.1_202016-10-25] 1,480 sequences; 67,585,742 total letters Query= NC_021923.1 Length=140633 Score E Sequences producing significant alignments: (Bits) Value seq176 125 6e-24 seq827 111 1e-19 seq785 111 1e-19 seq548 107 2e-18 seq393 98.7 9e-16 seq1024 96.9 3e-15 seq1149 93.3 4e-14 seq959 93.3 4e-14 seq187 93.3 4e-14 seq716 89.7 5e-13 seq576 89.7 5e-13 seq788 86.0 6e-12 >seq176 Length=130081 Score = 125 bits (138), Expect = 6e-24 Identities = 160/215 (74%), Gaps = 8/215 (4%) Strand=Plus/Plus Query 24703 GATGCAGATCTTTATTAAAACGCTAACCGGTAAAACTATTACGCTCGAAGTAGAAGCCAC 24762 ||||||||||||| | || ||||| || || || ||||| || ||||| ||| | || Sbjct 85001 GATGCAGATCTTTGTCAAGACGCTGACAGGGAAGCACATTACACTGGAAGTTGAACCAAC 85060 Query 24763 CGACACAGTAGA-AACGGTAAAACAAAAGATCCAAGACAAAGAAGGAATACCTCCCGATC 24821 |||| | | || ||| || | ||| |||||||||||||||||||| || || |||| Sbjct 85061 AGACAGAATCGAGGACGTCAAGGCTAAA-ATCCAAGACAAAGAAGGAATCCCCCCAGATC 85119 Query 24822 AACAACGTTTGATCTTTGCCGGGAAACAGCTGGAGGATTCGAGAAACA---TGTCAGATT 24878 |||||||||||||||| ||||| ||||| |||| || || |||||| | ||| | Sbjct 85120 AACAACGTTTGATCTTCGCCGGAAAACAATTGGAAGA--CG-GAAACACCCTCCAAGACT 85176 Query 24879 ATAACATTCAAAAGGAATCGACACTACACTTGGTG 24913 | ||||||||||| || || || ||| | ||| Sbjct 85177 ACTCAATTCAAAAGGACTCAACTCTCCACCTTGTG 85211 >seq827 Length=184754 Score = 111 bits (122), Expect = 1e-19 Identities = 157/215 (73%), Gaps = 8/215 (4%) Strand=Plus/Plus Query 24703 GATGCAGATCTTTATTAAAACGCTAACCGGTAAAACTATTACGCTCGAAGTAGAAGCCAC 24762 ||||||||||||| | || ||||| || || || || || || ||||| ||| | || Sbjct 148853 GATGCAGATCTTTGTGAAGACGCTGACTGGGAAGCACATCACTCTGGAAGTTGAACCAAC 148912 Query 24763 CGACACAGTAGA-AACGGTAAAACAAAAGATCCAAGACAAAGAAGGAATACCTCCCGATC 24821 |||| | | || ||| || | ||| ||||||||||| |||||||| || || |||| Sbjct 148913 AGACAGAATCGAGGACGTCAAGGCCAAA-ATCCAAGACAAGGAAGGAATTCCACCAGATC 148971 Query 24822 AACAACGTTTGATCTTTGCCGGGAAACAGCTGGAGGATTCGAGAAACA---TGTCAGATT 24878 |||||||||||||||| ||||| ||||| |||| || || |||||| | ||||| Sbjct 148972 AACAACGTTTGATCTTCGCCGGAAAACAATTGGAAGA--CG-GAAACACCCTCCAAGATT 149028 Query 24879 ATAACATTCAAAAGGAATCGACACTACACTTGGTG 24913 | || ||||| || || || || ||| | ||| Sbjct 149029 ACTCAATCCAAAAAGATTCAACTCTCCACCTTGTG 149063 >seq785 Length=62999 Score = 111 bits (122), Expect = 1e-19 Identities = 121/158 (77%), Gaps = 2/158 (1%) Strand=Plus/Plus Query 24703 GATGCAGATCTTTATTAAAACGCTAACCGGTAAAACTATTACGCTCGAAGTAGAAGCCAC 24762 ||||||||||||| | || || || || || || ||||| || ||||| ||| | || Sbjct 32410 GATGCAGATCTTTGTGAAGACACTGACAGGGAAGCACATTACTCTGGAAGTTGAACCAAC 32469 Query 24763 CGACACAGTAGA-AACGGTAAAACAAAAGATCCAAGACAAAGAAGGAATACCTCCCGATC 24821 |||| | | || ||| || | ||| |||||||||||||||||||| || || |||| Sbjct 32470 AGACAGAATCGAGGACGTCAAGGCCAAA-ATCCAAGACAAAGAAGGAATTCCACCAGATC 32528 Query 24822 AACAACGTTTGATCTTTGCCGGGAAACAGCTGGAGGAT 24859 ||||||||||||| || ||||| ||||| |||| ||| Sbjct 32529 AACAACGTTTGATTTTCGCCGGAAAACAATTGGAAGAT 32566 >seq548 Length=109954 Score = 107 bits (118), Expect = 2e-18 Identities = 156/215 (73%), Gaps = 8/215 (4%) Strand=Plus/Minus Query 24703 GATGCAGATCTTTATTAAAACGCTAACCGGTAAAACTATTACGCTCGAAGTAGAAGCCAC 24762 |||||||||||| | || ||||| || || || ||||| || ||||| ||| | || Sbjct 39932 GATGCAGATCTTCGTAAAGACGCTGACAGGGAAGCACATTACTCTGGAAGTTGAACCAAC 39873 Query 24763 CGACACAGTAGAA-ACGGTAAAACAAAAGATCCAAGACAAAGAAGGAATACCTCCCGATC 24821 |||| | || ||| || | ||| ||||||||||| |||||||| || || |||| Sbjct 39872 AGACAGCATCGAGGACGTCAAGGCCAAA-ATCCAAGACAAGGAAGGAATTCCACCAGATC 39814 Query 24822 AACAACGTTTGATCTTTGCCGGGAAACAGCTGGAGGATTCGAGAAACA---TGTCAGATT 24878 |||||||||||||||| || || ||||| |||| || || | |||| | ||||| Sbjct 39813 AACAACGTTTGATCTTCGCTGGAAAACAATTGGAAGA--CG-GCAACACCCTCCAAGATT 39757 Query 24879 ATAACATTCAAAAGGAATCGACACTACACTTGGTG 24913 | ||||||||||| || || || ||| | ||| Sbjct 39756 ACTCAATTCAAAAGGATTCAACTCTCCACCTTGTG 39722 >seq393 Length=120711 Score = 98.7 bits (108), Expect = 9e-16 Identities = 150/208 (72%), Gaps = 8/208 (4%) Strand=Plus/Plus Query 24704 ATGCAGATCTTTATTAAAACGCTAACCGGTAAAACTATTACGCTCGAAGTAGAAGCCACC 24763 ||||| ||||| | || || || || || || |||||| |||||||| || | ||| Sbjct 67383 ATGCAAATCTTCGTCAAGACACTTACAGGAAAGCATATTACACTCGAAGTCGAGCCAACC 67442 Query 24764 GACACAGTAGAA-ACGGTAAAACAAAAGATCCAAGACAAAGAAGGAATACCTCCCGATCA 24822 || | ||| ||| |||| | || ||| ||||||||||||||||| || || ||||| Sbjct 67443 GATCGTATCGAAGACGTTAAAGCTAA-GATTCAAGACAAAGAAGGAATTCCACCAGATCA 67501 Query 24823 ACAACGTTTGATCTTTGCCGGGAAACAGCTGGAGGATTCGAGAAACA---TGTCAGATTA 24879 ||||||| | ||||| ||||| || || | || || || |||||| | |||||| Sbjct 67502 ACAACGTCTCATCTTCGCCGGTAAGCAATTAGAAGA--CG-GAAACACACTCCAAGATTA 67558 Query 24880 TAACATTCAAAAGGAATCGACACTACAC 24907 ||||||||||| || || || ||| Sbjct 67559 CTCAATTCAAAAGGACTCAACCCTTCAC 67586 >seq1024 Length=37034 Score = 96.9 bits (106), Expect = 3e-15 Identities = 151/213 (71%), Gaps = 6/213 (3%) Strand=Plus/Minus Query 24703 GATGCAGATCTTTATTAAAACGCTAACCGGTAAAACTATTACGCTCGAAGTAGAAGCCAC 24762 |||||| ||||| | ||||| || || || ||| || || |||||||| ||| | || Sbjct 17616 GATGCAAATCTTCGTCAAAACCCTCACAGGCAAACACATCACCCTCGAAGTCGAACCAAC 17557 Query 24763 CGACACAGTAGAAACGGTAAAACAAAAGATCCAAGACAAAGAAGGAATACCTCCCGATCA 24822 ||| | || || ||| |||||||||||||||||||| || || || ||||| Sbjct 17556 AGACCGTATCGAGGACGTCAAAGCCAAGATCCAAGACAAAGAAGGTATCCCACCAGATCA 17497 Query 24823 ACAACGTTTGATCTTTGCCGGGAAACAGCTGGAGGATTCGAGAAACA---TGTCAGATTA 24879 ||||||| | ||||| || || ||||| | || || || | |||| | ||| || Sbjct 17496 ACAACGTCTTATCTTCGCTGGTAAACAATTAGAAGA--CG-GCAACACCCTCCAAGACTA 17440 Query 24880 TAACATTCAAAAGGAATCGACACTACACTTGGT 24912 ||| |||||||| || || || |||||||| Sbjct 17439 CTCCATCCAAAAGGACTCAACCCTCCACTTGGT 17407 Score = 95.1 bits (104), Expect = 1e-14 Identities = 150/212 (71%), Gaps = 6/212 (3%) Strand=Plus/Minus Query 24704 ATGCAGATCTTTATTAAAACGCTAACCGGTAAAACTATTACGCTCGAAGTAGAAGCCACC 24763 ||||| ||||| | ||||| || || || || || || |||||||| ||| | || Sbjct 16931 ATGCAAATCTTCGTCAAAACCCTCACAGGAAAGCACATCACCCTCGAAGTCGAACCAACA 16872 Query 24764 GACACAGTAGAAACGGTAAAACAAAAGATCCAAGACAAAGAAGGAATACCTCCCGATCAA 24823 ||| | ||| || ||| |||||||||||||||||||| || || || |||||| Sbjct 16871 GACCGTATCGAAGATGTCAAAGCCAAGATCCAAGACAAAGAAGGTATCCCACCAGATCAA 16812 Query 24824 CAACGTTTGATCTTTGCCGGGAAACAGCTGGAGGATTCGAGAAACA---TGTCAGATTAT 24880 |||||| | || ||||| || ||||| | || || || | |||| | ||| || Sbjct 16811 CAACGTCTCATTTTTGCTGGTAAACAATTAGAAGA--CG-GCAACACCCTCCAAGACTAC 16755 Query 24881 AACATTCAAAAGGAATCGACACTACACTTGGT 24912 ||| |||||||| || || || |||||||| Sbjct 16754 TCCATCCAAAAGGATTCAACCCTCCACTTGGT 16723 Score = 86.0 bits (94), Expect = 6e-12 Identities = 147/212 (69%), Gaps = 6/212 (3%) Strand=Plus/Minus Query 24704 ATGCAGATCTTTATTAAAACGCTAACCGGTAAAACTATTACGCTCGAAGTAGAAGCCACC 24763 ||||| ||||| |||| || || || || || || || || ||||| ||| | || Sbjct 17159 ATGCAAATCTTCGTTAAGACACTCACAGGAAAGCACATCACCCTTGAAGTCGAACCAACA 17100 Query 24764 GACACAGTAGAAACGGTAAAACAAAAGATCCAAGACAAAGAAGGAATACCTCCCGATCAA 24823 || | ||| || || || |||||||||||||||||||| || || |||||| Sbjct 17099 GATCGTATCGAAGATGTCAAGGCTAAAATCCAAGACAAAGAAGGAATTCCACCAGATCAA 17040 Query 24824 CAACGTTTGATCTTTGCCGGGAAACAGCTGGAGGATTCGAGAAACA---TGTCAGATTAT 24880 |||||| | || ||||| || ||||| | || ||| |||||| | ||| || Sbjct 17039 CAACGTCTCATTTTTGCTGGTAAACAATTAGAAGAT---GGAAACACCCTCCAAGACTAC 16983 Query 24881 AACATTCAAAAGGAATCGACACTACACTTGGT 24912 ||| |||||||| || || || |||||||| Sbjct 16982 TCCATCCAAAAGGACTCAACCCTCCACTTGGT 16951 Score = 86.0 bits (94), Expect = 6e-12 Identities = 147/212 (69%), Gaps = 6/212 (3%) Strand=Plus/Minus Query 24704 ATGCAGATCTTTATTAAAACGCTAACCGGTAAAACTATTACGCTCGAAGTAGAAGCCACC 24763 ||||| ||||| |||| || || || || || || || || ||||| ||| | || Sbjct 17387 ATGCAAATCTTCGTTAAGACACTCACAGGAAAGCACATCACCCTTGAAGTCGAACCAACA 17328 Query 24764 GACACAGTAGAAACGGTAAAACAAAAGATCCAAGACAAAGAAGGAATACCTCCCGATCAA 24823 || | ||| || || || |||||||||||||||||||| || || |||||| Sbjct 17327 GATCGTATCGAAGATGTCAAGGCTAAAATCCAAGACAAAGAAGGAATTCCACCAGATCAA 17268 Query 24824 CAACGTTTGATCTTTGCCGGGAAACAGCTGGAGGATTCGAGAAACA---TGTCAGATTAT 24880 |||||| | || ||||| || ||||| | || ||| |||||| | ||| || Sbjct 17267 CAACGTCTCATTTTTGCTGGTAAACAATTAGAAGAT---GGAAACACCCTCCAAGACTAC 17211 Query 24881 AACATTCAAAAGGAATCGACACTACACTTGGT 24912 ||| |||||||| || || || |||||||| Sbjct 17210 TCCATCCAAAAGGACTCAACCCTCCACTTGGT 17179 >seq1149 Length=154106 Score = 93.3 bits (102), Expect = 4e-14 Identities = 148/210 (70%), Gaps = 2/210 (1%) Strand=Plus/Plus Query 24704 ATGCAGATCTTTATTAAAACGCTAACCGGTAAAACTATTACGCTCGAAGTAGAAGCCACC 24763 |||||||| || | ||||| | || || ||| || || |||||||| ||| | || Sbjct 59806 ATGCAGATTTTCGTGAAAACATTGACTGGAAAACACATCACCCTCGAAGTCGAACCAACA 59865 Query 24764 GACACAGTAGAAACGGTAAAACAAAAGATCCAAGACAAAGAAGGAATACCTCCCGATCAA 24823 || | ||| || ||| |||||||||||||| || ||||| || || |||||| Sbjct 59866 GATCGTATTGAAGACGTGAAAGCCAAGATCCAAGACAAGGAGGGAATTCCACCAGATCAA 59925 Query 24824 CAACGTTTGATCTTTGCCGGGAAACAGCTGGAGGATTCGAGAAACATGTC-AGATTATAA 24882 |||||| | || || ||||| || || || || || || | ||| || ||| || Sbjct 59926 CAACGTCTTATTTTCGCCGGAAAGCAACTCGAAGA-CGGAAACACACTTCAAGACTACTC 59984 Query 24883 CATTCAAAAGGAATCGACACTACACTTGGT 24912 |||||||||||| || || || ||||| || Sbjct 59985 CATTCAAAAGGATTCAACCCTCCACTTAGT 60014 >seq959 Length=42966 Score = 93.3 bits (102), Expect = 4e-14 Identities = 108/146 (74%), Gaps = 0/146 (0%) Strand=Plus/Plus Query 24704 ATGCAGATCTTTATTAAAACGCTAACCGGTAAAACTATTACGCTCGAAGTAGAAGCCACC 24763 ||||| || || | || || || || || || || || |||||||| ||| | ||| Sbjct 25278 ATGCAAATTTTCGTCAAGACACTCACTGGAAAGCACATCACACTCGAAGTCGAACCAACC 25337 Query 24764 GACACAGTAGAAACGGTAAAACAAAAGATCCAAGACAAAGAAGGAATACCTCCCGATCAA 24823 ||| | ||| || ||| || |||||||||||||||||||| || ||||||||| Sbjct 25338 GACCGTATCGAAGATGTCAAAGCCAAAATCCAAGACAAAGAAGGAATTCCACCCGATCAA 25397 Query 24824 CAACGTTTGATCTTTGCCGGGAAACA 24849 |||||| | ||||| ||||| ||||| Sbjct 25398 CAACGTCTCATCTTCGCCGGTAAACA 25423 >seq187 Length=9559 Score = 93.3 bits (102), Expect = 4e-14 Identities = 147/208 (71%), Gaps = 6/208 (3%) Strand=Plus/Plus Query 24703 GATGCAGATCTTTATTAAAACGCTAACCGGTAAAACTATTACGCTCGAAGTAGAAGCCAC 24762 |||||| |||||| |||| || || || || || || || |||||||| ||| | || Sbjct 4210 GATGCAAATCTTTGTTAAGACCCTTACAGGCAAGCACATCACCCTCGAAGTCGAACCAAC 4269 Query 24763 CGACACAGTAGAAACGGTAAAACAAAAGATCCAAGACAAAGAAGGAATACCTCCCGATCA 24822 ||| | || || ||| |||||||||||||| ||||| || || || ||||| Sbjct 4270 AGACCGTATCGAGGACGTCAAAGCTAAGATCCAAGACAAGGAAGGTATCCCACCAGATCA 4329 Query 24823 ACAACGTTTGATCTTTGCCGGGAAACAGCTGGAGGATTCGAGAAACA---TGTCAGATTA 24879 ||||||| | ||||| ||||| || || || || || || | |||| | ||| || Sbjct 4330 ACAACGTCTTATCTTCGCCGGTAAGCAACTCGAAGA--CG-GCAACACCCTCCAAGACTA 4386 Query 24880 TAACATTCAAAAGGAATCGACACTACAC 24907 ||| |||||||| || ||||| ||| Sbjct 4387 CTCCATCCAAAAGGACTCTACACTTCAC 4414 Score = 89.7 bits (98), Expect = 5e-13 Identities = 147/210 (70%), Gaps = 2/210 (1%) Strand=Plus/Plus Query 24704 ATGCAGATCTTTATTAAAACGCTAACCGGTAAAACTATTACGCTCGAAGTAGAAGCCACC 24763 ||||| ||||| | ||||| || || || || || || || ||||| ||| | || Sbjct 4439 ATGCAAATCTTCGTCAAAACCCTCACAGGAAAGCACATCACNCTTGAAGTCGAACCAACA 4498 Query 24764 GACACAGTAGAAACGGTAAAACAAAAGATCCAAGACAAAGAAGGAATACCTCCCGATCAA 24823 ||| | || || ||| |||||||||||||| ||||| || || || |||||| Sbjct 4499 GACCGTATCGAGGACGTCAAAGCNAAGATCCAAGACAAGGAAGGTATCCCACCAGATCAA 4558 Query 24824 CAACGTTTGATCTTTGCCGGGAAACAGCTGGAGGATTCGAGAAACATGTC-AGATTATAA 24882 |||||| | ||||| ||||| || || || || || | || | || || ||| || Sbjct 4559 CAACGTCTTATCTTCGCCGGTAAGCAACTCGAAGA-TGGAAACACCCTTCAAGACTACTC 4617 Query 24883 CATTCAAAAGGAATCGACACTACACTTGGT 24912 ||| ||||| || || || || |||||||| Sbjct 4618 CATCCAAAAAGACTCAACCCTTCACTTGGT 4647 >seq716 Length=195740 Score = 89.7 bits (98), Expect = 5e-13 Identities = 160/231 (69%), Gaps = 2/231 (1%) Strand=Plus/Plus Query 24704 ATGCAGATCTTTATTAAAACGCTAACCGGTAAAACTATTACGCTCGAAGTAGAAGCCACC 24763 ||||| ||||| | ||||| || || || || ||| || || ||||| ||| | ||| Sbjct 164424 ATGCAAATCTTCGTGAAAACACTCACAGGCAAGCATATCACACTTGAAGTCGAACCAACC 164483 Query 24764 GACACAGTAGAAACGGTAAAACAAAAGATCCAAGACAAAGAAGGAATACCTCCCGATCAA 24823 ||| | ||| || || || || ||||| || |||||||| || || || ||| Sbjct 164484 GACCGTATCGAAGATGTCAAGGCCAAAATTCAAGATAAGGAAGGAATTCCACCAGACCAA 164543 Query 24824 CAACGTTTGATCTTTGCCGGGAAACAGCTGGAGGATTCGAGAAACATGTC-AGATTATAA 24882 |||||||| || || || || || || || || || | || | || || |||||| Sbjct 164544 CAACGTTTAATTTTCGCTGGAAAGCAACTCGAAGA-TGGAAACACTCTTCAAGATTACTC 164602 Query 24883 CATTCAAAAGGAATCGACACTACACTTGGTGTTGCGATTGCGCGGAGGCGG 24933 |||||||||||| || ||||| ||| | || | || | || || ||||| Sbjct 164603 CATTCAAAAGGACTCAACACTCCACCTTGTTCTTCGTCTTCGTGGTGGCGG 164653 >seq576 Length=101662 Score = 89.7 bits (98), Expect = 5e-13 Identities = 148/211 (70%), Gaps = 2/211 (1%) Strand=Plus/Minus Query 24703 GATGCAGATCTTTATTAAAACGCTAACCGGTAAAACTATTACGCTCGAAGTAGAAGCCAC 24762 |||||| ||||| | || || || || || ||| || || || ||||| ||| | || Sbjct 65854 GATGCAAATCTTCGTAAAGACTCTTACTGGAAAACACATCACACTTGAAGTCGAACCAAC 65795 Query 24763 CGACACAGTAGAAACGGTAAAACAAAAGATCCAAGACAAAGAAGGAATACCTCCCGATCA 24822 || | || || ||| |||||||||||||| || ||||| || || || || Sbjct 65794 AGATCGTATCGAGGACGTCAAAGCCAAGATCCAAGACAAGGAGGGAATTCCACCAGACCA 65735 Query 24823 ACAACGTTTGATCTTTGCCGGGAAACAGCTGGAGGATTCGAGAAACATGTCA-GATTATA 24881 ||| ||||| ||||| ||||| || || || || || || | ||| ||| || || Sbjct 65734 ACAGCGTTTAATCTTCGCCGGAAAGCAACTCGAAGA-CGGAAACACACTTCAGGACTACT 65676 Query 24882 ACATTCAAAAGGAATCGACACTACACTTGGT 24912 |||||||||||| || ||||| ||||| || Sbjct 65675 CCATTCAAAAGGACTCTACACTTCACTTAGT 65645 >seq788 Length=62525 Score = 86.0 bits (94), Expect = 6e-12 Identities = 159/231 (69%), Gaps = 2/231 (1%) Strand=Plus/Minus Query 24704 ATGCAGATCTTTATTAAAACGCTAACCGGTAAAACTATTACGCTCGAAGTAGAAGCCACC 24763 ||||| || || | || || || ||||| ||| || || |||||||| ||| | || Sbjct 15358 ATGCAAATTTTCGTCAAGACACTCACCGGCAAACACATCACACTCGAAGTCGAACCAACT 15299 Query 24764 GACACAGTAGAAACGGTAAAACAAAAGATCCAAGACAAAGAAGGAATACCTCCCGATCAA 24823 || | ||| || || |||||||||||||| ||||| || || || |||||| Sbjct 15298 GATCGTATCGAAGACGTCAAGGCCAAGATCCAAGACAAGGAAGGTATCCCACCAGATCAA 15239 Query 24824 CAACGTTTGATCTTTGCCGGGAAACAGCTGGAGGATTCGAGAAACATGTC-AGATTATAA 24882 |||||| | ||||| || || || || || || || | || | ||| || ||| ||| Sbjct 15238 CAACGTCTCATCTTCGCTGGCAAGCAACTCGAAGA-TGGAAACACACTTCAAGACTATTC 15180 Query 24883 CATTCAAAAGGAATCGACACTACACTTGGTGTTGCGATTGCGCGGAGGCGG 24933 || || ||||| || ||||| ||| | || | || | || || ||||| Sbjct 15179 AATCCAGAAGGACTCAACACTTCACCTCGTCCTCCGTCTTCGTGGTGGCGG 15129 Lambda K H 0.634 0.408 0.912 Gapped Lambda K H 0.625 0.410 0.780 Effective search space used: 449889276894400